14 research outputs found
Xuanxue’s 玄學 ongoing evolution: Thinking with Wang Bi’s name-actuality dialectic
No full textHow should we conceptualise a philosophical tradition retrospectively defined and continuously shaped by contemporary scholarship? This paper addresses this philosophy-of-historiography question by focusing on Xuanxue, a significant yet underdeveloped domain of Chinese philosophy centered on the corpus of the Wei-Jin (魏晉) Period (220–589 CE) and beyond. Scholarly conceptions of Xuanxue have long been bound by entrenched themes, the ‘Daoist’ label, and rigid periodisation. In contrast, this paper situates Xuanxue within both its historical milieu and the context of ongoing modern interpretations. Drawing heuristically from Wang Bi’s (王弼, 226–249 CE) conceptual interdependence between Name (ming 名) and Actual Situation (shi 實), I propose a new method (HPE) for evaluating Xuanxue conceptions with three criteria: Historical embeddedness, Plurality, and Evolvingness. This research thus offers a philosophically reflexive model for Xuanxue studies and the broader field of cross-cultural philosophy, demonstrating how ancient traditions can be engaged as dynamic intellectual projects
An Onto-Hermeneutic Analysis of “Jinsheng Yuzhen” in the Warring States Period
Full text linkThe concept of “jinsheng yuzhen” appears in both the Wuxing and Mencius, but the “benti” of Confucianism in the Warring States period contained in this concept has not been thoroughly understood. This paper incorporates and analyzes “onto-Hermeneutics”, applying it to concepts and conceptual histories to clarify the debates around “jinsheng yuzhen” and explore its broader hermeneutic space. The concept of “jinsheng yuzhen” aims to conclude, unify, and transcend “jinsheng” through “yuzhen”, which means that both Wuxing and Mencius integrate pre-Qin Confucian virtues to interpret the “Way of Heaven”. In addition, “jinsheng yuzhen” begins with the “jinsheng” but the “yuzhen” does not have a specific endpoint. This metaphor indicates that the Way of Heaven is embodied by human beings as the self-awareness, cultivation, and practice of the inherent “benevolence” of the innate goodness of nature. Because of human creativity and the infinite nature of experiential reality, this process ultimately leads to the boundless unfolding, enrichment, and generation of the Way of Heaven
Correction: Assimilable organic carbon (AOC) determination using GFP-tagged Pseudomonas fluorescens P-17 in water by flow cytometry.
No full text[This corrects the article DOI: 10.1371/journal.pone.0199193.]
Assimilable organic carbon (AOC) determination using GFP-tagged Pseudomonas fluorescens P-17 in water by flow cytometry.
No full textOne of the newly developed methods for Assimilable organic carbon (AOC) determination is leveraged on the cell enumeration by flow cytometry (FC) which could provide a rapid and automated solution for AOC measurement. However, cell samples staining with fluorescence dye is indispensable to reduce background and machine noise. This step would bring additional cost and time consuming for this method. In this study, a green fluorescence protein (GFP) tagged strain derived of AOC testing strain Pseudomonas fluorescens P-17 (GFP-P17) was generated using Tn5 transposon mutagenesis. Continuous culture of this mutant GFP-P17 showed stable expression of eGFP signal detected by flow cytometry without staining step. In addition, this GFP-P17 strain displayed faster growth rate and had a wider range of carbon substrate utilization patterns as compared with P17 wild-type. With this strain, the capability of a new FC method with no dye staining was explored in standard acetate solution, which suggests linear correlation of counts with acetate carbon concentration. Furthermore, this FC method with GFP-P17 strain is applicable in monitoring GAC/BAC efficiency and condition as similar trends of AOC level in water treatment process were measured by both FC method and conventional spread plating count method. Therefore, this fast and easily applicable GFP-P17 based FC method could serve as a tool for routine microbiological drinking water monitoring
Batch growth curves of GFP-P17 and P17.
No full textGrowth conditions: 100 µg/L of acetate carbon with supplement of mineral salts. Error bars indicate standard deviation on triplicate samples.</p
Comparison of carbon substrate utilization pattern of GFP-P17 and P17.
No full textComparison of carbon substrate utilization pattern of GFP-P17 and P17.</p
Cell counts for growth of GFP-P17 cells on a defined carbon source (sodium acetate).
No full textCell counts for growth of GFP-P17 cells on a defined carbon source (sodium acetate).</p
Dot plots for GFP-P17 cells grown on water of 100 µg/L of acetate carbon with supplement of mineral salts.
No full textEnumeration gates are drawn to distinguish cells from background. Data was recorded in one minute. Cell counts: Day 0, 457/mL; Day 2, 3647/mL; Day 3, 80737/mL; Day 4, 61508/mL.</p
Representative fluorescence histogram data of GFP-P17 and P17 in water.
No full textThe x-axis shows green (FITC) fluorescence intensity, and the y-axis shows the number of events recorded for a corresponding fluorescence intensity. 1, water sample without cells; 2, water sample with P17; 3, water sample with GFP-P17.</p
Schematic presentation of our method compared to other methods.
No full textA. Conventional method; B, Flow cytometric method with cell staining. C, Our flow cytometric method without cell staining.</p
