1,726,972 research outputs found
Architettura una Risorsa per Modernizzazione Convegni Convegno dell’Istituto Nazionale di Architettura, Roma, Auditorium della Confindustria, Gennaio 2000
No full textUn convegno internazionale organizzato dall'InArch con la conduzione scientifica di Antonino Saggio insieme a Massimo Locci, Luigi Prestinenza e M.assimo Bilò
Arlotti-Beccu-Desideri-Raimondo (by Massimo Locci)
No full textCurrently one of the most active offices in Rome, ABDR has seven major projects under way or at the point of completion. Massimo Locci explain the practice's work in its combination of a purist Modernist language aptitude for expressive informality
Arsenic contamination in the Baccu Locci mine watershed (Sardinia, Italy) after remediation
No full textThe old Pb-AsBaccu Locci mine represents the first and only case of mine site remediation in Sardinia, Italy. This paper reports preliminary results on the level of contamination in the Baccu Locci stream after remediation. In particular, distribution of dissolved As has not substantially changed compared to pre-remediation situation, showing an increasing trend along the stream and a concentration of about 400 μg/L at a distance of 5 km from the mine. Future monitoring of the Baccu Locci system will provide fundamental information on the effectiveness of remediation measures adopted and their applicability to other mine sites in Sardinia
Probing proteins in solution by 129Xe NMR spectroscopy
No full textThe interaction of xenon with different proteins in aqueous solution is investigated by (129)Xe NMR spectroscopy. Chemical shifts are measured in horse metmyoglobin, hen egg white lysozyme, and horse cytochrome c solutions as a function of xenon concentration. In these systems, xenon is in fast exchange between all possible environments. The results suggest that nonspecific interactions exist between xenon and the protein exteriors and the data are analyzed in term of parameters which characterize the protein surfaces. The experimental data for horse metmyoglobin are interpreted using a model in which xenon forms a 1:1 complex with the protein and the chemical shift of the complexed xenon is reported (Locci et al. Keystone Symposia "Frontiers of NMR in Molecular Biology VI", Jan. 9--15, 1999, Breckenridge, CO, Abstract E216, p. 53; Locci et al. XeMAT 2000 "Optical Polarization and Xenon NMR of Materials", June 28--30, 2000, Sestri Levante, Italy, p. 46).SCOPUS: ar.jinfo:eu-repo/semantics/publishe
Geochemistry of lead at the old mine area of Baccu Locci (South-East Sardinia, Italy)
No full textAbout a century of exploitation of the galena-arsenopyrite deposit of Baccu Locci in Sardinia (Italy) has caused a
severe, persistent arsenic contamination that extends downstream of the mine for several kilometres. Differently
from As, the aqueous contamination of lead is only localised in the upper part of the mine despite very high Pb
concentrations in geologic materials (waste rocks, tailings, stream sediments, soils) over the whole Baccu Locci
stream catchment. The determination of aqueous and solid Pb speciation in various environmental media of the
Baccu Locci system has pointed out that the peculiar geochemical behaviour of Pb is mainly due to (i) the short
residence time of dissolved Pb in surface and ground water under near-neutral pH conditions and (ii) the low
solubility of plumbojarosite that represents the main secondary Pb-bearing mineral in the Baccu Locci
environment
Stream water chemistry in the arsenic-contaminated Baccu Locci mine watershed (Sardinia, Italy) after remediation
No full textThe abandoned Pb-As Baccu Locci mine represents the first and only case of mine site remediation in Sardinia, Italy. Arsenic is the most relevant environmental concern in the Baccu Locci stream watershed, with concentrations in surface waters up to and sometimes over 1 mg/L. The main remediation action consisted in creation of a "storage site", for the collection of contaminated materials from different waste-rock dumps and most of tailings piles occurring along the Baccu Locci stream. This paper reports preliminary results on the level of contamination in the Baccu Locci stream after the completion of remediation measures. Post-remediation stream water chemistry has not substantially changed compared to the pre-remediation situation. In particular, dissolved As maintains an increasing trend along the Baccu Locci stream, with a concentration of about 400 μg/L measured at a distance of 7 km from the storage site. Future monitoring will provide fundamental information on the effectiveness of remediation actions conducted and their applicability to other mine sites in Sardinia. At the stage of mine site characterisation of future remediation plans, it is recommended to pay more attention to the understanding of mineralogical and geochemical processes responsible for pollution. Moreover, mixing of materials with different composition and reactivity in a storage site should require careful consideration and long-term leaching tests
Characterization of invariant NKT cell phenotype and function in Wiskott-Aldrich Syndrome (WAS)
No full textWASp è una proteina che regola il rimodellamento dell’actina del citoscheletro nelle cellule ematopoietiche. Mutazioni nel gene che codifica per WASp (WAS) causano la Sindrome di Wiskott-Aldrich (WAS). Sebbene WASp sia coinvolto in svariate funzioni delle cellule del sistema immunitario, il suo ruolo nei linfociti invarianti NKT (iNKT) non è mai stato investigato. Difetti delle cellule iNKT potrebbero infatti contribuire allo sviluppo di alcune caratteristiche dei pazienti WAS quali le infezioni ricorrenti e l’ alta incidenza tumorale. Infatti il nostro studio ha rivelato una profonda riduzione numerica dei linfociti iNKT periferici nei pazienti WAS, direttamente correlata con la severità del fenotipo clinico dei pazienti. Per definire ulteriormente il fenotipo delle cellule iNKT prive di WASp, abbiamo esteso l’analisi ai topi was-/-. Le cellule iNKT sono significativamente ridotte anche nel timo e negli organi linfoidi periferici dei topi was-/- rispetto ai controlli wt. Inoltre l’analisi dello sviluppo delle cellule was-/- iNKT cell ha messo in luce un completo blocco maturativo allo stadio intermedio CD44+NK1.1-. In particolare, la generazione di chimere di midollo osseo, ha dimostrato un difetto maturativo intrinseco delle cellule was-/- iNKT. L’assenza di WASp non altera la stimolazione indotta dall’IL-15, che è importante nello sviluppo delle cellule iNKT. Contrariamente, WASp è coinvolto nel controllo della proliferazione omeostatica di questa tipologia cellulare. Le cellule iNKT prive di WASp presentano anche un difetto funzionale, come messo in evidenza dalla ridotta secrezione di IL-4 e IFN-γ dopo la loro attivazione in vivo. In aggiunta, saggi funzionali condotti in vitro, suggeriscono che il difetto funzionale delle cellule iNKT prive di WASp sia mediato dal TCR e che la ridotta produzione di IL-4 sia causata da un difetto funzionale intrinseco alle cellule iNKT, mentre la minor produzione di IFN-γ sembra derivare da un’interazione non efficiente tra le cellule was-/- iNKT cells e le cellule dendritiche was-/-. Nel loro insieme questi risultati dimostrano il ruolo rilevante di WASp nell’integrare segnali critici per lo sviluppo e la funzione delle cellule iNKT, e suggeriscono che i difetti di questa popolazione linfocitaria possano contribuire alla patologia della Sindrome di Wiskott-Aldrich.WAS protein (WASp) is a key regulator of actin cytoskeleton in hematopoietic cells. Mutations of WAS gene cause the Wiskott-Aldrich Syndrome (WAS). Although WASp is involved in various immune cell functions, its role in invariant NKT cells (iNKT) has never been investigated. Defects of iNKT cells could contribute to the pathogenesis of several WAS features, such as recurrent infections and high tumor incidence. Indeed, we found a profound reduction of circulating iNKT cells in WAS patients, directly correlating with the severity of clinical phenotype. To better characterize iNKT cell defect in the absence of WASp, we analyzed was-/- mice. iNKT cell number is significantly reduced in thymus and periphery of was-/- mice as compared to wt controls. Moreover analysis of was-/- iNKT cell maturation reveals a complete arrest at the CD44+NK1.1- intermediate stage. Notably, generation of BM chimeras demonstrated a was-/- iNKT cell autonomous developmental defect. The lack of WASp does not affect IL-15 signaling, which is important in iNKT cell development. Conversely WASp is required for the control of homeostatic proliferation. was-/- iNKT cells are also functionally impaired, as suggested by the lower expansion and reduced secretion of IL-4 and IFN-γ upon in vivo activation. Furthermore, in vitro assays suggest that the functional defect of WASp null iNKT cells is TCR-mediated and indicated that the defective IL-4 production is due to a was-/- iNKT cell autonomous defect, whereas the lower IFN-γ production is caused by an inefficient crosstalk between was-/- iNKT cells and was-/- DC. Altogether, these results demonstrate the relevance of WASp in integrating signals critical for development and functional differentiation of iNKT cells, and suggest that defects in these cells may play a role in WAS pathology
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