1,721,023 research outputs found
Isozyme diversity, RFLP of the rDNA and phylogenetic affinities among cultivated Lima beans, Phaseolus lunatus (Fabaceae). Plant. Systematics and Evolution.
No full textAnalisi comparativa sull’uso di tre classi di marcatori molecolari (RAPD, AFLP e SSR) nel rilevare polimorfismo in Cucumis melo L..
No full textMicropropagation of the early artichoke cultivar 'Violet du Provence'
Full text linkThe production of micropropagated transplants in early artichoke cuItivars is neglected by the loss of earliness and by the very low multiplication rate. The high concentration of hormones and nutrients and the high number of subcuItures are in contrast with the necessity to avoid genetic variations to which the loss of the earliness trait is attributed. In addition the contamination of the explants and the occurrence of oxidizating problems during initiation are frequentely repoted. Two pre-treatment protocol (Pl and P2) and four culture media were tested in initiation (El, E2, E3 and E4) and in multiplication (MI, M2, M3 and M4) phase in order to improve growth and multiplication rate of 'Violet du Provence' explants. Apices were treated with sodium hypocloride, before (Pl) and after (P2) the antioxidant solution. During initiation phase colture media were characterized by high (E2 and E3) or low (El and E4) hormonal level, while during muItiplication phase M2 was characterized by a higher content of hormones compared to MI, M3 and M4. Pl protocol determined a sensible reduction of the explant oxidation (11.6 vs 37.7%). The substrates E3 and E4 registered the highest growth values up to the last week; due to the rapid height increase observed in the last part of the initiation, the explants raised on El were 18% higher of that observed in E3 and E4 and 31% higher of those produced in E2. The M3 substrate determined the higher growth increase (208.9 mm), especially if compared to M4 (+57.2%). The number of shoots formed from every initial bud (multiplication index, 1M) was 4.5 in M3 with an average height of about 42 mm
Caratterizzazione di popolazioni locali di carosello e barattiere mediante l’uso di marcatori AFLP
No full textEvaluation of the potential of genomic tools and plant genes to mitigate the climate change impact and extremes on natural ecosystems and agriculture
No full textCAPS technology as a tool for the development of genic and functional markers: Study in peas
No full textDNA markers linked to economically important alleles are often used in plant breeding in order to replace/assist traditional phenotypic selection. Thanks to growing advances in agricultural genetics and genomics, it is possible today to develop genic markers and functional markers, the latter targeting polymorphisms, within a certain gene, directly responsible for the phenotype. It is known that allelic variation is commonly due to single nucleotide polymorphisms (SNPs) and small insertion/deletions (In/Del). In both cases restriction endonucleases recognition sites can be created or disrupted. Thus, cleaved amplified polymorphic sequence (CAPS) technology is particularly suitable for the development of genic and functional markers. In recent activities, we developed a series of CAPS and derived CAPS (dCAPS) functional markers which target all SNP mutations in a gene of cultivated pea (Pisum sativum L.) known to determine resistance to the powdery mildew pathogen Erysiphe pisi. Molecular markers other than CAPS, including those based on next generation sequencing methods, can be today used to target SNPs and In/Del polymorphisms. However, considering their ease of obtainment without the need of costly equipment, CAPS might be still the markers of choice for breeding programs which do not make use of genome-wide screenings and for species whose genetic/genomic information is limited
STUDIO DELLA DIVERSITÀ GENETICA TRA ECOTIPI DI CAROSELLO E BARATTIERE (CUCUMIS MELO L.).
No full textsupplemento al n.
L’analisi AFLP nell’identificazione varietale del carciofo (Cynara cardunculus L. var. scolymus).
No full textDNA occurrence in organic matter fractions isolated from amended, agricultural soils
No full textThe persistence of soil organic matter (SOM) constituents as a function of their recalcitrance has been recently questioned, with several papers showing the influence of the combined action of physical protection and chemical stabilization mechanisms as the main factors affecting SOM mean residence time. Using a physical fractionation method, SOM located between aggregates (FR), occluded within macro- (MA) and micro-aggregates (MI), and associated with the mineral fractions (MIN) were isolated from an agricultural soil differently amended (using compost, sewage sludge and biochar), and the occurrence of (total, bacterial and plant) DNA in these SOM pools was investigated. Following physical fractionation, total DNA (tDNA) was recovered from all SOM pools and from all treatments. Independently from the amendment, most tDNA accumulates in the FR fraction (30–70%), followed by the MIN pool (25–55%). The positive correlation between tDNA contents and C/N ratios (both tending to decrease following the order FR, MA and MI) observed for the light SOM fractions, together with the opposite pattern characterizing the heavy, MIN fraction (characterized by relatively high tDNA contents and low C/N ratios), suggests a different origin of the latter SOM pool, i.e., new molecules resulting from microbial transformations rather than highly degraded litter inputs. Therefore, tDNA may represent a promising proxy of organic matter dynamics in mineral soils. Finally, the MI fraction shows the highest number of microbial taxa and diversity, and seems to constitute a separate microbial niche in which different bacterial communities carry out their activity
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