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Caratterizzazione morfologica della reattività agli anticorpi monoclonali B 6.2, B 72.3 e B 1.1 nella displasia e nel cancro della mammella
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Morphologic assessment of reactivity to monoclonal antibodies generated against breast cancer cells in mammary tissues removed for clinical dysplasia or cancer
No full textMonoclonal antibodies B 6.2, B 72.3 and B 1.1, reactive against tumor-associated antigens of human breast cancer, have been tested on histologic sections of paraffin blocks from 49 cases of breast cancer and from 13 cases of mammary dysplasia. The results have shown that: (a) the three monoclonal antibodies recognize different antigenic determinants of the cancer cells; (b) monoclonal antibodies B 6.2 and B 1.1 recognize antigens which are frequently expressed in cancer cells; (c) the antigen recognized by monoclonal antibody B 72.3, at the concentrations used, is less frequently expressed in tumors, while it seems to be selective for the apical cell border and luminal secretions of apocrine metaplastic lobules or cysts occurring in mammary dysplasia; (d) intracytoplasmic expression of the antigens is observed only in cancer cells, with rare exceptions; (e) tumor cell populations are heterogeneous in respect to the expression of the antigens detected by the monoclonal antibodies tested; (f) the antigens detected are unrelated to the tumor histotype; (g) the frequency of axillary lymph nodal metastases is not influenced by the presence or intensity of antigenic expression in the primary tumors; but (h) in metastatic tumor cell populations the antigenic expression is higher than in primary tumors. This fact suggests some relationship between tumor metastatization and the expression of antigens recognized by the monoclonal antibodies teste
Application of monoclonal antibodies in the recognition of tumor-associated antigens in human breast
No full textAlta specificità di un nuovo anticorpo monoclonale MM1.80, riconosciuto da un antigene tumore associato al carcinoma mammario umano e alle relative metastasi
No full textA tumor - associated antigen recognized by a monoclonal antibody on apocrine metaplasia of human breast
No full textImmunohistochemical characterization of a novel monoclonal antibody MM1.80 recognizing a tumor associated antigen in breast carcinomas
No full textMammary cancer antigen recognized by monoclonal antibody B72.3 in apocrine metaplasia of the human breast
No full textMonoclonal antibody B72.3 recognizing a pan-associated carcinoma antigen expressed also in metastatic human breast cancer cells has been tested using the avidin-biotin peroxidase method applied to paraffin-embedded sections in 50 samples of mammary tissue showing apocrine metaplasia and in 58 cases of other mild or severe focal epithelial proliferative changes of the breast, including mostly in situ lobular or ductal carcinomas collateral to clinical cancer removed after radical mastectomy. The antigen detected by this antibody was present in the apocrine cells of 48 cases (96%). In the majority of these cases the reactivity was localized on the luminal border of the apocrine cells and in the luminal secretion. But ten cases showed positive staining also in the cell cytoplasm either focal or diffuse. The normal structures and mild focal hyperplastic changes collateral to clinical cancer were, in the majority of the cases (43 of 55), negative, and, when positive, displayed positivity only at the luminal border. By contrast, the independent foci of in situ carcinoma (17 of 31 examined), the intraduct papillomas (seven cases of 14), and the intraductal component of breast carcinoma (seven cases of 17) were positive, displaying a cytoplasmic focal or diffuse staining. In conclusion, mammary apocrine metaplasia, a metaplastic change of the normal epithelium that has been associated with increased breast cancer risk, shares antigens in common with breast cancer cells and/or with cells showing severe atypia. The possible clinical significance of the site of antigenic expression (cytoplasm or luminal border) needs further investigation
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