288 research outputs found
La parola narrativa come luogo della memoria in Erri De Luca
No full textLa parola narrativa come luogo della memoria; oralità e scrittura nei romanzi di Erri De Luc
Shakespeare e Platone: Eros e creazione poetica nei Sonetti
No full textInfluenza del Simposio di Platone sui Sonetti di Shakespeare; rapporto tra eros e creazione poetica; la poesia come "parto nell'anima
PWA of the e"+e"- #-># #PI#"+#PI#"-#PI#"+#PI#"- reaction in the #rho#'(1600) mass range
No full textPresented by Luca Stanco to Hadron 91, Maryland University, Aug. 12-16, 1991Available at INIST (FR), Document Supply Service, under shelf-number : RP 10960 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueSIGLEFRFranc
A new way to determine the neutrino mass hierarchy at reactors
No full textThe determination of the neutrino mass ordering is currently pursued by several experiments and proposals. A very challenging one is its evaluation from reactor experiments based on the tiny interference effect between the Δm231 and Δm232 oscillations. Current analyses require several years of data taking and an extreme energy resolution to achieve anyhow less than 5 σ. Referring to the JUNO experimental conditions we developed a completely new technique that would provide a robust 5 σ measurement in less than six years of running. The two orderings could be discriminated at the price of allowing for two different values of Δm231. This degeneracy on Δm231 (around 12×10−5 eV2) can however be measured at an unprecedented accuracy of much less than 1\%, i.e. 10−5 eV2, within the same analysis. Analogies with the usual χ2 analysis, where the Δm231 degeneracy is much more important, are discussed. Evaluation and inclusion of systematic errors and backgrounds have been performed, the most relevant among them being the addition of the two remote reactor plants 250 km away. Baselines of each contributing reactor core and its spatial resolution have been taken into account. Possible results after two years of running and the foreseen initially-reduced available reactor power have been studied, too. These results confirm the very positive perspectives for JUNO to determine the mass ordering in a vacuum-oscillation dominated region
Tra natura e cultura. L'improbabile formazione di Katherina in The Taming of the Shrew
No full textL'ideale di formazione nel Rinascimento: dalla base 'naturale' del carattere alla possibilità di una modellizzazione 'culturale' della personalità. L'esempio di Katherina in The Taming of the Shrew di William Shakespear
Qubit4Sync: a qubit-based synchronization system for quantum key distribution
No full textQubit4Sync is a synchronization method for quantum key distribution which uses the same qubits exchanged during the protocol; in the same spirit of classical communication protocols reling on clock recovery methods that uses the data stream itself
Tenogenic differentiation protocol in xenogenic-free media enhances tendon-related marker expression in ASCs
No full textAdipose-derived stem cells (ASCs) are multipotent and immune-privileged mesenchymal cells, making them ideal candidates for therapeutic purposes to manage tendon disorders. Providing safe and regulated cell therapy products to patients requires adherence to good manufacturing practices. To this aim we investigated the in vitro tenogenic differentiation potential of ASCs using a chemically defined serum-free medium (SF) or a xenogenic-free human pooled platelet lysate medium (hPL) suitable for cell therapy and both supplemented with CTGF, TGFβ-3, BMP-12 and ascorbic acid (AA) soluble factors. Human ASCs were isolated from 4 healthy donors and they were inducted to differentiate until 14 days in both hPL and SF tenogenic media (hPL-TENO and SF-TENO). Cell viability and immunophenotype profile were analysed to evaluate mesenchymal stem cell (MSC) characteristics in both xenogenic-free media. Moreover, the expression of stemness and tendon-related markers upon cell differentiation by RT-PCR, protein staining and cytofluorimetric analysis were also performed. Our results showed the two xenogenic-free media well support cell viability of ASCs and maintain their MSC nature as demonstrated by their typical immunophenototype profile and by the expression of NANOG, OCT4 and Ki67 genes. Moreover, both hPL-TENO and SF-TENO expressed significant high levels of the tendon-related genes SCX, COL1A1, COL3A1, COMP, MMP3 and MMP13 already at early time points in comparison to the respective controls. Significant up-regulations in scleraxis, collagen and tenomodulin proteins were also demonstrated at in both differentiated SF and hPL ASCs. In conclusion, we demonstrated firstly the feasibility of both serum and xenogenic-free media tested to culture ASCs moving forward the GMP-compliant approaches for clinical scale expansion of human MSCs needed for therapeutical application of stem cells. Moreover, a combination of CTGF, BMP-12, TGFβ3 and AA factors strongly and rapidly induce human ASCs to differentiate into tenocyte-like cells
Time-bin Quantum Key Distribution exploiting the iPOGNAC polarization moulator and Qubit4Sync temporal synchronization
No full text3D Bioprinting of Human Adipose-Derived Stem Cells and Their Tenogenic Differentiation in Clinical-Grade Medium
No full textDefining the best combination of cells and biomaterials is a key challenge for the development of tendon tissue engineering (TE) strategies. Adipose-derived stem cells (ASCs) are ideal candidates for this purpose. In addition, controlled cell-based products adherent to good manufacturing practice (GMP) are required for their clinical scale-up. With this aim, in this study, ASC 3D bioprinting and GMP-compliant tenogenic differentiation were investigated. In detail, primary human ASCs were embedded within a nanofibrillar-cellulose/alginate bioink and 3D-bioprinted into multi-layered square-grid matrices. Bioink viscoelastic properties and scaffold ultrastructural morphology were analyzed by rheology and scanning electron microscopy (SEM). The optimal cell concentration for printing among 3, 6 and 9 × 106 ASC/mL was evaluated in terms of cell viability. ASC morphology was characterized by SEM and F-actin immunostaining. Tenogenic differentiation ability was then evaluated in terms of cell viability, morphology and expression of scleraxis and collagen type III by biochemical induction using BMP-12, TGF-β3, CTGF and ascorbic acid supplementation (TENO). Pro-inflammatory cytokine release was also assessed. Bioprinted ASCs showed high viability and survival and exhibited a tenocyte-like phenotype after biochemical induction, with no inflammatory response to the bioink. In conclusion, we report a first proof of concept for the clinical scale-up of ASC 3D bioprinting for tendon TE
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