13 research outputs found
Biological activity of enantiomeric complexes [PtCl2L2](L2 is aromatic bisphosphanes and aromatic diamines)
No full textEnantiomeric complexes of formula [PtCl(2)L(2)] [L(2) is (R)-(+)-BINAP and (S)-(-)-BINAP, where BINAP is 2,2'-bis(diphenylphosphane)-1,1'-binaphthyl, and (R)-(+)-DABN and (S)-(-)-DABN, where DABN is 1,1'-binaphthyl-2,2'-diamine], were tested for their cytotoxic activity against three cancer cell lines and for their ability to bind to the human telomeric sequence folded in the G-quadruplex structure. Similar experiments were carried out on prototypal complexes cisplatin and cis-[PtCl(2)(PPh(3))(2)] for comparison. Platinum complexes containing phosphanes proved less cytotoxic to cancer cell lines and less likely to interact with the nucleobases of the G-quadruplex than those containing amines; in both cases the S-(-) isomer was more active than the R-(+) counterpart. More specifically, whereas all the platinum complexes were able to platinate the G-quadruplex structure from the human telomeric repeat, the extent and sites of platination depended on the nature of the ligands. Complexes containing (bulky) phosphanes interacted only with the adenines of the loops, whereas those containing the less sterically demanding amines interacted with adenines and some guanines of the G-quartet
Fournier's gangrene: a clinical presentation of necrotizing fasciitis after bone marrow transplantation.
No full textThree patients with ANLL developed Fournier's gangrene as an early complication after allo-BMT (two cases) and auto-BMT (one case); two patients were in first CR, the third had resistant disease. Patients developed fever, perineal pain, swelling and blistering of the genital area. Pseudomonas aeruginosa was isolated from the lesions and patients received systemic antibiotic therapy, surgical debridement and medication with potassium permanganate solution. Two patients made a complete recovery although one died of sepsis. The third had progressive involvement of the abdominal wall and later died of leukemia. Early diagnosis of this disorder and prompt initiation of appropriate therapy can prevent progression of this acute necrotizing infection
LCA and wild animals: Results from wild deer culled in a northern Italy hunting district
No full textAlthough the research of innovative and sustainable environmental alternatives to meat consumption is increasing, little attention has been given to hunting activity, which has traditionally provisioned food products from wild animals. Given this gap, the present study aims to quantify the environmental impacts of wild red deer culling (Cervus elaphus) through selective hunting in a mountainous Italian district, adopting a cradle-to-gate life cycle assessment (LCA) approach. Nine impact categories are evaluated using the International Reference Life Cycle Data System (ILCD) v1.09 impact assessment method, with climate change filling a special role. The results highlight that the long distances covered by the hunters to cull wild red deer is the hotspot of the supply chain representing almost 85% of the impact in every considered impact categories. Focusing on climate change, the outcomes show that the emissions of greenhouse gases (GHGs) per functional unit (4.85 kg CO2eq) are largely influenced by the hypothesis considering the wild red deer as an elementary flow entering the system and, thus, not including enteric methane emissions. In this case, the hunted red deer meat appears to be an environmentally sustainable alternative to conventional beef. The representativeness of the findings has to be increased both within the same species and in association with other wild ungulates (e.g., roe deer, wild boar or chamois) to better understand the potential role of traditionally hunted wild products in more sustainable diets
Scelte pianificatorie e valorizzazione del capitale naturale. Un caso di studio in Lombardia: la futura provincia di Monza e Brianza
No full textScelte pianificatorie e valorizzazione del capitale naturale. Un caso di studio in Lombardia: la futura provincia di Monza e Brianz
2024 ASCA International Conference on Applied Strength and Conditioning
No full textBLUF:
Wearing upper body blood flow restriction cuffs increases bench press barbell velocity of moderate to high loads.
INTRODUCTION: Resistance exercise (RE) is important for athletes to enhance muscular strength and power. Traditional RE necessitates loads exceeding 60% of one repetition maximum (1RM) is required for stimulating muscular hypertrophy and strength, which also correlate with improved physical performance metrics. To maximise the outcome of a RE training session, and improve longitudinal physical performance metrics, the addition of barbell velocity feedback, and training at faster barbell velocities are preferred. Consequently, athletes and strength coaches seek innovative RE methodologies to enhance barbell velocity for strength and performance improvements. One potential method involves the athlete wearing blood flow restriction (BFR) cuffs during the RE session. While research has focused primarily on low-load BFR-RE, studies indicate that moderate-to-high load BFR enhances barbell velocity in squats and bench press (BP) compared to non-BFR training. Therefore, the aim of this case-study was to examine the acute effects of upper body BFR-BP exercise, utilising moderate to high loads (50-90% 1RM). It was hypothesised that wearing upper body BFR cuffs would increase BP barbell velocity.
METHODS: A well-trained male athlete (age, 46yrs; body mass, 105kg; height, 184cm; BP 1RM, 160kg) participated in a threeweek study. After 1RM testing and familiarisation in week 1, two experimental sessions were conducted in weeks 2 and 3, during which the athlete performed five sets of two repetitions, increasing loads from 50% to 90% 1RM with 10% incremental steps, and 3 minutes rest between sets. In week 2, the BP was performed without BFR cuffs (NOBFR), and in week 3 with BFR cuffs (BFR). The BFR cuff pressure was inflated to 80% of limb occlusion pressure of the upper limb and an intermittent inflation protocol was used. A linear transducer monitored mean barbell velocity (MV) for every repetition. Given the single-subject design, comparative data from a peer-reviewed study was utilised tocontextualise and enhance interpretation of the results. The reference study offered population-level data on similar BFR and NO-BFR conditions across the same incremental loads. Barbell velocity for the best repetition (MV) was analysed. Statistical analysis calculated effect sizes (ES), 95% confidence intervals (CI), and smallest worthwhile change (SWC), to assess the practical significance of differences between BFR and non BFR conditions.
RESULTS: Wearing BFR cuffs increased barbell velocity by 5.8% to 26.8% across all loads (Table 1). Small (60% 1RM, ES: 0.4; 70% 1RM, ES: 0.22) to moderate (50% 1RM, ES: 0.73, 80% 1RM, ES: 0.74; 90% 1RM, ES: 0.76) effects were observed for all the BFR interventions. Furthermore, the observed velocity differences across all loads exceeded the calculated SWC.
DISCUSSION: Wearing upper body BFR cuffs during moderate-to-high load BP increases barbell velocity in the participant. The ability to increase barbell velocity is advantageous as faster barbell velocities has been reported to improve strength and performance outcome over training with slower barbell velocities. This has positive implications for both welltrained and aging athletes, as training with an enhanced barbell velocity could improve strength outcomes, especially where strength gains plateau or decline with an increase in training and chronological age.
PRACTICAL APPLICATIONS: BFR training offers a practical methodology to enhance barbell velocity during moderate-to-high load RE. Coaches and athletes could use this approach longitudinally to maximise strength and power development, especially when traditional RE adaptations slow due to training experience or age-related decline.
Conflict of Interest: The BFR cuffs used in this study is a product that is sold by TheBFR.co of which the author is the owner. This potential conflict of interest has been disclosed and efforts have been made to ensure the research remains objective and unbiased
Molecular and statistical modeling of reduction peak potential and lipophilicity of platinum(IV) complexes
No full textMolecular and statistical modeling of reduction peak potential and lipophilicity of platinum(IV) complexes
No full textWe report the results of the quantitative structure–property relationship analysis of 31 Pt(IV) complexes, for three of which the synthesis is reported for the first time. The X-ray structural analysis of one complex of the series was performed to demonstrate that the PM6 semiempirical method satisfactorily reproduces key features of the geometry of the complexes investigated. Molecular properties extracted from such calculations were then used to construct models of experimental data such as electrochemical peak potentials (evaluated by cyclic voltammetry) and the octanol–water partition coefficient (evaluated by a reversed-phase high performance liquid chromatography method), which are key aspects in the design of such Pt(IV) complexes as potential anticancer prodrugs. Statistically accurate models for both properties were found using combinations of surface areas, orbital energies, dipole moments, and atomic partial charges. These models could form the basis of virtual screening of potential drug molecules, allowing the prediction of properties, closely related to the antiproliferative activity of Pt(IV) complexes, directly from calculated data
Antiproliferative Pt(IV) complexes: synthesis, biological activity, and quantitative structure–activity relationship modeling
No full textOrganometallic half-sandwich iridium anticancer complexes
No full textThe low-spin 5d6 IrIII organometallic half-sandwich complexes [(η5-Cpx)Ir(XY)Cl]0/+, Cpx = Cp*, tetramethyl(phenyl)cyclopentadienyl (Cpxph), or tetramethyl(biphenyl)cyclopentadienyl (Cpxbiph), XY = 1,10-phenanthroline (4−6), 2,2′-bipyridine (7−9), ethylenediamine (10 and 11), or picolinate (12−14), hydrolyze rapidly. Complexes with N,N-chelating ligands readily form adducts with 9-ethylguanine but not 9-ethyladenine; picolinate complexes bind to both purines. Cytotoxic potency toward A2780 human ovarian cancer cells increases with phenyl substitution on Cp*: Cpxbiph > Cpxph > Cp*; Cpxbiph complexes 6 and 9 have submicromolar activity. Guanine residues are preferential binding sites for 4−6 on plasmid DNA. Hydrophobicity (log P), cell and nucleus accumulation of Ir correlate with cytotoxicity, 6 > 5 > 4; they distribute similarly within cells. The ability to displace DNA intercalator ethidium bromide from DNA correlates with cytotoxicity and viscosity of Ir−DNA adducts. The hydrophobicity and intercalative ability of Cpxph and Cpxbiph make a major contribution to the anticancer potency of their IrIII complexes
