4,821 research outputs found
Letter from Alfredo Casella to Michel-Dmitri Calvocoressi, undated
No full textAn undated letter from Italian composer, pianist and conductor Alfredo Casella to French musicologist and critic Michel-Dmitri Calvocoressi
A possibile role of nirK in Rhizobium sullae HCNT1.
No full textIn response to a number of environmental stresses many bacterial species, including Vibrio
vulnificus, Sinorhizobium meliloti, Micrococcus luteus, Escherichia coli and Helicobacter pylori,
enter the viable-but-not-culturable (VBNC) status (McDougald et al., 1998). In this metabolic state
they lose their ability to grow on media that usually sustain them and undergo such physiological
and morphological changes as increased resistance to several physic and chemical factors, and
changes in protein and lipid content. The recent increasing use of specific fluorescent dyes such as
Syto 9, CTC (5-cyano-2,3-di-4-tolyl-tetrazolium chloride), AO (Acridine Orange), propidium
iodide, made possible a proper identification of viability and the metabolic state of microbes
(Basaglia et al. 1997).
Sinorhizobium meliloti 41, a rhizobium nodulating Medicago sativa, enters VBNC status in
liquid microcosms when O2 is depleted from the atmosphere of the incubation mixture (Toffanin et
al., 2000; Casella et al. 2001). Plasmid-borne, firefly-derived, luciferase gene (luc) was inserted and
stably inherited in Sinorhizobium meliloti 41 (pRP4-luc) as a reporter gene. The strain obtained, S.
meliloti 41 pRP4-luc and its parental strain, served as a model system for VBNC experiments both
in vitro and in soil samples.
Rhizobium sullae, formerly Rhizobium ’hedysari’, is a nitrogen fixing bacterium that induces
symbiotic nodule formation on the legume Hedysarum coronarium (Squartini et al., 2002). Strain
HCNT1, expressing a copper-containing nitrite reductase encoded by nirK, which is closely related
to nitrite reductases in true denitrifiers, enters the same VBNC status when oxygen is limiting, but
only when nitrite is present and converted to NO. Since HCNT1 cannot grow as a denitrifier and
inactivation of nirK only resulted in the loss of NO production (Toffanin et al, 1996), the hypothesis
that expression of nirK may induce the VBNC status has been investigated.
Therefore, a comparison between the two systems, S. meliloti and R. sullae, is presented in order
to verify the possible connection of nirK with the VBNC status.
McDougald D. et al. 1998. FEMS Microbiology Ecology 25: 1-9
Basaglia M. et al. 1997. In: The biotechnology and ecological interactions of microbial inoculants".
Granada, Spain. pp. 19-20
Toffanin A. et al. 2000. Biol. Fertil. Soils. 31 (6): 484-488
Casella S. et al. 2001. ISME-9, Amsterdam (The Netherlands), p. 191
Squartini A. et al. 2002. Int. J. Syst. Evol. Microbiol. 52: 1267-1276
Toffanin A. et al. 1996. Appl. Environ. Microbiol. 62 (11): 4019-402
Letter from Alfredo Casella to Michel-Dmitri Calvocoressi, September 19, 1903
No full textA letter, dated September 19, 1903, from Italian composer, pianist, and conductor Alfredo Casella to French musicologist and critic Michel-Dmitri Calvocoressi
Testimonianze epigrafiche per una sintesi sulla proprietá imperiale nel territorio riminese
No full textNovel biotechnological approaches for utilizing carbon containing wastes to make high value products: the "ANIMPOL" Project
No full textA sustainable and value-added conversion of waste from slaughterhouses, rendering industry, and waste fractions of the biodiesel production is the aim of the project “Biotechnological conversion of carbon containing wastes for eco-efficient production of high added value products-ANIMPOL”.
ANIMPOL was founded by the Seventh Framework Programme (FP7) of the European Commission and the project has started on the 1st January 2010. The main objective is the development of an industrial process, that brings together the solution of industrial waste problems with essential alternative strategies for polymer industry. Waste streams from slaughterhouses are converted towards fatty acid esters (FAMEs, biodiesel). Subsequently the FAME fractions, that negatively influence the biodiesel properties as a fuel, are biotechnologically converted towards high-value polyhydroxyalkanoate (PHA) biopolymers. This brings together representatives of the waste producers from animal processing industry and bio-fuel industry with the polymer industry looking for alternatives technologies. Research will be done in close cooperation between academic and industrial partners and will last for 36 months. In addition to the meat processing plant Reistenhofer GesmbH and the Scottish biodiesel producer Argent Energy (UK) Limited, other industrial partners are the italian packaging manufacturer Termoplast and the german company Argus Environmental Biotechnology GmbH (responsible for the downstream processing). From the academic side TU Graz acts as project coordinator and expert on biotechnology (Prof. Braunegg). Prof. Narodoslawsky’s group from TU Graz is responsable for Life Cycle Assessment and Prof. Schnitzer’s group from the same university for the development of cleaner production processes. The group of Prof. Casella (University of Padova, Italy) will be on charge of the microbiology and genetics part and Prof. Horvat (University of Zagreb, Croazia) for the mathematical modeling of bioprocesses. The Karl-Franzens University Graz (Prof. Mittelbach) takes over the task of the optimized production of biodiesel from animal fats, while the University of Pisa (Prof. Chiellini), National the Institute of Chemistry (Ljubljana, Dr. Krzan) and the Polish Academy of Sciences (Prof. Kowalczuk) have specific tasks in the field of polymer characterization. Providing long-term strategies for long-term problems, the project will result in value creation for all players. The development of this integrated process will give results in microbiology, genetics, biotechnology, chemical engineering, polymer chemistry- and processing and life cycle analysis, combined with feasibility studies for marketing of the final products. The project activities aim at solving local waste problems affecting the entire EU; the solutions will be developed on local scales, but are meant to be applied to the entire EU and will provide cost-efficient and alternatives for polymer industry.
Information:
Prof. Sergio Casella, University of Padua, [email protected]
www.animpol.tugraz.a
La svolta galeriana prima e dopo l’editto di Serdica
No full textThe crisis of the third century helped to create conditions for a change in terms of relations between the Roman Empire and Christianity, whose spread resulted, in the upper echelons of the State, in an awareness of the extent of the phenomenon. The measures that Roman State authorities took against Christianity were a reaction to this dangerous threat; among them, in the specific case of this paper, we focus on the persecution triggered – according to Lactantius – by the Caesar Galerius under the senior Augustus Diocletian. The same Galerius as Augustus was responsible for the formal act that put an end to persecutions: the edict of Serdica (or of Nicomedia), issued on 30 April of year 311. Apart from hypothetical personal reasons, the turning point is to be read in the context of traditional imperial clementia. As a result, Galerius emerges as an autonomous and leading figure within tetrarchical legislative policy, in the light of objective circumstances that led him to adapt Diocletian’s project to the needs of the times
Continuità in (una) crisi? I motivi di una giornata di studio
No full textDettagliato e aggiornato status quaestionis sulla crisi del III° secolo con particolare attenzione al contesto balcanico-danubiano
Correction: The reaction of acetonitrile with hydrogen peroxide in alkaline medium: a DFT mechanistic study of green production of amides
No full textCorrection for 'The reaction of acetonitrile with hydrogen peroxide in alkaline medium: a DFT mechanistic study of green production of amides' by Girolamo Casella et al., Phys. Chem. Chem. Phys., 2023, https://doi.org/10.1039/d3cp02024j
Sviluppo di un metodo per il dosaggio plasmatico del busulfan mediante HPLC-ESI-MS/MS in studi di farmacocinetica su pazienti talassemici
No full textL'ottimizzazione del dosaggio busulfan in pazienti sottoposti a trapianto di midollo osseo si raccomanda al fine di ridurre gli effetti tossici associati all’elevata esposizione al farmaco. Una variabilità dell'area sotto la curva di concentrazione / tempo (AUC) comporta il rischio di sovra o sotto dosaggio con il conseguente aumento del rischio di tossicità o di recidiva.
E’ stato sviluppato un metodo di analisi rapido, sensibile e specifico per il rilevamento del busulfan nel plasma umano. Il test si basa su una estrazione liquido-liquido con acetato di etile e di rilevamento mediante cromatografia liquida, con ionizzazione electrospray e spettrometria tandem massa (HPLC-ESI-MS/MS). Il busulfan deuterato è stato utilizzato come standard interno.
Il metodo è lineare nel range 39-2500 ng / mL, con r2> 0,99 e una durata della corsa cromatografica di soli 5 minuti (con un tempo di ritenzione del busulfan di 1,62 minuti). La precisione inter-day and intra-day è nel range di 1,01 - 3,72% e di 0,35-4,32%, rispettivamente. Il recupero è > 70%. Il limite di rivelabilità e di quantificazione sono di 6 e 10 ng/mL, rispettivamente. Il metodo di analisi riportato, richiede soltanto 200 μL di plasma per l'analisi.
Il metodo convalidato è stato applicato con successo per l’analisi di campioni di plasma ottenuti da bambini talassemici sottoposti a un regime di condizionamento con busulfan e sottoposti a trapianto di midollo osseo. Questo metodo permette la correzione della dose consentendo un dosaggio adeguato al fine di ottenere la concentrazione ottimale di busulfan.
Questo metodo è attualmente utilizzato per analizzare le concentrazioni plasmatiche di busulfan dopo somministrazione per via endovenosa ed è applicato nel monitoraggio terapeutico dei farmaci.Optimisation of busulfan dosage in patients undergoing bone marrow transplantation is recommended in order to reduce toxic effects associated with high drug exposure. Variation in the area under the concentration /time curve (AUC) results in risk of over or under treatment with excess risk of toxicity or relapse.
A rapid, sensitive and specific assay for detection of busulfan in human plasma was developed. The assay is based on a liquid-liquid extraction with ethyl acetate and detection by high performance liquid chromatography with electrospray ionization and tandem mass spectrometry (HPLC-ESI-MS/MS). Deuterated busulfan was used as internal standard.
The method was linear over the range 39-2500 ng/mL, with r2 > 0.99 and a run time of only 5 minutes (busulfan retention time of 1.62 minutes). The inter-day and intra-day precision were in the range 1,01- 3,72 % and 0,35-4,32 %, respectively. The recovery was >70%. The limits of detection and quantification were 6 and 10 ng/mL, respectively. The reported assay required only 200 µL of plasma for the analysis.
The validated method was successfully applied to analyze plasma samples from children with thalassemia undergoing a conditioning regimen with busulfan and submitted to bone marrow transplantation. This method permits dose correction allowing a better dosing adjustment towards the target level of busulfan.
This method is currently used to analyze the plasma concentrations of busulfan after intravenous administration and it is applied in therapeutic drug monitoring
FRA COSTANTINO E I VANDALI. Atti del Convegno Internazionale di Studi per Enzo Aiello (1957-2013), Messina, 29-30 Ottobre 2014, L. De Salvo, E. Caliri, M. Casella (a cura di), MUNERA 40, Edipuglia, Bari- S.Spirito 2016.
No full textThis book brings together the papers presented at the Conference held in Messina at the Department of Ancient and Modern Civilisations on 29 and 30 October 2014 in memory of Vincenzo Aiello. On this occasion, scholars of various nationalities and scientific backgrounds remembered the young colleague who died prematurely with contributions on topics particularly close to him.
The papers were divided into two sections: the first on the figure of Constantine, his Fortleben and the political and religious dynamics of the 4th century; the second on the impact of the presence of the Vandals in the Mediterranean basin in the 5th century and the new political geography determined by the conquest of Africa.
The collected essays offer original contributions to the archaeological, numismatic, historical-institutional, historical-religious, juridical and economic issues of the periods in question, and contribute to a richer picture in terms of ideas and results for future research in these fields
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