184 research outputs found
A Great Conversation With Kathleen Digre
No full textMeagan Seay (MS): Today, we are here with Dr. Kathleen Digre. Kathleen is Professor of Ophthalmology and Neurology at the University of Utah Moran Eye Center, where she has worked her entire career
SEIPIN Isoforms Interact with the Membrane-Tethering Protein VAP27-1 for Lipid Droplet Formation
No full textArticle demonstrating that part of the function of two SEIPIN isoforms in Arabidopsis (Arabidopsis thaliana), AtSEIPIN2 and AtSEIPIN3, may depend on their interaction with the vesicle-associated membrane protein (VAMP)–associated protein (VAP) family member AtVAP27-1. The authors used a combination of biochemical, cell biology, and genetics approaches to show that AtVAP27-1 interacts with the N termini of AtSEIPIN2 and AtSEIPIN3 and likely supports the normal formation of LDs. This insight indicates that the ER membrane tethering machinery in plant cells could play a role with select SEIPIN isoforms in LD biogenesis at the ER, and additional experimental evidence in Saccharomyces cerevisiae supports the possibility that this interaction may be important in other eukaryotic systems
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Arabidopsis thaliana EARLY RESPONSIVE TO DEHYDRATION 7 Localizes to Lipid Droplets via Its Senescence Domain
No full textThis article includes a proteomics analysis of Lipid Droplets (LDs) isolated from drought-stressed Arabidopsis leaves and identifying EARLY RESPONSIVE TO DEHYDRATION 7 (ERD7) as a putative LD protein. Observations provide new insight to ERD7 and the SD-containing family of proteins in plants and suggest that ERD7 may be involved in functional aspects of plant stress response that also include localization to the LD surface
[Photograph 2012.201.OVZ001.10958]
No full textPhotograph used for a story in the Daily Oklahoman newspaper. Caption: "Here are the members of the first Oklahoma territorial legislature. Top row: W. A. McCartney, L. G. Pittman, J. L. Brown, J. Smeltzer, G. W. Gardenshire, Pres J. Foster, D. Harrady, C. Brown, R. J. Nesbitt. Second row: J. F. Linn, M. L. Bixler, secretary Martin, Governor Steele, August Speed, J. W. Howard, C. F. Howard, C. F. Grimmer. Third row: D. W. Peery, E. C. Tritt, Judge Clark, Judge Green, Judge Seay, S. Lewis, W. H. Merten. Fourth row: W. C. Adair, D. C. Farnsworth, E. H. Long, J. A. Wimberly, J. C. Post, A. N. Daniels, C. G. jones, D. W. Talbot, M. Neal, J. L. Smith, J. L. Mathews. Bottom row: W. S. Roberts, W. H. Campbell, T. R. Waggoner, I. N. Terrill, R. J. Barker, A. M. Colson, S. D. Pack, S. W. Clark, J. M. Stovall, H. G. Trosper, G. I. Currin.
J Child Sex Abus
Get PDFResearch on child sexual abuse has focused on adult revictimization and outcomes. This article examines the rate of child maltreatment revictimization among male and female children reported to child protective services for child sexual abuse and whether revictimization impacts outcomes. Using longitudinal administrative data, Cox regressions were used to examine relationships between initial report of child sexual abuse, maltreatment revictimization, and adolescent outcomes among children from poor and nonpoor families. Despite no significant differences in child sexual abuse rates between poor and nonpoor families, poor child sexual abuse victims were significantly more likely to have re-reports for maltreatment. Children with multiple reports were more likely to have negative outcomes. Interventions for child sexual abuse survivors should focus on preventing maltreatment recurrence generally and not ignore needs of male victims.5T32DA015035/DA/NIDA NIH HHS/United StatesCE001190/CE/NCIPC CDC HHS/United StatesF31 DA034442/DA/NIDA NIH HHS/United StatesR01 MH061733/MH/NIMH NIH HHS/United StatesR01MH061733/MH/NIMH NIH HHS/United StatesT32 DA015035/DA/NIDA NIH HHS/United State
J Child Poverty
Get PDFThis qualitative study examines low-income African American fathers' perceptions of their parenting role and the strategies they employ to bring up children in poor urban neighborhoods. Focus groups and individual interviews were conducted with 36 fathers who had contact with their children at least twice a month. Men in the study expressed conventional views of their fathering roles as provider, nurturer, and teacher, but placed the most emphasis on "being there" for their children, as their financial circumstances limited other forms of involvement. Many fathers felt their circumstances to be exacerbated by a hostile child-support system. They desired to teach their children alternatives to the negative practices and values they saw in their urban neighborhoods and to have the skills to prosper in mainstream society. Overall, the findings suggest that many low-income urban fathers already desire to be responsible fathers but see themselves as limited by material and structural challenges. Services and policies that promote the economic stability of low-income fathers are recommended.T32 DA015035/DA/NIDA NIH HHS/United StatesU01 CE001627/CE/NCIPC CDC HHS/United States2014-01-01T00:00:00
Exploring personality-targeted UI design in online social participation systems
Get PDFWe present a theoretical foundation and empirical findings demonstrating the effectiveness of personality-targeted design. Much like a medical treatment applied to a person based on his specific genetic profile, we argue that theory-driven, personality-targeted UI design can be more effective than design applied to the entire population. The empirical exploration focused on two settings, two populations and two personality traits: Study 1 shows that users' extroversion level moderates the relationship between the UI cue of audience size and users' contribution. Study 2 demonstrates that the effectiveness of social anchors in encouraging online contributions depends on users' level of emotional stability. Taken together, the findings demonstrate the potential and robustness of the interactionist approach to UI design. The findings contribute to the HCI community, and in particular to designers of social systems, by providing guidelines to targeted design that can increase online participation. Copyright © 2013 ACM
Child Youth Serv Rev
Get PDFThe benefits of high-quality father-child relationships for fathers and children alike are well documented. While evidence suggests parenting programs can improve the quality of father-child relationships, few fathers participate in such programs. This qualitative study aims to fill the gap in knowledge on best practices for recruiting urban African American fathers, a group of fathers with unique parenting challenges, to parenting programs. Focus groups were conducted with 29 fathers to gain their perspectives on recruitment strategies. Semi-structured interviews were also conducted with a nationwide sample of 19 fatherhood program providers to learn about their most successful recruitment strategies. Recruitment strategies based on emergent themes from the focus groups and interviews are presented here. Themes included using word-of-mouth recruitment, increasing advertising, targeting advertising specifically to urban African American fathers, providing transportation and incentives, recruiting through the courts, collaborating with other community agencies, and offering parenting programming along with other programming valued by fathers such as employment assistance. Implications for developing strategies for recruiting urban African American fathers to parenting programs are discussed.T32 DA015035/DA/NIDA NIH HHS/United StatesT32 MH019960/MH/NIMH NIH HHS/United StatesU01 CE001627/CE/NCIPC CDC HHS/United State
Generation, Isolation and Assay Methods for Human Lymphocyte Mitogenic Factor
Get PDFActivated lymphocytes secrete many products including the lymphokine human lymphocyte mitogenic factor (HLMF). In preliminary experiments lymphocytes from peripheral blood and palatine tonsils were evaluated as possible sources of HLMF by evaluating their level of activation through screening their spontaneous and concanavalin A (con A)-induced blastogenic responses. Tonsil lymphocytes (TL) were found to have high spontaneous proliferation as compared to peripheral blood lymphocytes (PBL). Cells from both sources responded to con A by undergoing a typical blastogenic response. Because TL must be obtained septically, they are frequently cultured in the presence of the antimycotic agent, Amphotericin B (Am B). Since the primary and induced blastogenesis of TL were greatly inhibited by even low concentrations of Am B, those lymphocytes were considered unacceptable sources of HLMF. In contrast to TL the induced blastogenic responses of PBL were found to be augmented by concentrations of Am B less than 5 (mu)g/ml, but the drug appeared to provide no beneficial effect on the quantity of HLMF produced by the cells. HLMF appeared to be produced optimally in the first 48 hr of culture by 10(\u277) PBL/ml, cultured in Neuman-Tytell serumless medium which had been adjusted to 5 x 10(\u27-5) M 2-mercaptoethanol, and 5-35 (mu)g con A/ml. Stability of the HLMF activity could best be maintained by immediate dialysis against 0.05 M NH(,4)HCO(,3) solution, followed by lyophilization and storage of the dried material at -80(DEGREES)C until use. Activity was retained at -80(DEGREES)C for greater than 3 months. The activity was diminished after exposure to 56(DEGREES)C for 30 min, and completely lost after treatment at 80(DEGREES)C for 10 min or 100(DEGREES)C for 5 min. HLMF was insensitive to trypsin and exposure to pH ranges 2-7. Separation of HLMF and con A blastogenic activities was accomplished by addition of ovalbumin followed by Bio-Gel P-100 column Chromatography. HLMF activity eluted in the 12,000-20,000 d and 30,000-50,000 d ranges. The lower molecular weight material was active in the pH range 3.4-4.6 as demonstrated by isoelectric focusing. The larger molecular weight fractions had a pI of 4.14 (+OR-) 0.97. HLMF activated T cells, B cells and unfractionated PBL in assay, with the T cell response being generally, but not always greater. The factor behaved in a dose dependent fashion when assayed against unfractionated PBL
Characterization and implications of host‐cell protein aggregates in biopharmaceutical processing
No full textThis is the peer reviewed version of the following article: Oh, Y. H., Becker, M. L., Mendola, K. M., Choe, L. H., Min, L., Lee, K. H., Yigzaw, Y., Seay, A., Bill, J., Li, X., Roush, D. J., Cramer, S. M., Menegatti, S., & Lenhoff, A. M. (2023). Characterization and implications of host-cell protein aggregates in biopharmaceutical processing. Biotechnology and Bioengineering, 120, 1068– 1080. https://doi.org/10.1002/bit.28325, which has been published in final form at https://doi.org/10.1002/bit.28325. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. This article will be embargoed until 3/14/2024.In the production of biopharmaceuticals such as monoclonal antibodies (mAbs) and vaccines, the residual amounts of host-cell proteins (HCPs) are among the critical quality attributes. In addition to overall HCP levels, individual HCPs may elude purification, potentially causing issues in product stability or patient safety. Such HCP persistence has been attributed mainly to biophysical interactions between individual HCPs and the product, resin media, or residual chromatin particles. Based on measurements on process streams from seven mAb processes, we have found that HCPs in aggregates, not necessarily chromatin-derived, may play a significant role in the persistence of many HCPs. Such aggregates may also hinder accurate detection of HCPs using existing proteomics methods. The findings also highlight that certain HCPs may be difficult to remove because of their functional complementarity to the product; specifically, chaperones and other proteins involved in the unfolded protein response (UPR) are disproportionately present in the aggregates. The methods and findings described here expand our understanding of the origins and potential behavior of HCPs in cell-based biopharmaceutical processes and may be instrumental in improving existing techniques for HCP detection and clearance.This work was performed under a Project Award Agreement from the National Institute for Innovation in Manufacturing Biopharmaceuticals (NIIMBL) and financial assistance award 70NANB21H002 from the US Department of Commerce, National Institute of Standards and Technology
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