1,721,109 research outputs found
Paratracheal cervical mass in a dog
No full textA 14-year-old female Welsh Terrier was presented for evaluation of multiple skin masses. Two of them were diagnosed as a follicular (epidermal) cyst and a sebaceous epithelioma by cytology and histopathology. The third mass was located in the subcutis adjacent to the cervical trachea. Clinical findings, thoracic radiography, and laboratory results were otherwise unremarkable. Cytologically, the cervical mass was characterized by the presence of round to polygonal cells with distinct cell borders, mild to moderate anisocytosis, round to oval eccentric nuclei with prominent nucleoli, and a variable amount of finely granular, eosinophilic cytoplasm. Differential diagnoses included endocrine/neuroendocrine tumors such as thyroid adenocarcinoma, medullary thyroid carcinoma, carotid body chemodectoma, and parathyroid carcinoma. The mass was removed surgically and submitted for histopathologic and immunohistochemical examination. Histologically, the mass was composed of rows and nests of densely packed cells separated by thin fibrovascular stroma. Neoplastic cells had moderate to large amounts of pale eosinophilic dusty or finely vacuolated cytoplasm and round to oval nuclei with finely dispersed chromatin and 1 or 2 small nucleoli. On immunohistochemistry, neoplastic cells were positive for chromogranin A, calcitonin, and neuron-specific enolase, and were negative for cytokeratin and thyroglobulin. The final diagnosis was medullary thyroid carcinoma, an uncommon endocrine tumor of dogs that has cytologic and histopathologic features similar to other endocrine and neuroendocrine tumors. Immunohistochemistry or immunocytochemistry should be performed to differentiate medullary thyroid carcinoma from other endocrine/neuroendocrine tumors of dogs. (Bertazzolo W, Giudice C, Dell'Orco M, Caniatti M. Paratracheal cervical mass in a dog [medullary thyroid carcinoma]
Immunohistochemical detection of papillomavirus structural antigens in animal hyperplastic and neoplastic epithelial lesions
No full textOne hundred and seventy-two hyperplastic and neoplastic epithelial lesions from 8 different mammalian and 1 avian species have been tested with an immunohistochemical technique to detect papillomavirus structural antigens. Selected lesions were diagnosed histologically as papilloma, fibropapilloma, equine sarcoid, squamous cell carcinoma, basalioma, epulis, keratoacanthoma, trichoepithelioma, pilomatrixoma, epidermal inclusion cyst, and hyperkeratotic or acanthotic epidermal lesions. Positive nuclear staining was detected in 14 out of 23 papillomas, 8 out of 32 fibropapillomas and in 1 out of 3 hyperplastic epidermal lesions. Positive samples were found in 5 of 8 mammalian species. Selected samples were also examined by transmission electron microscopy. In 4 samples papillomavirus was seen. In two other samples, negative with immunoperoxidase technique, papovavirus-like particles were observed
Cytologic study of normal canine testis
No full textIt has been shown that cytology has a role in the diagnosis of canine infertility, but there are few reports dealing with normal cytologic features of the testis. This is in contrast with human medicine, in which the normal testicular cytology has been thoroughly described and assessed both by qualitative and quantitative approaches. This study was performed to provide a description of the normal cells in cytologic specimens of the testis and to establish ratios between cells that quantify testicular function. Six male dogs (Canis lupus familiaris) with no testicular pathology, aged 3 to 8 yr, were subjected to elected orchiectomy and included in this study. For each testis, six impression smears were obtained, air dried, and stained with a rapid Romanowsky-type stain. Specimens presented low blood contamination and a heterogeneous population of isolated or loosely packed cells, with high pleomorphism and anisokaryosis; naked nuclei, smeared chromatin, and multinucleation were frequent. The morphologic features of the normal testicular cells from spermatogonia to spermatozoa, including Sertoli and Leydig cells, were described. Cells were classified according to slightly modified criteria taken from human cytology. Simultaneously, 500 cells were counted per slide to determine the sperm index (SI) and Sertoli cell index (SEI), which were 26.6+/-3.8 and 4.2+/-0.8, respectively. In general, the cells encountered in the dog resemble those of men, although some particularities exist, such as the much larger size of the Sertoli cells, the highly vacuolated appearance of Leydig cells, or the lower SI and SEI in dogs. The descriptions and indexes defined here may assist in the interpretation of a testicular cytolog
SIGNIFICATO PROGNOSTICO DELLA BIOPSIA ECOGUIDATA CON AGO SOTTILE DI MILZA E FEGATO NELLA STADIAZIONE CLINICA DEL MASTOCITOMA DEL CANE
No full textCNS microgliomatosis: pathological findings in a dog
No full textAIM OF THE STUDY: To characterize a case of multifocal microgliomatosis (MCGs) in a dog. MCGs is a CNS tumor of the white matter described in humans and dogs. In the WHO classification MCGs has been included among hystiocytic disorders. However, it’s derivation from the phagocytic microglia has never been fully demonstrated. MATERIALS AND METHODS: A 13 years old, male castrated, Boxer presented with ataxia and behavioural disorders progressing into seizures. Thomograpy revealed two lesions in the left and right cerebral emyspheres. The dog was humanely killed, the brain was removed and examined grossly and microscopically. Immunohistochemistry with anti-canine CD18 and CD45RA and antibodies recognizing vimentin, cytokeratin(AE1/AE3), GFAP, S-100, NSE, CD3 and CD79a was performed. RESULTS: Gross CNS findings were unremarkable. Histology revealed a multifocal mixed proliferative disorder substituting and blending into the white matter of composed of short interlacing and arborizing bundles of atypical spindle cells admixed with gitter cells, mature lymphocytes and granulocytes. No ground substance was present. No angiocentric pattern was evidenced. Spindle cells were vimentin and CD18 positive suggesting a leukocyte origin. All other markers were negative excluding the differential diagnoses such as lymphoma, meningioma, astrocytoma and neuroendocrine tumors. CD18 positive gitter cells and neutrophils were evidenced. Lymphocytes within lesion were mostly CD3 positive mature T cells. CONCLUSION: Our findings suggest tha MCGs is an immunoproliferative disorder resembling the mixed patter of reactive hystiocytoses where the spindle cells bear a phenotype indicative of a leukocyte (non-lymphoid) origin most likely of the CNS microglia
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