3,864 research outputs found
A continuous spectrophotometric enzyme-coupled assay for deoxynucleoside triphosphate triphosphohydrolases
No full textWe describe a continuous, spectrophotometric, enzyme-coupled assay useful to monitor reactions catalyzed by nucleoside triphosphohydrolases. In particular, using Escherichia coli deoxynucleoside triphosphohydrolase (Dgt), which hydrolyzes dGTP to deoxyguanosine and tripolyphosphate (PPPi) as the enzyme to be tested, we devised a procedure relying on purine nucleoside phosphorylase (PNPase) and xanthine oxidase (XOD) as the auxiliary enzymes. The deoxyguanosine released by Dgt can indeed be conveniently subjected to phosphorolysis by PNPase, yielding deoxyribose-1-phosphate and guanine, which in turn can be oxidized to 8-oxoguanine by XOD. By this means, it was possible to continuously detect Dgt activity at 297 nm, at which wavelength the difference between the molar extinction coefficients of 8-oxoguanine (8000 M-1 cm-1) and guanine (1090 M-1 cm-1) is maximal. The initial velocities of Dgt-catalyzed reactions were then determined in parallel with the enzyme-coupled assay and with a discontinuous high-performance liquid chromatography (HPLC) method able to selectively detect deoxyguanosine. Under appropriate conditions of excess auxiliary enzymes, the activities determined with our continuous enzyme-coupled assay were quantitatively comparable to those observed with the HPLC method. Moreover, the enzyme-coupled assay proved to be more sensitive than the chromatographic procedure, permitting reliable detection of Dgt activity at low dGTP substrate concentrations
Improved insulin sensitivity associated with reduced mitochondrial complex IV assembly and activity
No full textMice lacking Surf1, a complex IV assembly protein, have 50-70% reduction in cytochrome c oxidase activity in all tissues yet a paradoxical increase in lifespan. Here we report that Surf1-/- mice have lower body (15%) and fat (20%) mass, in association with reduced lipid storage, smaller adipocytes, and elevated indicators of fatty acid oxidation in white adipose tissue (WAT) compared with control mice. The respiratory quotient in the Surf1-/- mice was significantly lower than in the control animals (0.83- 0.93 vs. 0.90-0.98), consistent with enhanced fat utilization in Surf1-/- mice. Elevated fat utilization was associated with increased insulin sensitivity measured as insulinstimulated glucose uptake, as well as an increase in insulin receptor levels (2-fold) and glucose transporter type 4 (GLUT4; 1.3-fold) levels in WAT in the Surf1-/- mice. The expression of peroxisome proliferator- activated receptor β-coactivator 1 (PGC-1α) mRNA and protein was up-regulated by 2.5- and 1.9- fold, respectively, in WAT from Surf1-/- mice, and the expression of PGC-1α target genes and markers of mitochondrial biogenesis was elevated. Together, these findings point to a novel and unexpected link between reduced mitochondrial complex IV activity, enhanced insulin sensitivity, and increased mitochondrial biogenesis that may contribute to the increased longevity in the Surf1-/- mice.-Deepa, S. S., Pulliam, D., Hill, S., Shi, Y., Walsh, M. E., Salmon, A., Sloane, L., Zhang, N., Zeviani, M., Viscomi, C., Musi, N., Van Remmen, H. Improved insulin sensitivity associated with reduced mitochondrial complex IV assembly and activity. FASEB J. 27, 1371-1380 (2013). www.fasebj.org
Data for: Ionic Additive in an Ionogel for a Large Area Long Lived High Contrast Electrochromic Device
No full textThe raw data for all the graphical figures (Figures1-4 and Figure S1) is provided
Review of Indian Allacta Saussure & Zehntner, 1895 (Blattodea: Ectobiidae Pseudophyllodromiinae), with description of three new species
No full textSenraj, M., Packiam, S. Maria, Prabakaran, S., Lucanas, Cristian C., Jaiswal, Deepa (2021): Review of Indian Allacta Saussure & Zehntner, 1895 (Blattodea: Ectobiidae Pseudophyllodromiinae), with description of three new species. Zootaxa 4920 (2): 254-266, DOI: https://doi.org/10.11646/zootaxa.4920.2.
FIGURE 1 in Parapsilorhynchus alluriensis, a new species of cyprinid fish (Teleostei: Cyprinidae) from the Eastern Ghats, of India
No full textFIGURE 1. Distribution of the species of Parapsilorhynchus in Peninsular IndiaPublished as part of Jadhav, S. S., Karuthapandi, M., Chandra, Kailash, Jaiswal, Deepa, Dinesh, K. P. & Narahari, A., 2020, Parapsilorhynchus alluriensis, a new species of cyprinid fish (Teleostei: Cyprinidae) from the Eastern Ghats, of India, pp. 563-574 in Zootaxa 4751 (3) on page 567, DOI: 10.11646/zootaxa.4751.3.8, http://zenodo.org/record/371479
Supplemental Material, sj-docx-1-tpx-10.1177_01926233211046869 - Regulatory Perspectives on Juvenile Animal Toxicologic Pathology
No full textSupplemental Material, sj-docx-1-tpx-10.1177_01926233211046869 for Regulatory Perspectives on Juvenile Animal Toxicologic Pathology by Deepa B. Rao, Alan M. Hoberman, Paul C. Brown, Aurore Varela and Brad Bolon in Toxicologic Pathology</p
Table_S1 – Supplemental material for Nonsurgical radiologic intervention for management of abdominal wall endometriosis: A systematic review and meta-analysis
No full textSupplemental material, Table_S1 for Nonsurgical radiologic intervention for management of abdominal wall endometriosis: A systematic review and meta-analysis by Adela G Cope, Deepa M Narasimhulu, Zaraq Khan, Wendaline M VanBuren, Brian T Welch and Tatnai L Burnett in Journal of Endometriosis and Pelvic Pain Disorders</p
JEPPD_AWE_PRISMA_2009_checklist – Supplemental material for Nonsurgical radiologic intervention for management of abdominal wall endometriosis: A systematic review and meta-analysis
No full textSupplemental material, JEPPD_AWE_PRISMA_2009_checklist for Nonsurgical radiologic intervention for management of abdominal wall endometriosis: A systematic review and meta-analysis by Adela G Cope, Deepa M Narasimhulu, Zaraq Khan, Wendaline M VanBuren, Brian T Welch and Tatnai L Burnett in Journal of Endometriosis and Pelvic Pain Disorders</p
Photo-polymerized films of Lithium Ion Conducting Solid Polymer Electrolyte for Electrochromic Windows (ECWs)
No full text- …
