42,364 research outputs found
I'm saying au-revoir to Australia [music] /
No full textCover title.; Also available online http://nla.gov.au/nla.mus-an5471072; MUS: N, MUSM 142235 ; A, A02546.; Library's A copy measures 37 cm
The politics and economics of regulatory impact assessment
No full textThis is the author accepted manuscript. The final version is available from the publisher via the link in this record
Who is Little Enis
No full text25 x 22 cm.Folded broadside of the poem 'Who Is Little Enis?' by Johnathan Williams, with photo by Make-Hay Studios, designed by Captain Vague & the Edgarologist, signed by author 'for David', ca. 1974. 25 x 22 cm
Economical genotyping of little blue penguin (Eudyptula minor) clades from feather-based DNA
No full textDetermination of clade membership is a crucial requirement for many research questions addressing phylogeography, population structure, mating patterns, speciation, and hybridisation. The little blue penguin (Eudyptula minor) can be separated into two deeply divergent clades. However, assigning clade membership in little blue penguins requires molecular methods. Genetic sequencing can be used to identify clade membership but is expensive. Here, we present an economical alternative to the use of sequencing to determine little blue penguin clade membership. We extracted DNA from feathers using a method that produced reasonable quantities of DNA. We then amplified the D-loop section of the mitochondrial control region from total genomic DNA extracts, using the primers 'C L-tRNAglu' and 'D H-Dbox' followed by digestion with the restriction enzyme AluI. When visualised on a gel, distinctive banding patterns clearly indicated clade membership. We sequenced a subset of our samples and verified the accuracy of this method. The methods we present should facilitate little blue penguin research through a cost-effective approach to clade analysis as well as a successful technique to extract DNA from feathers when blood or tissue samples are not available
- …
