1,723,043 research outputs found
U937 JV9298
No full textLegionella pneumophila JV9298 DotF-GFP DotB E191K<strong>Tilt Series Date:</strong> 2020-12-12</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -6.0 μm, magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation, data collection & processing: Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 1h infection, grids coated with 25ug/mL vitronectin</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 gold finder grid, 100% humidity, 22deg C, manual blotting</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-21</p>U937_9298_tomo_17_bin2_SIRT_1k.rec, Reconstruction (Pixel Size 1.06 nm), 4.1 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-21/3dimage_123648/U937_9298_tomo_17_bin2_SIRT_1k.rec"
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U937_9298_tomo_17_bin2.st, Tilt Series (Pixel Size 0.53 nm), 636.4 MB
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U937 JV9082
No full textLegionella pneumophila JV9298 DotF-GFP<strong>Tilt Series Date:</strong> 2020-12-12</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -6.0 μm, magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation, data collection & processing: Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 1h infection, grids coated with 25ug/mL vitronectin</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 gold finder grid, 100% humidity, 22deg C, manual blotting</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-6</p>U937_9082_tomo_6_bin2.st, Tilt Series (Pixel Size 0.53 nm), 330.0 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-6/rawdata/U937_9082_tomo_6_bin2.st"
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U937 JV9298
No full textLegionella pneumophila JV9298 DotF-GFP DotB E191K<strong>Tilt Series Date:</strong> 2020-12-12</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -6.0 μm, magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation, data collection & processing: Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 1h infection, grids coated with 25ug/mL vitronectin</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 gold finder grid, 100% humidity, 22deg C, manual blotting</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-16</p>U937_9298_tomo_12_bin2_SIRT_1k.rec, Reconstruction (Pixel Size 1.06 nm), 4.1 GB
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U937_9298_tomo_12_bin2.st, Tilt Series (Pixel Size 0.53 nm), 601.0 MB
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U937 JV9298
No full textLegionella pneumophila JV9298 DotF-GFP DotB E191K<strong>Tilt Series Date:</strong> 2020-12-12</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -6.0 μm, magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation, data collection & processing: Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 1h infection, grids coated with 25ug/mL vitronectin</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 gold finder grid, 100% humidity, 22deg C, manual blotting</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-20</p>U937_9298_tomo_16_bin2_SIRT_1k.rec, Reconstruction (Pixel Size 1.06 nm), 4.1 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-20/3dimage_123646/U937_9298_tomo_16_bin2_SIRT_1k.rec"
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U937_9298_tomo_16_bin2.st, Tilt Series (Pixel Size 0.53 nm), 483.2 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-20/rawdata/U937_9298_tomo_16_bin2.st"
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U937 JV9298
No full textLegionella pneumophila JV9298 DotF-GFP DotB E191K<strong>Tilt Series Date:</strong> 2020-12-12</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -6.0 μm, magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation, data collection & processing: Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 1h infection, grids coated with 25ug/mL vitronectin</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 gold finder grid, 100% humidity, 22deg C, manual blotting</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-17</p>U937_9298_tomo_13_bin2_SIRT_1k.rec, Reconstruction (Pixel Size 1.06 nm), 4.1 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-17/3dimage_123640/U937_9298_tomo_13_bin2_SIRT_1k.rec"
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U937_9298_tomo_13_bin2.st, Tilt Series (Pixel Size 0.53 nm), 624.6 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-17/rawdata/U937_9298_tomo_13_bin2.st"
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U937 JV9298
No full textLegionella pneumophila JV9298 DotF-GFP DotB E191K<strong>Tilt Series Date:</strong> 2020-12-12</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -6.0 μm, magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation, data collection & processing: Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 1h infection, grids coated with 25ug/mL vitronectin</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 gold finder grid, 100% humidity, 22deg C, manual blotting</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-7</p>U937_9298_tomo_1_bin2_SIRT_1k.rec, Reconstruction (Pixel Size 1.06 nm), 4.1 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-7/3dimage_123622/U937_9298_tomo_1_bin2_SIRT_1k.rec"
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U937_9298_tomo_1_bin2.st, Tilt Series (Pixel Size 0.53 nm), 377.1 MB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-7/rawdata/U937_9298_tomo_1_bin2.st"
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U937 JV9298
No full textLegionella pneumophila JV9298 DotF-GFP DotB E191K<strong>Tilt Series Date:</strong> 2020-12-12</p>
<strong>Data Taken By:</strong> Yuxi Liu</p>
<strong>Species / Specimen:</strong> Legionella pneumophila</p>
<strong>Strain:</strong> Philadelphia-1_Lp02</p>
<strong>Tilt Series Settings:</strong> Single Axis, tilt range: (-60.0°, 60.0°), step: 2°, constant angular increment, dosage: 120.0 eV/Ų, defocus: -6.0 μm, magnification: 33000x. </p>
<strong>Acquisition Software:</strong> Serial EM</p>
<strong>Upload Method:</strong> rundir</p>
<strong>Processing Software Used:</strong> imod, tomo3d</p>
<strong>Collaborators and Roles:</strong> Sample preparation, data collection & processing: Yuxi Liu</p>
<strong>Purification / Growth Conditions / Treatment:</strong> 1h infection, grids coated with 25ug/mL vitronectin</p>
<strong>Sample Preparation:</strong> Quantifoil R2/2 gold finder grid, 100% humidity, 22deg C, manual blotting</p>Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-10</p>U937_9298_tomo_4_bin2_SIRT_1k.rec, Reconstruction (Pixel Size 1.06 nm), 4.1 GB
<a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/ylx2020-12-12-10/3dimage_123628/U937_9298_tomo_4_bin2_SIRT_1k.rec"
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U937_9298_tomo_4_bin2.st, Tilt Series (Pixel Size 0.53 nm), 294.6 MB
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