8,285 research outputs found

    Monitoring Cl- movement in single cells exposed to hypotonic solution

    No full text
    Self-referencing ion--selective electrodes (ISEs), made with Chloride Ionophore I-Cocktail A (Fluka), were positioned 1-3 microm from human embryonic kidney cells (tsA201a) and used to record chloride flux during a sustained hyposmotic challenge. The ISE response was close to Nernstian when comparing potentials (VN) measured in 100 and 10 mM NaCl (deltaVN = 57 +/- 2 mV), but was slightly greater than ideal when comparing 1 and 10 mM NaCl (deltaVN = 70 +/- 3 mV). The response was also linear in the presence of 1 mM glutamate, gluconate, or acetate, 10 microM tamoxifen, or 0.1, 1, or 10 mM HEPES at pH 7.0. The ISE was approximately 3 orders of magnitude more selective for Cl- over glutamate or gluconate but less than 2 orders of magnitude move selective for Clover bicarbonate, acetate, citrate or thiosulfate. As a result this ISE is best described as an anion sensor. The ISE was 'poisoned' by 50 microM 5-nitro-2-(3phenylpropyl-amino)-benzoic acid (NPPB), but not by tamoxifen. An outward anion efflux was recorded from cells challenged with hypotonic (250 +/- 5 mOsm) solution. The increase in efflux peaked 7-8 min before decreasing, consistent with regulatory volume decreases observed in separate experiments using a similar osmotic protocol. This anion efflux was blocked by 10 microM tamoxifen. These results establish the feasibility of using the modulation of electrochemical, anion-selective, electrodes to monitor anions and, in this case, chloride movement during volume regulatory events. The approach provides a real-time measure of anion movement during regulated volume decrease at the single-cell leve

    Siles, S.S., Letter to Mary Knight, September 20, 1863

    Full text link
    Stiles, S.S., Letter to Mary Knight, September 20, 1863. MS/52 William Knight Civil War Letters, Box 1, Folder 3. Valdosta State University Archives and Special Collections. 1 PDF and 2 scans. Includes transcript.The letter, dated September 20, 1863, is from Camp Lee and addressed to Mary. The author, identified as S.S. Siles, expresses relief that William has returned home and hopes for his quick recovery. Siles mentions the difficulties faced by women who visit camps to bring their sick husbands home, noting that officers rarely pay them any attention. Siles laments the poor condition of the tents, which are old and worn, failing to provide adequate shelter from rain. He mentions that the Quarter Master tried but failed to secure new tents in Savannah. The letter also touches on the precarious state of the Confederate army, suggesting that its future may hinge on an upcoming battle in Northern Georgia. Siles fears that a defeat could lead to mass desertions. The letter describes the men as being very sickly, attributing this to their poor diet of only beef and cornmeal. Siles himself is not in good health, suffering from chest pains and fevers, but continues to perform his duties as an acting Orderly Sergeant. He urges Mary to tell William to write to him and to keep him updated with news

    Declaration of Intention for Orazio Colletto

    No full text
    Declaration of Intention to become a citizen of the United States, as filled out and signed by Orazio Colletto. Applicant lists himself as a 58 year old farmer residing in Hammonton, New Jersey, born in Bordenaro, Italy on 17 January 1857, who sailed on the US bound vessel S.S. Prince. Declaration submitted and sworn June 3, 1915

    ZAVIČAJNO OPREDJELJENJE S.S. KRANJČEVIĆA

    Full text link
    Hrvatski pjesnik S.S. Kranjčević (1865-1908), iako stvaralac nacionalnih i univerzalnih dimenzija, u određenom broju svojih pjesama iskazivao je na osebujan način svoje zavičajno određenje. Te njegove pjesme prožete snažnim emocijskim nabojima predočavaju njegovo viđenje ondašnje hrvatske nacionalne sudbine na prostoru njegova rodnog grada Senja u kontekstu uskočke tradicije koja je Senjane i Hrvate toliko nadahnjivala slobodarstvom i otporom tuđinskim porobljivačkim nasrtajima.The Croatian poet S.S. Kranjčević (1865-1908), though an author of national and universal dimensions, expressed his own native disposition in a number of his poems. Those of his poems, which are imbued with a strong emotional charge, present his own seeing of the Croatian national desetiny of the time in the area of his native town of Senj, and in the context of the Uskok (fighters against the Venetians and Turks) tradition which so much insipred the inhabitants (of Senj) and Croats in general with the love of freedom and resistance to the foreign enslaving assaults

    Strong convergence theorem of a hybrid projection algorithm for a family of quasi-φ-asymptotically nonexpansive mappings

    No full text
    Tyt. z nagłówka.Pozostali autorzy art.: S.S. Chang, M. Liu, J.A. Liu.Bibliogr. s. 348.Dostępny również w formie drukowanej.ABSTRACT: The main purpose of this paper is by using a new hybrid projection iterative algorithm to prove some strong convergence theorems for a family of quasi-φ-asymptotically nonexpansive mappings. The results presented in the paper improve and extend the corresponding results announced by some authors. KEYWORDS: quasi-phi-asymptotically nonexpansive mapping, asymptotically regular mapping, hybrid projection iterative algorithm, strong convergence theorem

    Real-space Manifestations of Bottlenecks in Turbulence Spectra

    No full text
    An energy-spectrum bottleneck, a bump in the turbulence spectrum between the inertial and dissipation ranges, is shown to occur in the non-turbulent, one-dimensional, hyperviscous Burgers equation and found to be the Fourier-space signature of oscillations in the real-space velocity, which are explained by boundary-layer-expansion techniques. Pseudospectral simulations are used to show that such oscillations occur in velocity correlation functions in one- and three-dimensional hyperviscous hydrodynamical equations that display genuine turbulence

    Bending dynamics of semi-flexible particles in turbulent flows

    No full text
    We study the Lagrangian dynamics of semi-flexible particles in laminar as well as in homogeneous and isotropic turbulent flows by means of analytically solvable stochastic models and direct numerical simulations. The statistics of the bending angle is qualitatively different in laminar and turbulent flows and exhibits a strong dependence on the topology of the velocity field. In particular, in two-dimensional turbulence, particles are either found in a fully extended or in a fully folded configuration; in three dimensions, the predominant configuration is the fully extended one

    On the approximation problem of common fixed points for a finite family of non-self asymptotically quasi-nonexpansive-type mappings in Banach spaces

    No full text
    AbstractThe purpose of this paper is to introduce the concept of non-self asymptotically quasi-nonexpansive-type mappings and to construct a iterative sequence to converge to a common fixed point for a finite family of non-self asymptotically quasi-nonexpansive-type mappings in Banach spaces. The results presented in this paper improve and extend the corresponding results in Alber, Chidume and Zegeye [Ya.I. Alber, C.E. Chidume, H. Zegeye, Approximating of total asymptotically nonexpansive mappings, Fixed Point Theory and Applications (2006) 1–20. Article ID10673], Ghosh and Debnath [M.K. Ghosh, L. Debnath, Convergence of Ishikawa iterates of quasi-nonexpansive mappings, Journal of Mathematical Analysis and Applications 207 (1997) 96–103], Liu [Q.H. Liu, Iterative sequences for asymptotically quasi-nonexpansive type mappings, Journal of Mathematical Analysis and Applications 259 (2001) 1–37; Q.H. Liu, Iterative sequences for asymptotically quasi-nonexpansive mappings with error member, Journal of Mathematical Analysis and Applications 259 (2001) 18–24; Q.H. Liu, Iteration sequences for asymptotically quasi-nonexpansive mapping with an error member of uniform convex Banach space, Journal of Mathematical Analysis and Applications 266 (2002) 468–471], Petryshyn [W.V. Petryshyn, T.E. Williamson Jr., Strong and weak convergence of the sequence of successive approximations for quasi-nonexpansive mappings, Journal of Mathematical Analysis and Applications 43 (1973) 459–497], Quan and Chang [J. Quan, S.S. Chang, X.J. Long, Approximation common fixed point of asymptotically quasi-nonexpansive type mappings by the finite steps iterative sequences, Fixed Point Theory and Applications V (2006) 1–38. Article ID 70830], Shahzad and Udomene [N. Shahzad, A. Udomene, Approximating common fixed point of two asymptotically quasi-nonexpansive mappings in Banach spaces, Fixed Point Theory and Applications (2006) 1–10. Article ID 18909] Xu [B.L. Xu, M.A. Noor, Fixed-point iterations for asymptotically nonexpansive mappings in Banach spaces, Journal of Mathematical Analysis and Applications 267 (2002) 444–453], Zhang [S.S. Zhang, Iterative approximation problem of fixed points for asymptotically nonexpansive mappings in Banach spaces, Acta Mathematicae Applicatae Sinica 24 (2001) 236–241] and Zhou and Chang [Y.Y. Zhou, S.S. Chang, Convergence of implicit iteration process for a finite family of asymptotically nonexpansive mappings in Banach spaces, Numerical Functional Analysis and Optimization 23 (2002) 911–921]
    corecore