1,721,189 research outputs found
Comparison among differential Pulse Voltammetry (DPV), and amperometric biosensor and HPLC/DAD Analysis for polyphenol determinations
No full textAMPEROMETRIC SENSOR FOR PYRUVATE WITH IMMOBILIZED PYRUVATE OXIDASE
No full textSeveral procedures for immobilization of pyruvateoxidase by chemical bonding are reported. Attachment to nylon net was unsatisfactory in terms of yield and stability. Polyazetidine, a new commercially available prepolymer and a new nylon membrane with surface carboxyl groups provided good long-term stability, up to 30 days in the case of the nylon membrane. Under optimal conditions of solution preparation (0.1 mmol 1-1 thiamine pyrophosphate, 0.5 mmol 1-1 phosphate, 2.5 mmol 1-1 calcium and 0.04 mol 1-1 Tris buffer pH 7.0), linear calibration graphs were obtained until the lowest concentration of 10-3 mmol 1-1pyruvate and amperometricsensors based on these membranes. With careful standardization, the procedures were suitable for application to blood serum
Detection of pesticide in Drinking water using real-time Biospecific Interaction Analysis (BIA)
No full textAnalytical Letters
issn 0003271
Towards Fast and Inexpensive Molecular Diagnostic: The Case of p. 53
No full textMolecular diagnostic for markers detectio
Aptamers for diagnostics, environmental and food quality applications
No full textsummary of application of aptamers based bioanalytical approache
A piezoelectric quartz crystal biosensor as a direct affinity sensor
No full textDevelopment of QCM sensing in liquid
A Piezoelectric Biosensor as a Direct Affinity Sensor
No full textIt is well - known that the resonant frequency of an oscillating piezoelectric crystal can be affected by a change in mass at the crystal surface. 1 This method can be used as sensor for protein adsorption studies and for direct immunosensing. Up to now these studies have been performed by measuring the frequency in dry state, i.e. with the "dip and dry" technique which is rather cumbersome and time consuming.
Recently we obtained some results with piezoelectric crystals used directly in liquid solutions.
We will discuss a real-time monitoring of (i) adsorption of the human immunoglobulin Ig G (h -Ig G); (ii) the affinity reaction between covalently immobilized antigen (the pesticide 2,4 -D) and specific monoclonal antibodies (Mab anti- 2,4-D) from two different clones (clone F6C 10 and clone E2G2); and, (iii) immunoreactions between immobilized antigen and antibodies performing a competitive assay.
All experiments show how the reaction under study is linked to a mass increase which can be monitored continuously in real time. Direct antigen - antibody interaction can, thus, be studied without any kind of label.
A microprocessor controlled piezoelectric detector as sensor was employed to monitor in real time protein adsorption and immunoreactions using piezoelectric quartz crystals (AT-cut) with basic resonant frequency of 10 MHz. The adsorbed protein was an immunoglobulin (h -Ig G); in the immunosensing a covalent immobilized molecule (the
pesticide 2,4 -D) formed the receptor for the immobilized ligand sample (Mab anti- 2,4- D) in a competitive assay
Enzyme immunoassay with amperometric flow-injection analysis using horseradish peroxidase as a label. Application to the determination of polychlorinated biphenyls
No full text- …
