67 research outputs found
Taxonomic study of the genus Gammarus (Amphipoda, Gammaridae) from Xinjiang, China, with description of a new species
No full textTong, Yan, Wang, Xiaokun, Liu, Fengsong, Li, Shuqiang, Hou, Zhonge (2022): Taxonomic study of the genus Gammarus (Amphipoda, Gammaridae) from Xinjiang, China, with description of a new species. Zootaxa 5120 (1): 97-110, DOI: https://doi.org/10.11646/zootaxa.5120.1.
Transcriptomic analysis of the housefly (Musca domestica) larva using massively parallel pyrosequencing
No full textFIGURE 3 in Taxonomic study of the genus Gammarus (Amphipoda, Gammaridae) from Xinjiang, China, with description of a new species
No full textFIGURE 3. Gammarus hoboksar Hou, sp. nov.. holotype male, from Xinjiang, China. A, head; B, upper lip; C, lower lip; D, left mandible; E, incisor of right mandible (variation with four teeth or three teeth and a small spine); F, maxilla I; G, maxilla II; H, maxilliped; I, urosomites (dorsal view).Published as part of Tong, Yan, Wang, Xiaokun, Liu, Fengsong, Li, Shuqiang & Hou, Zhonge, 2022, Taxonomic study of the genus Gammarus (Amphipoda, Gammaridae) from Xinjiang, China, with description of a new species, pp. 97-110 in Zootaxa 5120 (1) on page 102, DOI: 10.11646/zootaxa.5120.1.6, http://zenodo.org/record/638892
Gammarus Fabricius 1775
No full textKey to Gammarus species from Xinjiang, China 1. Eyes absent......................................................................... Gammarus liuruiyui - Eyes present......................................................................................... 2 2. Inner ramus of uropod III shorter than one-third of outer ramus............................... Gammarus brevipodus - Inner ramus of uropod III longer than one-third of outer ramus................................................. 3 3. merus and carpus of pereopods III–IV with long setae........................................................ 4 - merus and carpus of pereopods III–IV with short setae....................................................... 7 4. Uropod III both rami with simple setae.................................................................... 5 - Uropod III both rami with simple and plumose setae......................................................... 6 5. Calceoli of antenna II present............................................................ Gammarus simplex - Calceoli of antenna II absent............................................................ Gammarus tianshan 6. Inner ramus of uropod III shorter than four-fifth of outer ramus................................. Gammarus decorosus - Inner ramus of uropod III longer than four-fifth of outer ramus.................................. Gammarus lacustris 7. Uropod III outer ramus terminal article longer than one-fifth of first arctile............. Gammarus hoboksar Hou, sp. nov. - Uropod III outer ramus terminal article shorter than one-fifth of first article....................................... 8 8. Telson with one spine and setae on surface................................................. Gammarus tasitensis - Telson with two setae on surface......................................................... Gammarus takesensisPublished as part of Tong, Yan, Wang, Xiaokun, Liu, Fengsong, Li, Shuqiang & Hou, Zhonge, 2022, Taxonomic study of the genus Gammarus (Amphipoda, Gammaridae) from Xinjiang, China, with description of a new species, pp. 97-110 in Zootaxa 5120 (1) on pages 108-109, DOI: 10.11646/zootaxa.5120.1.6, http://zenodo.org/record/638892
Gammarus hoboksar Tong & Wang & Liu & Li & Hou 2022, sp. nov.
No full textGammarus hoboksar Hou, sp. nov. ( Figs 2–6) Material examined. Holotype: male (IZCAS-I-A1354-1), 9.0 mm, a reservoir (85.806°E, 46.799°N), altitude 1320 m, Hoboksar Mongol Autonomous County, Xinjiang, China, July 29, 2013, collected by K. Meng, Z. Yao, Z. Zhao and J. Liu. Paratypes: two males (IZCAS-I-A1354-2, 3), same date as the holotype. One male specimen was used for molecular analysis, and the sequences were submitted to GenBank (OM 720083 for 28S and OM 679216 for COI, respectively). Etymology. The species name is a noun in apposition, derived from the type locality “Hoboksar” from Mongolian language. Diagnosis. Calceoli of antenna II absent; merus and carpus of pereopods III–IV with short setae; bases of pereopods V–VII each with setae on anterior margin; epimeral plates II–III each with subacute posterodistal corner; inner ramus of uropod III about 0.79 times the length of outer ramus, terminal article of outer ramus about 0.24 times the length of first article, both rami with simple setae and plumose setae; telson cleft, with spines and setae on surface and three spines on distal end. Description of male. Based on holotype (IZCAS-I-A1354-1), 9.0 mm (Fig. 2). Head (Fig. 3A): Eyes oval. Antenna I (Fig. 6A): longer than antenna II, peduncle articles 1–3 in length ratio 1.0: 0.5: 0.3, with distal setae; flagellum with 22 articles; accessory flagellum with four articles; both primary and accessory flagella with short distal setae. Antenna II (Fig. 6B): peduncle articles 3–5 in length ratio 1.0: 3.1: 3.0; flagellum with 11 articles, last article tiny, each article with distal setae, calceoli lacking. Upper lip (Fig. 3B): ventral margin rounded, bearing minute setae. Mandible (Fig. 3D, E): asymmetrical. Left mandible incisor with five teeth; lacinia mobilis with four teeth; molar well developed and with a plumose seta; palp composed of three articles, second article with nine setae on inner margin, third article with four A-setae on outer margin, four E-setae on apical margin. Right mandible incisor with three teeth and one small spine, lacinia mobilis bifurcate. Lower lip (Fig. 3C): inner lobes indistinct, outer lobes covered with fine setae. Maxilla I (Fig. 3F): inner plate with 17 plumose setae on medial margin; outer plate with 11 serrate spines; palp biarticulate, distal article with seven stiff spines and two slender setae. Maxilla II (Fig. 3G): inner plate narrower and shorter than outer plate, with a row of plumose setae on medial margin; outer plate with long apical setae and a tiny spine. Maxilliped (Fig. 3H): inner plate with three stout apical spines and one subapical spine; outer plate with a row of blade spines and five plumose apical setae; palp with four articles, terminal article hooked. Pereon. Gnathopod I (Fig. 4A, B): coxal plate with two setae and one seta on anterior and posterior margins, respectively; basis anterior and posterior margins with long setae; merus with setae on posterodistal corner; carpus about 1.3 times as long as wide, about 0.6 times as long as propodus, anterior and posterior margins with setae; propodus oval, palm with one medial spine and 11 spines on posterior margin and surface; dactylus with one seta on outer margin. Gnathopod II (Fig. 4C, D): coxal plate with two setae and one seta on anterior and posterior margins, respectively; basis anterior and posterior margins with long setae; merus with setae on posterodistal corner; carpus about 1.5 times as long as wide, about 0.9 times as long as propodus, with clusters of setae along ventral margin; propodus subrectangular, palm with one medial spine and four spines on posterodistal corner; dactylus with one seta on outer margin. Pereopods III–VII (Fig. 5A, C, E, G, I), pereopod III (Fig. 5A, B): coxal plate with two setae and one seta on anterior and posterior margins, respectively; basis elongate, with two spines and setae on anterior margin, with clusters of setae on posterior margin; merus with one spine accompanied by one seta on anterior margin and three spines on anterodistal corner, posterior margin with groups of setae; carpus with groups of spines accompanied by setae on posterior margin and one spine on anterodistal corner; propodus with three pairs of spines accompanied by setae on posterior margin and two spines on posterodistal corner; dactylus with one plumose seta on anterior margin, and two setae at hinge of unguis. Pereopod IV (Fig. 5C, D): shorter than pereopod III; coxal plate with one seta and two setae on anterior and posterior margins, respectively; basis with clusters of setae on posterior margin; merus with one spine on anterior margin and two spines on anterodistal corner, posterior margin with groups of setae; carpus and propodus with groups of spines accompanied by setae on posterior margin; dactylus with one plumose seta on anterior margin, and two setae at hinge of unguis. Pereopod V (Fig. 5E, F): coxal plate with one seta and two setae on anterior and posterior margins, respectively; basis expanded, with setae and five spines on anterior margin, anterodistal corner with one spine and one fine seta, posterior margin with a row of 11 setae; merus with three spines accompanied by fine setae on anterior margin and one spine on posterior margin, anterodistal and posterodistal corners with spines; carpus and propodus with groups of spines on anterior margin; dactylus with one plumose seta on posterior margin, and two setae at hinge of unguis. Pereopod VI (Fig. 5G, H): coxal plate with two setae on posterior margin; basis elongate, with three long setae and four spines on anterior margin, anterodistal corner with two spines and one fine seta, posterior margin with a row of 11 setae; merus with three spines accompanied by fine setae on anterior margin and a group of spines on posterior margin, anterodistal and posterodistal corners with three and four spines accompanied by setae, respectively; carpus and propodus with groups of spines on anterior margin; dactylus with one plumose seta on posterior margin, and two setae at hinge of unguis. Pereopod VII (Fig. 5I, J): coxal plate with four setae on posterior margin; basis elongate, with three long setae and two spines on anterior margin, anterodistal corner with two spines, posterior margin with a row of 13 setae; merus with two groups of spines on anterior margin and a pair of spines on posterior margin, anterodistal and posterodistal corners with four and six spines respectively; carpus and propodus with groups of spines on anterior margin; dactylus with one plumose seta on posterior margin, and two setae at hinge of unguis. Coxal gills: coxal gills of pereopods III and IV about as long as bases; gills of gnathopod II and pereopod V longer than bases; gills of pereopods VI and VII much shorter than bases. Pleon. Epimeral plates (Fig. 6H–J): plate I ventrally rounded, with three setae on anteroventral margin and two setae on posterior margin; plate II with four spines on ventral submargin and four setae on posterior margin, posterodistal corner subacute; plate III with two setae on anteroventral margin and three spines on ventral margin, posterior margin with four setae, posterodistal corner subacute. Pleopods I–III (Fig. 6C): similar, outer ramus subequal to inner ramus, both rami fringed with plumose setae. Urosome. Urosomites (Fig. 3I): urosomites I–II with four groups of one or three spines accompanied by fine setae on dorsal margins; urosomite III with two pairs of spines accompanied by fine setae and two fine setae on dorsal margin. Uropods I–III (Fig. 6D–F): uropod I peduncle with one basofacial spine, with two spines on inner and outer margins, respectively, and with one and two spines on inner and outer distal corners; inner ramus with two marginal and four terminal spines; outer ramus with one marginal and five terminal spines. Uropod II short, peduncle with two spines on inner and outer margins, respectively, and one distal spine on each corner; inner ramus with two marginal and five terminal spines; outer ramus shorter than inner ramus, with one marginal and four terminal spines. Uropod III peduncle with two spines on surface and seven distal spines; inner ramus about 1.8 times as long as peduncle, reaching about 0.79 times the length of outer ramus, with three clusters of simple and plumose setae on inner margin, five groups of spines accompanied by plumose setae on outer margin; first article of outer ramus with plumose and simple setae on inner margin, three groups of spines accompanied by simple setae on outer margin, terminal article about 0.24 times as long as first article, with simple setae, longer than adjacent spines. Telson (Fig. 6G): cleft, 1.1 times as long as wide; left lobe with two spines accompanied by one seta on surface; right lobe with one spine accompanied by one seta and three setae on surface; each lobe with three distal spines accompanied by setae. Female unknown. Variations. Right mandible incisor with four teeth or three normal teeth and one small spine. Uropod III inner ramus with three to five clusters of simple and plumose setae on inner margin, two to five groups of spines accompanied by plumose setae on outer margin; first article of outer ramus with three or four groups of spines accompanied by simple setae on outer margin; Urosomite III with two or four groups of spines accompanied by fine setae on dorsal margin. Remarks: Gammarus hoboksar Hou, sp. nov. is closely related to G. takesensis in accessory flagellum with four articles, pereopods III and IV each with short setae on posterior margin, and epimeral plates II–III with subacute corner. The new species can be distinguished from G. takesensis by the following characters (G. takesensis in parentheses): (1) calceoli lacking (present); (2) inner ramus of uropod III about 0.79 times the length of outer ramus (0.65 times the length of outer ramus); terminal article of the outer ramus of uropod 3 about 0.24 times the length of first article (0.13 times the length of first article); and (3) both lobes of telson with spines and setae on surface (only with setae). Gammarus hoboksar Hou, sp. nov. is similar to G. tastiensis in the shape of gnathopods I–II, merus and carpus of pereopods III–IV with short setae, and uropod III both rumi with long plusome setae. It can be distinguished from G. tastiensis by the following characters (G. tastiensis in parentheses): (1) calceoli lacking (present); peduncle of antenna II with long setae (relatively short); (2) inner ramus of uropod III about 0.79 times the length of outer ramus (0.66 times); terminal article about 0.24 times the length of first article (0.16 times); and (3) right lobe of telson with one spine and setae on surface (only with setae) and three distal spines (two). Distinguishing features of all the nine species of genus Gammarus from Xinjiang can be found in the key below.Published as part of Tong, Yan, Wang, Xiaokun, Liu, Fengsong, Li, Shuqiang & Hou, Zhonge, 2022, Taxonomic study of the genus Gammarus (Amphipoda, Gammaridae) from Xinjiang, China, with description of a new species, pp. 97-110 in Zootaxa 5120 (1) on pages 100-106, DOI: 10.11646/zootaxa.5120.1.6, http://zenodo.org/record/638892
<b>Supplemental Material - Human activities significantly impact China’s net primary production variation from 2001 to 2020</b>
No full textSupplemental Material for Human activities significantly impact China’s net primary production variation from 2001 to 2020 by Yiling Cai, Xiaoping Liu, Kangyao Liu, Li Zeng, Youyue Wen, Changjiang Wu, Haoming Zhuang, and Bingjie Li in Journal of Progress in Physical Geography: Earth and Environment.</p
Disability concentration and access to rehabilitation services: a pilot spatial assessment applying geographic information system analysis
No full textPurpose: Due to geographical disparities, many people with profound or severe disabilities experience considerable delays in rehabilitation treatment, resulting in threats to quality of life. This pilot study aims to identify areas in Greater Brisbane, Australia, with a higher concentration of people with profound or severe disabilities and to evaluate access to rehabilitation services in these areas.
Methods: Data came from the 2016 Australian Census of Population and Housing and the National Health Services Directory. Four frequently used rehabilitation services by individuals with profound or severe disabilities (i.e., occupational therapy, physiotherapy, speech pathology and psychology) were the focus of the analysis. The data were analyzed using geospatial analysis methods (e.g., spatial scan statistic and network analysis).
Results: A higher concentration of rehabilitation services was found in the regions with lower disability prevalence and lower potential demand for rehabilitation services. In contrast, the regions with higher disability prevalence and higher potential demand for rehabilitation services experienced poorer access to rehabilitation services.
Conclusion: The findings are expected to inform policy decisions about the prioritization of rehabilitation resources and derive evidence for planning more responsive service delivery.
* Implications for rehabilitation
* The current study has demonstrated the utilization of geographic information system methods to facilitate rehabilitation service planning.
* Identification of disability concentration may inform locally responsive rehabilitation service delivery.
* Spatial assessment of mismatch between supply and potential demand may assist policy makers and service providers in the prioritization of rehabilitation resources.
* The current study contributes to the World Health Organization’s call for action to ensure adequate access to rehabilitation services by people with profound or severe disabilities.Griffith Health, School of Human Services and Social WorkNo Full Tex
Eukaryotic DNA Mismatch Repair In Vitro
No full textMismatch repair corrects biosynthetic errors generated during DNA replication. Mismatch repair deficiency causes a mutator phenotype and directly underlies hereditary nonpolyposis colorectal cancer and some sporadic cancers. Because of remarkably high conservation of the mismatch repair machinery between the budding yeast (Saccharomyces cerevisiae) and humans, the study of mismatch repair in yeast has provided tremendous insights into the mechanisms of this repair pathway in humans. Here we describe a set of practical protocols for how to prepare the yeast and HeLa cell-free nuclear extracts and site-specific DNA mismatch substrates, and how to carry out the in vitro mismatch repair assay. We validated the yeast cell-free system by the mismatch repair deficient strain (Δmsh2) and the complementation assay with purified yeast MutSα
Simultaneous Determination of 13 Mycotoxins in Plant Solid Beverages by UPLC-MS/MS Combined with Modified QuEChERS
Full text linkThis work aimed to develop a high-throughput analytical method based on QuEChERS coupled with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the determination of 13 mycotoxins in plant-solid beverages. The samples were homogenized and dissolved in water. QuEChERS method was used for pretreatment, ultrasonic extraction with 2% formic acid-acetonitrile solution, and purification with octadecylsilane bonded silica gel (C18). The extraction solution was then separated by a Kinetex F5 column, detected by a tandem mass spectrometry detector in multiple reaction monitoring mode (MRM), and quantified by matrix-matched standard calibration. The optimum conditions achieved limits of detections (LODs) in the range of 0.04~16.9 μg/kg, with correlation coefficient over 0.9916. The limit of quantifications (LOQs) were in the range 0.12~38.2 μg/kg. The recoveries in real plant-solid beverages ranged from 82.2% to 103.3%, with relative standard deviations (RSDs) below 9.0%. Moreover, the proposed method was applied to analyze mycotoxins in 18 plant-solid beverage samples, a positive detection rate of 4/18 was obtained, and the detected analytes included deoxynivalenol and fumonisin B3. This proposed method has the advantages of simple operation, low cost, high sensitivity and accuracy, which can be used for the high-throughput screening of multiple mycotoxins in plant-solid beverages
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