146 research outputs found
The legacy of monastic hospitality : 1 the rule of Benedict and rise of western monastic hospitality
No full textIn the first of two articles about the founding father of hospitality, Kevin O'Gorman looks at St Benedict's rule and its context of in the monastic orders. Contemporary hospitality operators will find themselves in a very familiar world
Signal transduction protocols
No full textThe fascinating world of signal transduction is deeply, and clearly, investigated in this book edited by two leading scientists, Prof. Louis M. Luttrell (Dept. Medicine & Biochemistry & Molecular Biology, Medical University of South Carolina at Charleston) and Prof. Stephen S.G. Ferguson (Robarts Research Institute, The University of Western Ontario at London, Ontario). The editors were able to put together twentyfive chapters.....
Real-time BRET assays to measure G protein/effector interactions
No full textAdvances in imaging assays based on resonance energy transfer (RET) have made it possible to study protein/protein interactions in living cells under physiological conditions. It is now possible to measure the kinetics of changes in these interactions in response to ligand stimulation in real time. Here we describe protocols for these assays focusing on the basal and ligand-stimulated interaction between tagged G beta gamma subunits and adenylyl cyclase II. We describe relevant positive and negative controls and various experimental considerations for optimization of these experiments
Triggering and modulation of geyser eruptions in Yellowstone National Park by earthquakes, earth tides, and weather
No full textAuthor Posting. © American Geophysical Union, 2014. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Solid Earth 119 (2014): 1718–1737, doi:10.1002/2013JB010803.We analyze intervals between eruptions (IBEs) data acquired between 2001 and 2011 at Daisy and Old Faithful geysers in Yellowstone National Park. We focus our statistical analysis on the response of these geysers to stress perturbations from within the solid earth (earthquakes and earth tides) and from weather (air pressure and temperature, precipitation, and wind). We conclude that (1) the IBEs of these geysers are insensitive to periodic stresses induced by solid earth tides and barometric pressure variations; (2) Daisy (pool geyser) IBEs lengthen by evaporation and heat loss in response to large wind storms and cold air; and (3) Old Faithful (cone geyser) IBEs are not modulated by air temperature and pressure variations, wind, and precipitation, suggesting that the subsurface water column is decoupled from the atmosphere. Dynamic stress changes of 0.1−0.2 MPa resulting from the 2002 M-7.9 Denali, Alaska, earthquake surface waves caused a statistically significant shortening of Daisy geyser's IBEs. Stresses induced by other large global earthquakes during the study period were at least an order of magnitude smaller. In contrast, dynamic stresses of >0.5 MPa from three large regional earthquakes in 1959, 1975, and 1983 caused lengthening of Old Faithful's IBEs. We infer that most subannual geyser IBE variability is dominated by internal processes and interaction with other geysers. The results of this study provide quantitative bounds on the sensitivity of hydrothermal systems to external stress perturbations and have implications for studying the triggering and modulation of volcanic eruptions by external forces.K. Luttrell and S. Hurwitz
were supported by the USGS Volcano
Hazards Program, and Michael Manga
was supported by NSF grant
EAR1114184.2014-09-0
Heat Flux From a Vapor‐Dominated Hydrothermal Field Beneath Yellowstone Lake
No full textAuthor Posting. © American Geophysical Union, 2021. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Solid Earth 126(5),(2021): e2020JB021098, https://doi.org/10.1029/2020JB021098.We report results from 149 heat flux measurements made over an ∼2-year interval at sites in and around a vapor-dominated geothermal field located at water depths of ∼100–120 m in Yellowstone Lake, Wyoming. Measurements of both in situ temperature and thermal conductivity as a function of depth were made with a 1 m probe via a remotely operated vehicle, and are combined to compute the vertical conductive heat flux. Inside the ∼55.5 × 103 m2 bathymetric depression demarcating the vapor-dominated field, the median conductive flux is 13 W m−2, with a conductive output of 0.72 MW. Outside the thermal field, the median conductive flux is 3.5 W m−2. We observed 49 active vents inside the thermal field, with an estimated mass discharge rate of 56 kg s−1, a median exit-fluid temperature of 132°C, and a total heat output of 29 MW. We find evidence for relatively weak secondary convection with a total output of 0.09 MW in thermal area lake floor sediments. Our data indicate that vapor beneath the thermal field is trapped by a low-permeability cap at a temperature of ∼189°C and a depth of ∼15 m below the lake floor. The thermal output of the Deep Hole is among the highest of any vapor-dominated field in Yellowstone, due in part to the high boiling temperatures associated with the elevated lake floor pressures.This work was funded by U.S. National Science Foundation (NSF) grants EAR-1515283 to R. N. Harris and J. E. Favorito, EAR-1516361 to R. A. Sohn, and EAR-1514865 to K. M. Luttrell All work in Yellowstone National Park was completed under research permit (YELL-2018-SCI-7018) and the authors thank Annie Carlson from the Yellowstone Center for Resources for logistical help.2021-11-1
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