80 research outputs found
DS_775055 – Supplemental material for A Cytotoxic Three-Dimensional-Spheroid, High-Throughput Assay Using Patient-Derived Glioma Stem Cells
No full textSupplemental material, DS_775055 for A Cytotoxic Three-Dimensional-Spheroid, High-Throughput Assay Using Patient-Derived Glioma Stem Cells by Victor Quereda, Shurong Hou, Franck Madoux, Louis Scampavia, Timothy P. Spicer and Derek Duckett in SLAS Discovery</p
Supp_Mat_for_Development_of_Primary_Pancreatic_Organoid_Tumor_Models_for_HT_Pheno_Drug_Screening_by_Hue_et_al – Supplemental material for Advanced Development of Primary Pancreatic Organoid Tumor Models for High-Throughput Phenotypic Drug Screening
No full textSupplemental material, Supp_Mat_for_Development_of_Primary_Pancreatic_Organoid_Tumor_Models_for_HT_Pheno_Drug_Screening_by_Hue_et_al for Advanced Development of Primary Pancreatic Organoid Tumor Models for High-Throughput Phenotypic Drug Screening by Shurong Hou, Hervé Tiriac, Banu Priya Sridharan, Louis Scampavia, Franck Madoux, Jan Seldin, Glauco R. Souza, Donald Watson, David Tuveson, and Timothy P. Spicer in SLAS Discovery</p
DISC796410_Suppl – Supplemental material for Identification of Antimalarial Inhibitors Using Late-Stage Gametocytes in a Phenotypic Live/Dead Assay
No full textSupplemental material, DISC796410_Suppl for Identification of Antimalarial Inhibitors Using Late-Stage Gametocytes in a Phenotypic Live/Dead Assay by Timothy P. Spicer, Donald L. Gardiner, Frank J. Schoenen, Sudeshna Roy, Patrick R. Griffin, Peter Chase, Louis Scampavia, Peter Hodder and Katharine R. Trenholme in SLAS Discovery</p
Monitoring of HTS Compound Library Quality via a High-Resolution Image Acquisition and Processing Instrument
No full text This report presents the high-resolution image acquisition and processing instrument for compound management applications (HIAPI-CM). The HIAPI-CM combines imaging spectroscopy and machine-vision analysis to perform rapid assessment of high-throughput screening (HTS) compound library quality. It has been customized to detect and classify typical artifacts found in HTS compound library microtiter plates (MTPs). These artifacts include (1) insufficient volume of liquid compound sample, (2) compound precipitation, and (3) colored compounds that interfere with HTS assay detection format readout. The HIAPI-CM is also configured to automatically query and compare its analysis results to data stored in a LIMS or corporate database, aiding in the detection of compound registration errors. To demonstrate its capabilities, several compound plates ( n = 5760 wells total) containing different artifacts were measured via automated HIAPI-CM analysis, and results compared with those obtained by manual (visual) inspection. In all cases, the instrument demonstrated high fidelity (99.8% empty wells; 100.1% filled wells; 94.4% for partially filled wells; 94.0% for wells containing colored compounds), and in the case of precipitate detection, the HIAPI-CM results significantly exceeded the fidelity of visual observations (220.0%). As described, the HIAPI-CM allows for noninvasive, nondestructive MTP assessment with a diagnostic throughput of about 1 min per plate, reducing analytical expenses and improving the quality and stewardship of HTS compound libraries. </jats:p
IoT for Real-Time Measurement of High-Throughput Liquid Dispensing in Laboratory Environments
No full textUsing bead injection to model dispensing of 3-D multicellular spheroids into microtiter plates
No full textAuthor response
No full textImbalances in endoplasmic reticulum (ER) proteostasis are associated with etiologically-diverse degenerative diseases linked to excessive extracellular protein misfolding and aggregation. Reprogramming of the ER proteostasis environment through genetic activation of the Unfolded Protein Response (UPR)-associated transcription factor ATF6 attenuates secretion and extracellular aggregation of amyloidogenic proteins. Here, we employed a screening approach that included complementary arm-specific UPR reporters and medium-throughput transcriptional profiling to identify non-toxic small molecules that phenocopy the ATF6-mediated reprogramming of the ER proteostasis environment. The ER reprogramming afforded by our molecules requires activation of endogenous ATF6 and occurs independent of global ER stress. Furthermore, our molecules phenocopy the ability of genetic ATF6 activation to selectively reduce secretion and extracellular aggregation of amyloidogenic proteins. These results show that small molecule-dependent ER reprogramming, achieved through preferential activation of the ATF6 transcriptional program, is a promising strategy to ameliorate imbalances in ER function associated with degenerative protein aggregation diseases
FLOW INJECTION MONITORING OF ENZYME REACTIONS ON SOLID SURFACES
No full textOptosensing flow injection analysis may be used to monitor enzyme reactionson various types of
solid surfaces, e.g., cellulose, Teflon or polypropylene. The surfaces may bein the form ofa pad, a
membraneorparticles (in a column).
While the enzyme maybe in solution, advantages accrueif it is attached to the solid surface.
Traditional enzyme immobilization on surfaces involves chemical bonding reactions, but enzymes may be
directly adsorbed onto certain hydrophobic surfaces.
The principles of optosensing measurements are presented andapplicationsto cellulose pads and gas
barrier membranes for enzyme measurements are reviewed. Recent novel methodsfor derivatizing enzymes
for direct adsorption onto fluorocarbon membranesare presented. A new gas sensing (gas gap) membrane
is described in which the membraneacts as the actual sensor and notjust a barrier, suitable for optosensing
of monitoring enzyme reactions. Preliminary studies of the direct adsorption of urease enzyme onto the
membrane are presented.
Silica based C-18 particles in a microcolumn are used to adsorb an indicator and native urease
enzymé. Optosensing measurementsof urea by measuring transmittance through the particles are presented
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