560 research outputs found
Supplemental material for A 6-month open-label extension study of the safety and efficacy of subcutaneous belimumab in patients with systemic lupus erythematosus
No full textSupplemental material for A 6-month open-label extension study of the safety and efficacy of subcutaneous belimumab in patients with systemic lupus erythematosus by A Doria, D Bass, A Schwarting, A Hammer, D Gordon, M Scheinberg, NL Fox, J Groark, W Stohl, C Kleoudis and D Roth in Lupus</p
Sustainable valorisation of organic urban wastes : insights from African case studies
No full textUnderstanding the problems and potentials of the organic waste stream is perhaps the single most important step that city authorities in Africa could take in moving towards sustainable, affordable, effective and efficient waste management. This publication presents four examples of recent attempts to manage organic waste sustainably in the African context. The participants in the ‘Nairobi organic urban waste’ project have structured this case exercise in order to use the case studies as object lessons, to harvest genuine insights into the feasibility of a variety of ways to successfully and sustainably valorise urban organic waste streams. Three contemporary case examples of compost production are presented. These include composting by a community-based organisation in the Kenyan private sector and by a public-private partnership in Malawi. In all three cases, the project and case study focus is on the relations between city waste and the agricultural supply chain. A fourth case study describes the technical and economic potential to produce and use biogas from urban organic waste
A remark on the approximation theorems of Whitney and Carleman-Scheinberg
No full textsummary:We show that a -smooth mapping on an open subset of , , can be approximated in a fine topology and together with its derivatives by a restriction of a holomorphic mapping with explicitly described domain. As a corollary we obtain a generalisation of the Carleman-Scheinberg theorem on approximation by entire functions
Directed therapy for patients with myelodysplastic syndromes (MDS) by suppression of cyclin D1 with ON 01910.Na
No full textAbstract not availableMatthew J. Olnes, Aarthie Shenoy, Barbara Weinstein, Loretta Pfannes, Kelsey Loeliger, Zachary Tucker, Xin Tian, Minjung Kwak, Francois Wilhelm, Agnes S.M. Yong, Irina Maric, Manoj Maniar, Phillip Scheinberg, Jerome Groopman, Neal S. Young, Elaine M. Sloan
Are we ready to replace anti TNF with anti IL12-23 in Psoriasis and Psoriatic Arthritis
No full textFil: Pereira Almeida De Piano, Luciana. Hospital Abreu Sodré; BrasilFil: Prado Golmia, Ricardo. Hospital Abreu Sodré; BrasilFil: Scheinberg, Morton A. Hospital Abreu Sodré; Brasi
IgM monoclonal antibody JD118 recognizes an inducible antigen target for human-complement-mediated cytotoxicity against neoplastic B cells.
No full textSpecific human cellular immunity to bcr-abl oncogene-derived peptides
No full textChronic myelogenous leukemia (CML) cells are characterized by a t(9;22) translocation, which can encode one of two chimeric P210 bcr-abl fusion proteins, comprising products of either the b2a2 or the b3a2 exon junction. The junctional sequences represent potentially immunogenic tumor-specific antigens. Despite their intracellular location, the fusion proteins might be recognized immunologically by T lymphocytes if peptides, derived from these unique sequences, are capable of presentation by the major histocompatibility complex molecules. We previously found that four peptides, 9 to 11 amino acids long, spanning the b3a2 CML breakpoint bind with high or intermediate affinity to purified HLA class I molecules A3, A11, B8, or both A3 and A11. We tested the ability of these peptides to elicit specific class I restricted cytotoxic T lymphocytes (CTLs) in vitro in HLA-matched healthy donors. In addition, a longer b3a2 CML-breakpoint-derived peptide, 25 aminoacids in length (b3a2-25), was studied for its ability to induce peptide-specific, class II-mediated, T-cell proliferation. In four of four HLA-A3 donors tested, CML-A3/A11-peptide specific CTLs were induced that killed an allogeneic HLA-A3-matched peptide pulsed leukemia cell line. In two of three HLA-A3 donors, the CML-A3/A11 peptide was able to induce killing of autologous and allogeneic HLA-matched peptide-pulsed peripheral blood mononuclear cells (PBMC). CML-A3 peptide induced peptide specific CTLs in one of the four HLA A3 donors tested. No killing was observed in two HLA-B8 and two HLA-A11 donors. PBMC from seven donors were also tested for anti b3a2-25 peptide proliferation in a thymidine incorporation assay. Specific proliferation was detected in three donors, all of the HLA-DR11 haplotype. These data represent the first evidence of a cytolytic human immune response against CML bcr-abl oncogene-derived peptides and provide a rationale for developing peptide-based vaccines for this disease
PCPro, plasma lipid biomarker, in metastatic hormone-sensitive prostate cancer (mHSPC): from research to clinical implementation in the ENZAMET trial (ANZUP 1304)
No full textR. Mellor, M. Fitzpatrick, T. Scheinberg, R.M. Kim, P. Bonnitcha, D. Sullivan, H-M. Lin, I.D. Davis, A.J. Martin, P. Meikle, A.M. Joshua, M. Mcjannett, V. Subhash, S. Yip, S. North, R. McDermott, K.N. Chi, M.R. Stockler, C. Sweeney, L.G. Horvat
Specific binding of leukemia oncogene fusion protein peptides to HLA class I molecules
No full textMany human leukemias are characterized by chromosomal translocations yielding hybrid RNAs capable of encoding fusion chimeric proteins. The unique amino acid sequences found in these oncogenic fusion proteins represent true tumor-specific antigens that are potentially immunogenic. Although these leukemia-specific fusion proteins have an intracellular location, they might be recognized immunologically by T lymphocytes if peptides derived from the unique sequences are capable of presentation by the major histocompatibility complex (MHC) molecules on leukemic cells. The ability of a series of synthetic peptides corresponding to the junctional sequences of chronic myelogenous leukemia (CML)-derived bcr-abl and acute promyelocytic leukemia (APL)-derived PML-RAR alpha fusion proteins to bind to purified class I molecules was studied. A series of 152 peptides 8, 9, 10, and 11 amino acids in length, spanning the b3a2 and b2a2 breakpoints for CML and PML-RAR alpha A and B breakpoints for APL were analyzed for HLA A1, A2.1, A3.2, A11, A24, B7, B8, and B27 binding motifs. Twenty-one CML peptides and 4 APL peptides were predicted to be potential HLA class I binders. The peptides were tested for binding to appropriate purified HLA molecules in a competition radioimmunoassay. Four peptides derived from b3a2 CML breakpoint bound with high (<50 nmol/L) or intermediate (less than or equal to 500 nmol/L) affinity to HLA A3, A11, and B8. None of the CML b2a2 or PML-RAR alpha A or B junctional peptides showed affinity of this magnitude for the HLA class I molecules tested. This is the first evidence that tumor-specific breakpoint peptides can bind human MHC class I molecules and provides a rationale for developing a therapeutic vaccine strategy. (C) 1995 by The American Society of Hematology
Modulation of p53, WAF1/p21 and bcl-2 expression during Retinoic Acid-induced differentiation of NB4 promyelocitic cells
No full textHeat shock protein 70 (HSP70) protects cells from various injurious stimuli and is important in cell growth and differentiation. However, its expression in leukemia cells has not been analyzed systematically. We, therefore, investigated the expression of HSP70 in various types of leukemia cells and hematopoietic cell lines. Immunoblot analysis revealed that HSP72/73 were expressed at low levels in the acute lymphocytic leukemia cells and lymphoid cell lines examined. However, heat (43°C for 2 h) preferentially augmented HSP72/73 expression in lymphoid cells. This induction was partially blocked by the treatment with quercetin (10 μM for 24 h). Finally, heat shock following quercetin treatment synergistically induced apoptosis in a lymphoid cell line (OZ). These observations suggest the possibility of selective purging of lymphocytic leukemia cells by combination with quercetin and heat
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