491 research outputs found
Crovidisin, a collagen-binding protein isolated from snake venom of Crotalus viridis, prevents platelet-collagen interaction.
No full textMeasurement of glycoprotein IIb/IIIa blockade by flow cytometry with fluorescein isothiocyanate-conjugated crotavirin, a member of disintegrins.
No full textTrimucytin, a collagen-like snakevenom protein, activates platelets independent of I-domain within subunit of α2β1 integrin.
No full textInhibition of RPE cell-mediated matrix adhesion and collagen gel contraction by crovidisin, a collagen-binding snake venom protein.
No full textRegulating the Crystallization of FAPbI3-Based Perovskite with a Furan Substituted Ethylammonium Additive for Achieving Highly Efficient Solar Cells
No full textC.Z. and Q.Z. contributed equally to this work. This work was supported by the National Natural Science Foundation of China (No. U22A20142), the Hebei Province Key Research and Development Project (No. 20314305D), China Huaneng Group Co., Ltd Headquarters Science and Technology Project (HNKJ20-H88). H.Z. acknowledges the funding from the Shanghai Pujiang Program (22PJ1401200) and the National Natural Science Foundation of China (No. 52302229)
A Preclinical Model to Assess Intestinal Barrier Integrity Using Canine Enteroids and Colonoids
No full textAuthor Contributions:
Conceptualization, V.G., K.A. and J.P.M.; Data curation, V.G., A.R., C.Z., H.W., A.L., T.A., D.K.S. and D.K.M.; Formal analysis, M.P.C., V.G., V.L., D.D.-R. and C.Z.; Funding acquisition, K.A. and J.P.M.; Investigation, V.G., A.R., C.Z., H.W., T.A. and D.K.S.; Methodology, V.G., V.L., D.D.-R., A.R., C.Z., D.L., S.M., H.W., T.A. and D.K.M.; Resources, M.M.M. and K.A.; Supervision, K.A. and J.P.M.; Validation, V.G. and C.Z.; Visualization, V.G.; Writing—original draft, V.G.; Writing—review and editing, M.P.C., C.Z., K.P., D.K.M., K.A. and J.P.M. All authors have read and agreed to the published version of the manuscript.While two-dimensional (2D) cell cultures, such as Caco-2 and Madin–Darby canine kidney (MDCK) cells are widely used in a variety of biological models, these two-dimensional in vitro systems present inherent limitations in replicating the complexities of in vivo biology. Recent progress in three-dimensional organoid technology has the potential to address these limitations. In this study, the characteristics of conventional 2D cell culture systems were compared to those of canine intestinal organoids (enteroids, ENT, and colonoids, COL). Light microscopy and transmission electron microscopy were employed to evaluate the microanatomy of ENT, COL, Caco-2, and MDCK cell monolayers, while transepithelial electrical resistance (TEER) values were measured to assess monolayer integrity. The TEER values of canine ENT monolayers more closely approximated reported TEER values for human small intestines compared to Caco-2 and MDCK monolayers. Additionally, canine ENT demonstrated greater monolayer stability than Caco-2 and MDCK cells. Notably, while all systems displayed desmosomes, canine ENT and COL exclusively produced mucus. These findings highlight the potential of the canine organoid system as a more biologically relevant model for in vitro studies, addressing the limitations of conventional 2D cell culture systems.Simple Summary:
Immortalized cell lines are often used to model biological systems, such as the intestinal epithelium. Compared to immortalized cell lines, which are composed of identical cell clones, organoids derived from adult stem cells may represent a more accurate biological model, since they can differentiate into specialized intestinal epithelial cell types. In this study, we isolated adult stem cells from dog intestinal samples, which can be obtained with minimally invasive methods. These adult intestinal stem cells were grown into three-dimensional organoids, which recapitulate the superficial layers (epithelium) of the original tissue. The intestinal organoids were examined under a microscope to assess their similarity to the original tissue, then cultured and compared to two immortalized cell lines. The organoids had features similar to intestinal tissue, such as mucus production. In the 2D cell culture system, the organoids formed a more consistent layer than conventional cell lines, demonstrating similar integrity to that of the human intestine. These findings suggest that organoid cultures derived from dog intestinal adult stem cells can effectively be utilized in traditional cell culture systems.National Science Foundation (NSF)Depto. de Medicina y Cirugía AnimalFac. de VeterinariaTRUEpu
Kinematic Modeling and Deformation Mechanics in Shot Peening of Functional Ceramics
No full textAbstractThe applications of ceramics are limited by their brittle nature and low reliability. Recent studies show that compressive residual stress can be introduced in ceramics by shot peening, which can improve the contact strength and fatigue of ceramics. However, the formation mechanism of residuals stress has not been investigated yet. In this study, a pressure dependent plasticity model has been incorporated into a FE model of shot peening to shed light on the residual stress formation mechanism. Since shot velocity is the key process parameter to dominate the impact energy which determines the deformation state of surface material and the resultant residual stress, a new kinematic model of shots has also been developed by incorporating air drag and travel distance inside and outside the peening nozzle. The results have shown that the kinematic model can be used to predict shot velocity. The FE model provided an insightful understanding of the residual stress formation mechanisms
Modelling the Depth of Jet Penetration in Abrasive Waterjet Contouring of Alumina Ceramics
Full text linkPredictive mathematical models for the depth of jet penetration are presented for both straight-slit cutting and contouring by an abrasive waterjet (AWJ). The plausibility and predictive capability of the models are assessed and verified by an experimental investigation when cutting an 87% alumina ceramic. It shows that the predictions of the models are in good agreement with the experimental data
Difference-frequency generation of terahertz wave and optical gain in Sb-based quantum wells pumped by near-infrared lasers
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