1,721,037 research outputs found
Atrizine And Malathion Shorten The Maturation Process of Xenopus Leaves Oocytes And Have An Adverse Effect On Early Embryo Development
No full textThe use of pesticides has a negative impact on the environment. Amphibians have long been regarded as indica- tor species to pollutants due to their permeable skin and sensitivity to the environment. Studies have shown that population declines of some amphibians are directly linked with exposure to agricultural contaminants. In the past, much of the studies have focused on the toxic effect of contaminants on larvae (tadpoles), juvenile and adult frogs. However, due to the nature of their life cycle, amphibian eggs and early embryos are especially sus- ceptible to the contaminants, and any alteration during the early reproductive stagesmay have a profound effect on the health and population of amphibians. In this study, we analyzed the effect of atrazine andmalathion, two commonly used pesticides, on Xenopus laevis oocyte maturation and early embryogenesis.We found that both atrazine andmalathion shortened the frog oocyte maturation process and resulted in reduced Emi2 levels at cy- tostatic factor-mediatedmetaphase arrest, and a high level of Emi2 is critically important for oocyte maturation. Furthermore, frog embryos fertilized under the influence of atrazine and/ormalathion displayed a higher rate of abnormal division that eventually led to embryo death during early embryogenesis
Analgesic diterpenoids with diverse carbon skeletons from the leaves of Rhododendron auriculatum
No full textSun, Na, Feng, Yuanyuan, Zhang, Qihua, Liu, Junjun, Zhou, Haofeng, Zhang, Hanqi, Zheng, Guijuan, Zhou, Junfei, Yao, Guangmin (2019): Analgesic diterpenoids with diverse carbon skeletons from the leaves of Rhododendron auriculatum. Phytochemistry 168: 1-11, DOI: 10.1016/j.phytochem.2019.112113, URL: http://dx.doi.org/10.1016/j.phytochem.2019.11211
Fig. 8 in Terpene Shikimate conjugated meroterpenoids from the endophytic fungus Guignardia mangiferae
No full textFig. 8. ΔδH(S-R) value of MTPA esters of 6 in CDCl3.Published as part of Chen, Keliang, Chen, Chunmei, Liu, Xiulan, Sun, Weiguang, Deng, Yanfang, Liu, Junjun, Wang, Jianping, Luo, Zengwei, Zhu, Hucheng & Zhang, Yonghui, 2021, Terpene Shikimate conjugated meroterpenoids from the endophytic fungus Guignardia mangiferae, pp. 1-9 in Phytochemistry (112860) 190 on page 6, DOI: 10.1016/j.phytochem.2021.112860, http://zenodo.org/record/825739
Five undescribed steroids from Talaromyces stipitatus and their cytotoxic activities against hepatoma cell lines
No full textZhang, Mi, Deng, Yanfang, Liu, Fei, Zheng, Meijia, Liang, Yu, Sun, Weiguang, Li, Qin, Li, Xiao-Nian, Qi, Changxing, Liu, Junjun, Chen, Chunmei, Zhu, Hucheng, Zhang, Yonghui (2021): Five undescribed steroids from Talaromyces stipitatus and their cytotoxic activities against hepatoma cell lines. Phytochemistry (112816) 189: 1-10, DOI: 10.1016/j.phytochem.2021.112816, URL: http://dx.doi.org/10.1016/j.phytochem.2021.11281
Fig. 2. Key 1H–1H in Terpene Shikimate conjugated meroterpenoids from the endophytic fungus Guignardia mangiferae
No full textFig. 2. Key 1H–1H COSY and HMBC correlations of compounds 1, 3, 6, and 9.Published as part of Chen, Keliang, Chen, Chunmei, Liu, Xiulan, Sun, Weiguang, Deng, Yanfang, Liu, Junjun, Wang, Jianping, Luo, Zengwei, Zhu, Hucheng & Zhang, Yonghui, 2021, Terpene Shikimate conjugated meroterpenoids from the endophytic fungus Guignardia mangiferae, pp. 1-9 in Phytochemistry (112860) 190 on page 4, DOI: 10.1016/j.phytochem.2021.112860, http://zenodo.org/record/825739
Fig. 3 in Structurally diverse and bioactive alkaloids from an insect-derived fungus Neosartorya fischeri
No full textFig. 3. Selected NOESY/ROESY correlations of compounds 1–4, 8, 12, and 16.Published as part of Lin, Shuang, He, Yan, Li, Fengli, Yang, Beiye, Liu, Mengting, Zhang, Sitian, Liu, Junjun, Li, Huaqiang, Qi, Changxing, Wang, Jianping, Hu, Zhengxi & Zhang, Yonghui, 2020, Structurally diverse and bioactive alkaloids from an insect-derived fungus Neosartorya fischeri, pp. 1-10 in Phytochemistry (112374) 175 on page 4, DOI: 10.1016/j.phytochem.2020.112374, http://zenodo.org/record/829536
Fig. 4 in Five undescribed steroids from Talaromyces stipitatus and their cytotoxic activities against hepatoma cell lines
No full textFig. 4. Experimental ECD curve of 5 and calculated ECD curves of 5 and ent-5.Published as part of Zhang, Mi, Deng, Yanfang, Liu, Fei, Zheng, Meijia, Liang, Yu, Sun, Weiguang, Li, Qin, Li, Xiao-Nian, Qi, Changxing, Liu, Junjun, Chen, Chunmei, Zhu, Hucheng & Zhang, Yonghui, 2021, Five undescribed steroids from Talaromyces stipitatus and their cytotoxic activities against hepatoma cell lines, pp. 1-10 in Phytochemistry (112816) 189 on page 4, DOI: 10.1016/j.phytochem.2021.112816, http://zenodo.org/record/825957
Fig. 3 in Five undescribed steroids from Talaromyces stipitatus and their cytotoxic activities against hepatoma cell lines
No full textFig. 3. The NOESY correlations of compounds 1, 2, and 4–6.Published as part of Zhang, Mi, Deng, Yanfang, Liu, Fei, Zheng, Meijia, Liang, Yu, Sun, Weiguang, Li, Qin, Li, Xiao-Nian, Qi, Changxing, Liu, Junjun, Chen, Chunmei, Zhu, Hucheng & Zhang, Yonghui, 2021, Five undescribed steroids from Talaromyces stipitatus and their cytotoxic activities against hepatoma cell lines, pp. 1-10 in Phytochemistry (112816) 189 on page 3, DOI: 10.1016/j.phytochem.2021.112816, http://zenodo.org/record/825957
"Selection of DNA aptamers for rapid and specific detection of Listeria monocytogenes in food products"
No full textListeria monocytogenes (L. monocytogenes) is a facultative anaerobic bacterium, which causes the infection listeriosis and is one of the most virulent foodborne pathogens. Listeriosis predominately affects pregnant women, newborns, adults with weak immune system such as the elderly. It is estimated that 1,600 people get listeriosis and about 260 die each year. It is therefore very important to develop rapid and effective methods for L. monocytogenes test to protect human health and avoid costly food product recalls. L. monocytogenes in food and environmental samples are traditionally detected by cultural enrichment and selective/differential plating followed by biochemical, serological, and/or molecular identification. These methods are labor intensive and tedious, and usually take 2-3 days to confirm a negative and up to a week or more for a positive. Antibody-based immunoassays are fast, reproducible, and less sensitive to food interference therefore are widely used in L. monocytogenes detection. However, due to the nature of antibodies, their binding affinities and quality may vary. Recently, aptamers have emerged as a good alternative to antibodies in pathogen detection. Aptamers are stable short single-stranded oligonucleotides or peptides that bind to targets with high affinity and specificity. As they are produced by in vitro synthesis, their quality is more consistent. By using whole cell L. monocytogenes as target, single-stranded DNA aptamers can be selected. The ultimate goal of the project is to develop an aptamer-based rapid and specific L. monocytogenes detection method for food safety test.Oral Presentatio
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