32 research outputs found
Role of KLF4 in regulation of myocardin induced SMC differentiation in human smooth muscle stem progenitor cells (hSMSPC)
No full textThe differentiation of stem cells into multiple lineages has been explored in vascular regenerative medicine. However, in the case of smooth muscle cells (SMC), issues exist concerning inefficient rates of differentiation. In stem cells, multiple repressors potentially downregulate myocardin, the potent SRF coactivator induced SMC transcription including Krüppel like zinc finger transcription factor-4 (KLF4). This thesis aimed to explore the role of KLF4 in the regulation of myocardin gene expression in human smooth muscle stem/progenitor cells (hSMSPC), a novel circulating stem cell identified in our laboratory which expresses low levels of myocardin and higher levels of KLF4. hSMSPC cells cultured in SmGM2 1% FBS with TGF-β1 (5 ng/ml “differentiation media”) show limited SMC cell differentiation potential. Furthermore, myocardin transduced hSMSPC cells cultured in differentiation media induced myofilamentous SMC like cells with expression of SM markers. Five potential KLF4 binding sites were identified in silico within 3.9Kb upstream of the translational start site of the human myocardin promoter. Chromatin immunoprecipitation assays verified that endogenous KLF4 binds the human myocardin promoter at -3702bp with Respect to the translation start site (-1). Transduction of lentiviral vectors encoding either myocardin cDNA (LV_myocardin) or KLF4 targeting shRNA (LV_shKLF4 B) induced human myocardin promoter activity in hSMSPCs. Silencing of KLF4 expression in differentiation media induced smooth muscle like morphology by day 5 in culture and increased overtime with expression of SMC markers in hSMSPCs. Implantation of silastic tubes into the rat peritoneal cavity induces formation of a tissue capsule structure which may be used as vascular grafts. Rat SMSPCs integrate into, strengthen and enhance the SMC component of such tubular capsules. These data demonstrate that KLF4 directly represses myocardin gene expression in hSMSPCs, which when differentiated, provide a potential source of SMCs in the development of autologous vascular grafts in regenerative medicine
A bibliometric analysis of the Journal of Agrometeorology (JAM) from 2008 to 2022
No full textA quantitative analysis of scientific articles published in the Journal of Agrometeorology (JAM) between 2008 and 2022 was conducted using a variety of scientometric indicators. Various metrics were utilized to examine aspects including yearly research output, highly referenced sources, author rankings, contributions and profiles, cooperation trends, highly contributing nations, most cited papers, commonly searched keywords and worldwide collaboration mapping. This study employs biblioshiny for analysis and only looks at data that is available in Scopus database. With an h-index (17), a g-index (21) and 3238 total citations across the study period, the journal demonstrated considerable influence. With the greatest number of research publications (n=46) and the greatest number of citations (236), Pandey V stands out among other authors. In terms of the number of papers and citations, India emerged as the leading nation, with the Punjab Agricultural University in the lead with 744 publications. Four clusters were found by co-citation network analysis, with Allen RG being the most quoted author among them. The study also highlighted the fact that Indian authors worked together the most. This analysis is important for assessing the influence of the JAM and offers insightful information about noteworthy research trends and developments in the scientific community
Mechanism of Oxidation of (p-Substituted Phenylthio)acetic Acids with N-Bromophthalimide
No full textThe kinetics of oxidation of (phenylthio)acetic acid (PTAA) by N-Bromophthalimide (NBP) in acetonitrile-water solvent mixture at 298 K in the presence of perchloric acid has been followed potentiometrically. The reaction is first-order each in NBP and PTAA and inverse fractional-order in H+. Also, it has been found that the reaction rate is not affected by changes in ionic strength of the reaction medium or by the addition of chemicals such as phthalimide, acrylonitrile and potassium bromide. However, an increase in the water content of the solvent mixture causes an increase in the rate of reaction. These observations have been well analyzed in favour of a SN2-type mechanism, involving NBP itself as the reactive species. Effect of substituents on the reaction rate has been analysed by employing various (p-sustituted phenylthio)acetic acids. The electron-releasing substituent in the phenyl ring of PTAA accelerates the reaction rate while the electron-withdrawing substituent retards the rate. The excellently linear Hammett plot yields a large negative ρ value, supporting the involvement a bromosulphonium ion intermediate in the rate-determining step
Experimental investigation on the suitability of ozonated electrolyte in travelling-wire electrochemical machining
No full textk-Zumkeller Graphs through Splitting of Graphs
No full textLet G = (V,E) be a simple graph with vertex set V and edges set E. A 1−1 function f : V → N is said to induce a k-Zumkeller graph G if the induced edge function f ∗ : E → N defined by f ∗(xy) = f(x)f(y) satisfies the following conditions:
f ∗(xy) is a Zumkeller number for every xy ∈ E.
The total distinct Zumkeller numbers on the edges of G is k.
In this article, we compute k-Zumkeller graphs through the graph splitting operation on path, cycle and star graphs
A review on adaptive water management for climate-resilient rice: Mitigating greenhouse gas (GHG) emissions
No full textRice production is essential for global food security and socio-economic development, as it is a staple food for many people. However, low water-use efficiency/water productivity is noticed due to the high water input in the traditional transplanted rice ecosystem with stagnant water. On the other hand, climate change affects the hydrological cycle through precipitation, causing increasing water demand and major threats to the sustainability of rice cultivation and food security for the growing population. A significant need is to find out the balance between water conservation practices and their influence on greenhouse (GHG) emissions, mainly methane. This review gives insight into a comprehensive analysis of sustainable rice production systems that improve water productivity while reducing GHG emissions, a crucial gap in existing research. To overcome this, we evaluate key strategies like aerobic rice, alternate wetting and drying (AWD), direct-seeded rice (DSR), drip-irrigated rice, a system of rice intensification (SRI) and Internet of Things (IoT) based smart irrigation, highlighting the potential water use efficiency and reducing carbon footprints. Notably, we spotlight low methane-emitting rice cultivars and drought resistance right cultivars as promising low-emission rice cultivation solutions. Additionally, this article underscores the adoption of simulation models on water productivity and seasonal GHG emissions in rice. This review provides valuable insight for policymakers and researchers to optimize rice production under changing climatic conditions. This review underscores the need for effective water management practices to enhance food security while reducing environmental impacts
