196,284 research outputs found
BoLA class I polymorphism and in vitro immune response to M. bovis antigens
No full textFrom a sample of 119 Friesian calves, serologically typed for BoLA class I, 47 subjects were chosen expressing 9 different MHC types (A6, A6.9, A10, All, A14, A15, A30, W16, M103) with the same age and reared in the same farm conditions. The animals were s.c. injected with a water in oil suspension of killed M. bovis and the treatment was repeated two days later. Before the treatment and 21 days later, calves were bled and on PBM peripheral blood mononuclear leucocytes) were performed the following tests: 1. Lymphocyte Stimulation with bovine and avian PPDs (Purified protein derivative of Mycobacterium bovis and Mycobacterium, respectively). 2. Phagocytic activity towards M. bovis. 3. Class II molecules expression on cell surface. 4. Percentage of leucocyte populations and subpopulations. In the in vitro Lymphocyte Stimulation test, all the animals and classes were responders. Animals bearing A10 BoLA class I presented c.p.m. (counts per minute) and index values higher than the other cattle; these values were significantly positively related both to bovine and avian PPDs (P < .01). By variance analysis A14 BoLA type showed a slight positive significant correlation with more efficient phagocytic activity. BoLA class I type did not seem to significantly affect percentage of class II positive cells and leucocyte percentages on PBM
Valutazione col metodo ELISA della risposta umorale al M. bovis ucciso nel bovino in relazione al polimorfismo MHC di classe I
No full textDNA diagnostic tests for inherited diseases in pets in “Reproduction in companion exotic and laboratori animals
No full textIdentificazione di geni maggiori d’interesse economico (ETL) per la resistenza alle malattie
No full textDespite eradication programs, bovine tuberculosis is still a sanitary and economic problem in Italy. Genetic effect on immune response to M.bovis plays a central role to determine resistance or susceptibility to disease. The two genes so far identified as involved in immunity to TB are Major Histocompatibility Complex (MHC) and natural resistance associated macrophage protein 1 (NRAMP1). MHC polymorphism modulates antigen presentation and immune paths activation; NRAMP1 determines the ability of macrophages to control M. bovis infection. It has been demonstrated that NRAMP1 even influences the level of MHC class II expression. In this study we have analysed the polymorphisms and sequences of the MHC antigen-binding grove in calves immunised to M.bovis in relation to specific immune response. Moreover bovine NRAMP1 gene has been sequenced and analysed by SSCP technique and microsatellite analysis.
Parole chiave: Tubercolosi, Bovino, MHC, NRAMP1
BoLA class I polymorphism and in vitro immune response to M. bovis antigens : 1. Lymphocyte stimulation
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BoLA class I polymorphism and in vitro immune response to M. bovis antigens : 2. Phagocytic activity and class II expression
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