325,732 research outputs found

    Doryphoribius bindae Lisi 2011

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    81. Doryphoribius bindae Lisi, 2011 [T] DoRyphoRibius citRinus (Maucci, 1972) (Binda 1984) DoRyphoRibius bindae sp. nov. (Lisi 2011) Terra typica: Republic of South Africa (Africa) Republic of South Africa: • 34°01′S, 23°55′E; 200 m asl: Eastern Cape Province, Tzitzikama [Tsitsikamma National Park], moss. Binda (1984), Lisi (2011) Record numbers. Republic of South Africa: 1; total: 1. Remarks. This species is currently endemic to the Republic of South Africa.Published as part of Kaczmarek, Łukasz, 2017, Annotated zoogeography of non-marine Tardigrada. Part IV: Africa, pp. 1-74 in Zootaxa 4284 (1) on page 33, DOI: 10.11646/zootaxa.4284.1.1, http://zenodo.org/record/101040

    Doryphoribius amazzonicus Lisi 2011

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    125. Doryphoribius amazzonicus Lisi, 2011 [T] Doryphoribius flavus (Iharos 1966) (Pilato et al. 2001) Doryphoribius amazzonicus sp. nov. (Lisi 2011) Terra typica: Ecuador (South America) Colombia: • 11 °06′20.0′′N, 74 °03′50.0′′W; 2,200 m asl: Magdalena Department, Sierra Nevada de Santa Marta, between La Tagua and Cuchilla de San Lorenzo, sub-Andean forest, lichen on rock. Lisi et al. (2014) Ecuador: • 00° 41 ′S, 77 ° 18 ′W; 400 m asl: Type Locality: Orellana Province, Loreto, moss. Pilato et al. (2001), Lisi (2011) Record numbers: Colombia: 1, Ecuador: 1; total: 2. Remarks: Currently known only from Colombia and Ecuador, this species is very similar to D. flavus (see remarks for D. flavus).Published as part of Kaczmarek, Łukasz, Michalczyk, Łukasz & Mcinnes, Sandra J., 2015, Annotated zoogeography of non-marine Tardigrada. Part II: South America, pp. 1-107 in Zootaxa 3923 (1) on pages 53-54, DOI: 10.11646/zootaxa.3923.1.1, http://zenodo.org/record/24193

    Mixibius felix Lisi 2017, sp. nov.

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    Mixibius felix sp. nov. Fig. 1, Table 1 Type locality. Vegetation Island, Victoria Land, Antarctica: 74°47′0″S, 163°37′0″E. Material examined. (collected by Prof. Salvatore Motta between 1990.12.15 and 1991.1.12): Vegetation Island (2 specimens, holotype and one paratype, slides No. 4535, from debris of the bottom of a small lake, together with Acutuncus antarcticus (Richters, 1904) and Diphascon dastychi Pilato & Binda, 1999; and Mariella Creek (8 specimens, slides Nos. 4540–4541, from debris of the bottom, together with Acutuncus antarcticus and Diphascon dastychi). Type repository. Holotype and paratypes are preserved in the collection of Binda and Pilato in the Museum of the Department of Biological, Geological and Environmental Sciences (Section of Animal Biology “ Marcello La Greca ” of the University of Catania, Italy). Specific diagnosis. Colourless, cuticle smooth, eye spots present; bucco-pharyngeal apparatus of the Mixibius type (buccal tube rigid, without ventral lamina and with hook-shaped apophyses for the insertion of the stylet muscles); stylet supports inserted on the buccal tube at 65.7–68.0 percent of its length; pharyngeal bulb with apophyses and two rod-shaped macroplacoids; microplacoid and septulum absent; external claws of Isohypsibius type, internal claw of modified Isohypsibius type. Description of the holotype. Body 240 µm long, colourless, cuticle smooth, eye spots present in living specimens; bucco-pharyngeal apparatus (Figs. 1 A,B) of Mixibius type; the rigid and narrow buccal tube is 28.4 µm long and 2.5 µm wide (pt = 8.8); the ventral lamina is absent; both the dorsal and ventral apophyses for the insertion of the stylet muscles hook-shaped, slightly asymmetrical with respect to the frontal plane (Fig. 1 B of a paratype, arrows a—ventral and b—dorsal): the dorsal apophysis is slightly more stumpy, with a blunt and swollen caudal apex. A short median cuticular thickening is present caudal to both these apophyses (the ventral less visible) (Fig. 1 B). The buccal armature is not clearly visible but teeth appear to be absent (unlike Acutuncus antarcticus where well developed teeth are clearly visible). The stylet supports are inserted on the buccal tube at 67.8% of its length (pt = 67.8). Pharyngeal bulb with well-developed apophyses and two rod-shaped macroplacoids: microplacoid and septulum absent (Fig. 1 A). The first placoid, with a central narrowing, is 5.9 µm long (pt = 20.8), the second 5.4 µm (pt = 19.0) and the entire macroplacoid row 11.4 µm long (pt = 40.1). The external claws are of the Isohypsibius type, the internal are modified Isohypsibius type, the angle between the basal portion and the secondary branch being larger than 90° (Figs. 1 C,D). As a consequence of the orientation, it is particularly rare to have specimens with buccal tube and all claws correctly aligned for measurement; in the holotype it is possible to measure the internal claws I, that are 9.3 µm long (pt = 32.7); the external claws III, are 13.4 µm long (pt = 47.2), the main branches of which are the 72.9% of the total claw length; the internal claws of the same pair of legs that are 9.7 µm long (pt = 34.2) and the anterior claws IV, 10.1 µm long (pt = 35.6). The main branches of all claws have thin accessory points (Fig. 1 C, arrow); the basal extremity of the claws is slightly enlarged but lunules and other cuticular thickening on the legs are absent. Eggs not found. Etymology. The specific name felix (felix = happy) expresses the “happiness” of the species for having been finally identified and described. Remarks: The paratypes are similar to the holotype in both qualitative and quantitative characters (Table 1). Differential diagnosis. Before comparing Mixibius felix sp. nov. with the other species of the genus, taking into consideration that in some orientations the claws of Mixibius may appear to be of the Hypsibiu s type and could cause some difficulties in distinguishing between Mixibius and Acutuncus, we therefore considered it opportune to insert a comparative figures of Acutuncus (Fig. 2). Acutuncus antarcticus (Fig. 2) has a bucco-pharyngeal apparatus with the characteristic “sharp hooks” for the insertion of the stylet muscles (Figs. 2 A,B arrows) compared with the more stumpy and blunt hook-shaped of Mixibius felix sp. nov. (Fig. 1 B arrows a, b), and the Acutuncus claws are described as ‘external of the Isohypsibius type, internal of the Hypsibius type’ (Figs. 2 C,D), compared with Mixibius type claws (Fig. 1 C, D). The data for Mixibius pilatoi Wang, 2009 are from the original description of the species. Nine species are known belonging to the genus Mixibius; Mixibius saracenus Pilato, 1973, Mixibius fueginus Pilato & Binda, 1996, Mixibius ninguidus Biserov, 1999, Mixibius ornatus Pilato, Binda, Napolitano & Moncada, 2001, Mixibius sutirae Pilato, Binda & Lisi, 2004, Mixibius tibetanus Li & Li, 2008, Mixibius pilatoi Wang, 2009, Mixibius schnurae Pilato, Lisi & Binda, 2010 and Mixibius parvus Lisi, Sabella & Pilato, 2014. Only three have a smooth cuticle, therefore it seems necessary to compare Mixibius felix sp. nov. with these species: Mixibius saracenus, Mixibius fueginus and Mixibius pilatoi. Mixibius felix sp. nov. can be distinguished from Mixibius pilatoi by: lacking lunules; clearly longer placoids (the pt values of the first and second placoids are about 19–22 and 15–19 respectively in the new species, and about 13–14 and 9 respectively in Mixibius pilatoi) (see figures 1A, and 2B in Wang 2009); stylet supports inserted on the buccal tube in a more cephalic position (pt = 65.7–68.0 in the new species, 67.9–73.9 in Mixibius pilatoi according to Wang (2009); and higher values relative to the claw lengths (Table 1). The new species is very similar to Mixibius fueginus (Fig. 3) and Mixibius saracenus (Fig. 4). However, it differs from Mixibius fueginus by: having slightly narrower buccal tube (pt = 8.0– 10.8 in the new species, 11.1 in the unique known specimen of Mixibius fueginus, luckily of similar body length) (Table 1); the stylet supports inserted on the buccal tube in a more caudal position (pt = 65.7–68.0 in the new species, c. 62 in Mixibius fueginus) (Table 1); and in details of the claw shape (they are less slender in the new species (Figs. 1 C,D and 3B,C) and, in particular, the internal claws have a slightly shorter secondary branch, which narrows more gradually, whereas the internal claws of Mixibius fueginus narrow more abruptly and, as a consequence, the branch appear to have a larger proximal portion and a clearly thinner distal portion (Figs. 1 C,D and 3 B,C). Mixibius felix sp. nov. differs from Mixibius saracenus by: having eye spots; narrower buccal tube (pt = 8.0– 10.8 in the new species, 13.7–13.9 in Mixibius saracenus (Table 1 and Figs. 1 A,B and 4A); and in the claw shape: the basal portion is shorter and stouter, and both branches are shorter and stouter in the new species (Figs. 1 C,D and 4B,C).Published as part of Lisi, Oscar, 2017, Two new species of Eutardigrada from Victoria Land, Antarctica, pp. 541-558 in Zootaxa 4317 (3) on pages 542-547, DOI: 10.11646/zootaxa.4317.3.6, http://zenodo.org/record/88480

    Título: Odas a Lisi

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    Cada grupo de poesÞas con portadilla propiaSign.: a\p8\s, *\p7\s, A-S\p8\s, T\p6\sCada Parte con portadilla propia ; además otras portadillasAntepSign.: a¡12, *¡3, A-M¡12, N¡6Contiene: Parte Primera - Parte SegundaContiene: "Obras anacreónticas" ; "La inconstancia. Odas a Lisi" ; "La paloma de filis" ; "Letrillas" ; "Romances pastoriles

    Parascon nichollsae Pilato & Lisi, 2004, n. sp.

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    Parascon nichollsae n. sp. (Fig. 2. A –D) Description of the holotype: Body length 220.0 µm, colorless, cuticle smooth without pores, eye spots absent. Bucco­pharyngeal apparatus of Parascon type (Fig. 2 A); buccal tube rigid without ventral lamina, pharyngeal tube rigid without spiral thickening; apophyses for the insertion of the stylet muscles shaped like flat ridges; their caudal processes (very thin and pointing laterally in the genus) not visible due to the orientation of the specimen. No cuticular drop­like thickening present between buccal and pharyngeal tube. Stylet furcae very small, with very short branches, not swollen. Stylet supports present. Total length of the bucco­pharyngeal tube 20.8 µm; buccal tube 15.5 µm long (pbf = 74.5) and 1.5 µm wide (pt = 9.7). Pharyngeal bulb without apophyses and placoids (Fig. 2 A, C). Claws of Hypsibius type (Fig. 2 C); main branches with thin accessory points. On all legs the external claws have, as the other species of genera Parascon, Itaquascon and Astatumen, a slightly forked base. Due to the orientation, we were able to measure only the external claws of the first pair of legs (12.7 µm long: pt = 81.9), the external claws of the second pair of legs (13.4 µm long: pt = 86.5), and the internal claws of the second pair of legs (5.7 µm: pt = 36.8). Lunules and other cuticular thickenings on the legs absent. Differential diagnosis: Up to now only one species of genus Parascon is known: Parascon schusteri Pilato & Binda 1987 (Fig. 2 B) from Tanzania. The new species differs from it in the following features: shorter pharyngeal tube length in relation to the total length of the bucco­pharyngeal tube (see Fig. 2 and the values of the pbf index in table 1), shorter claws with lower values of the pt index (table 1). Bertolani, R. (1984) Tardigradi muscicoli delle dune costiere italiane, con descrizione di una nuova specie. Atti Società Toscana Scienze Naturali, Memorie, S. B, XC, 139–148. Binda, M. G. & Pilato, G. (1969) Tardigradi muscicoli dell’isola di Ustica (Sicilia), con descrizione di due specie nuove. Bollettino Accademia Gioenia Scienze naturali, Catania, S. IV, X, 2, 171 – 180. Binda, M. G., Pilato, G. & Dastych, H. (1980) Descrizione di una nuova specie di eutardigrado: Doryphoribius macrodon. Animalia, 7, 23– 27. Marcus, E. (1928) Bärthierchen (Tardigrada). In: Dahl, F., Die Tierwelt Detschlands und der angrenzenden Meeresteile. Jena, 12, IV, 1–230. Moon, S. Y., Kim, W. & Bertolani, R. (1994) Doryphoribius koreanus, a new species of Tardigrada from Korea. Proceedings Biological Society Washington, 170 (3), 514–516. Morgan, C. I. & Nicholls, C. A. (1986) Apodibius serventyi sp. nov., a new clawless water­bear (Invertebrata: Tardigrada) from Western Australia. Journal Royal Society Western Australia, 69, I, 1­4. Pilato, G. (1981) Analisi di nuovi caratteri nello studio degli Eutardigradi. Animalia, 8, 51– 57. Pilato, G. & Binda, M. G. (1987) Parascon schusteri n. gen. n. sp. (Eutardigrada Hypsibiidae, Itaquasconinae). Animalia, 14, 91– 97. Pilato, G., Binda, M. G. & Claxton, S. (2002) Itaquascon unguiculum and Itaquascon cambewarrense: two new species of eutardigrades from Australia. New Zealand Journal of Zoology, 29, 87– 93.Published as part of Pilato, Giovanni & Lisi, Oscar, 2004, Doryphoribius neglectus sp. n. and Parascon nichollsae sp. n., new species of eutardigrades from Australia, pp. 1-7 in Zootaxa 545 on pages 4-7, DOI: 10.5281/zenodo.15754

    Milnesium validum Lisi 2017, sp. nov.

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    Milnesium validum sp. nov. Fig. 6, Table 2 Type locality. Antarctica: Shore of Carezza Lake, 71° 43’ S 164° 03’E. Material examined. (collected by Prof. Salvatore Motta between 1990.12.15 and 1991.1.12): shore of Carezza Lake: the holotype (slide No. 4035) and two paratypes (slides nos. 4036–4037) in a moss sample; Lamplugh Island (75° 34’S 162° 55’E): two paratypes (slides Nos. 4038 and 4047) in a moss sample, together with Acutuncus antarcticus; Edmonson Point (74° 21'S, 165° 10'E): two exuviae (slide No. 4039 with 2 eggs, and 5777 with 9 eggs) in a moss sample, together with Acutuncus antarcticus. Type repository. Holotype and paratypes are preserved in the collection of Binda & Pilato in the Department of Biological, Geological and Environmental Sciences (Section of Animal Biology “ Marcello La Greca ” of the University of Catania, Italy). Specific diagnosis. Body colourless; cuticle smooth; eye spots present; six peribuccal and two lateral papillae present; mouth terminal with six triangular peribuccal lamellae with basal stripes; cylindrical buccal tube not very wide; stylet supports inserted on the buccal tube at 61.1–64.8% of its length; claws of the Milnesium type with a [3- 3]-[3-3] configuration; primary branches with thin accessory points; secondary claws stout, with distal portion clearly wider than the basal portion; each with a rounded basal thickening (lunule); a long cuticular bar present under claws I–III. Description of the holotype. Body 482 µm long; colourless; cuticle smooth without pseudopores, reticulum, tubercles or gibbosities; eyes present; six cephalic and two lateral papillae present; bucco-pharyngeal apparatus of Milnesium type (Fig. 6 A) (buccal tube rigid without ventral lamina; apophyses for the insertion of the stylet muscles in the shape of very short and flat ridges symmetrical with respect to the frontal plane and without caudal processes; pharyngeal bulb without apophyses, placoids or septulum). Mouth terminal with six triangular peribuccal lamellae with basal stripes; buccal tube cylindrical 55.6 µm long, not very wide (16.6 µm externally at the level of the stylet supports insertion point, pt index value = 29.9); stylet supports inserted on the buccal tube at 62.0% of its length (pt = 62.0); stylet furca with well-developed branches (Fig. 6 A); pharyngeal bulb pear-shaped. Claws of the Milnesium type (Figs. 6 B–D); secondary claws stout, with the distal portion clearly wider than the basal portion (Figs. 6 B–D) and with three points, configuration [3-3]-[3-3]; the second point (point b, according to the terminology used by Binda & Pilato 1990) is clearly longer and wider than the other two, particularly at the distal point (Figs. 6 B–D). Primary claws on legs I, 20.0 µm long (pt = 36.0) and secondary claw, 14.5 (pt = 26.1); the percent ratio secondary/primary claw is 72.5; primary claws on legs II, 20.7 µm long (pt = 37.2); secondary claw II, 15.0 µm long (pt = 27.0); the secondary/primary claw percent ratio is 72.5; primary claws on legs III, 22.0 µm long (pt = 39.6), secondary claw III could not be measured; primary claws on legs IV, 26.9 µm long (pt = 48.4), secondary claws on legs IV, 16.9 µm (pt = 30.4); the secondary/primary claw percent ratio is 62.8. Primary claws with thin accessory points; each secondary claw base with rounded basal thickening (lunule) (Fig. 6 D, arrow); a long cuticular bar is present under the claws I–III (Fig. 6 B, arrow). Eggs smooth laid in the exuvium (we found an exuvium with two eggs and another with nine eggs). Remarks: The paratypes are similar to the holotype in both qualitative and quantitative characters (Table 2). Together with Milnesium validum sp. nov. we found specimens of Acutuncus antarcticus. Etymology: the specific name validum (validus = vigorous) refers to the robustness of the secondary claws. Milnesium validum sp. nov Differential diagnosis: Within the genus Milnesium twelve species with smooth cuticle, six peribuccal lamellae and [3-3]-[3-3] claw configuration are known: Milnesium brachyungue Binda & Pilato, 1990; Milnesium eurystomum Maucci, 1991; Milnesium asiaticum Tumanov, 2006; Milnesium antarcticum Tumanov, 2006; Milnesium longiungue Tumanov, 2006; Milnesium zsalakoae Meyer & Hinton, 2010; Milnesium barbadosense Meyer & Hinton 2012; Milnesium bohleberi Bartels, Nelson, Kaczmarek & Michalczyk, 2014, Milnesium shilohae Meyer, 2015; Milnesium minutum Pilato & Lisi 2016; Milnesium sandrae Pilato & Lisi, 2016 and Milnesium tumanovi Pilato, Sabella & Lisi, 2016. In the following comparisons, and to save repartition, the detailed quantitative data of the single species are reported in Table 3 where several, significant differences are recognizable; in cases where there are few metric differences (i.e. Milnesium shilohae and Milnesium minutum) these are indicated in the text. szalokoae, Milnesium barbadosense, Milnesium tumanovi, Milnesium sandrae anđ Milnesium antrcticum in µm, with the pt inđex values, anđ the percent ratio between the seconđary anđ the primary claw lengths. The measurements relative to the holotype of Milnesium brachyungue are slightly đifferent from those inđicateđ in the đescription of the species because in this paper a unique criterion (đifferent from that useđ by Binđa & Pilato 1990) of measurement for all the species has been ađopteđ. The đata for the holotype of Milnesium szalakoae Meyer & Hinton 2010, Milnesium barbadosense Meyer & Hinton 2012, anđ the holotype of Milnesium antarcticum Tumanov, 2006 are from the original đescriptions of these species. Milnesium Milnesium Milnesium Milnesium Milnesium Milnesium Milnesium Milnesium antarcticum brachyungue asiaticum longiungue szalakoae barbadosense tumanovi sandrae . Sliđe 3940 5105 5103 3904 4290 5104 holotype paratype paratype holotype holotype Crimea: Yalta Hawaii Islanđ holotype holotype paratype holotype Bođy length 801 685 747 477 686.4 744 567?? Milnesium validum sp. nov. differs from Milnesium eurystomum and Milnesium bohleberi by having a narrower, cylindrical, instead of a wide, clearly funnel-shaped buccal tube. Milnesium validum sp. nov. differs from Milnesium brachyungue by having higher pt values of both the primary and secondary claw lengths, and lower percent ratio values between the secondary claw and primary claw lengths (Figs. 6 B–D and 7B, C; Tables 2 and 3); this ratio difference is particularly marked for legs IV where the ratio values are 60–63 in Milnesium validum sp. nov. and c. 81 in Milnesium brachyungue (Tables 2 and 3). The new species differs from Milnesium asiaticum in having longer buccal tube with respect to the body length (percent ratio c. 11 in the new species and 6.5–8 in Milnesium asiaticum) (Tables 2 and 3); secondary claws stouter with the distal portion clearly wider than the basal portion (Figs. 6 B–D and 8B,C); slightly lower values of the pt index relative to the main branches of claws I–III and, as a consequence, higher percent ratio between the secondary claw lengths and the primary claw lengths (Table 2 and 3). Milnesium validum sp. nov. differs from Milnesium longiungue by having primary claws with accessory points; stouter secondary claws with the distal portion clearly wider than the basal portion (Figs. 6 B–D and 9B, C); lower pt index values relative to primary claw lengths (the difference is particularly marked in claw IV where the pt value is 46.6–48.4 in the new species and 83–88 in Milnesium longiungue); and higher values of the percent ratio between the secondary claw and the primary claw lengths (Tables 2 and 3). The new species differs from Milnesium zsalakoae by having primary claws with accessory points; lower pt index values relative to the primary claws (the difference is particularly marked in claw IV where the pt value is 46.6–48.4 in the new species and 95–103 in Milnesium zsalakoae according to Meyer & Hinton 2010) (Tables 2 and 3); and higher percent ratio between the secondary claw lengths and the primary claw lengths (Table 2 and 3). Milnesium validum sp. nov. differs from Milnesium barbadosense by having eyes; buccal tube clearly longer with respect to the body length (percent ratio c. 11 in the new species, 7–9 on Milnesium barbadosense) (Figs. 6 A, and Fig. 1 A,B of Meyer & Hinton 2012); stylet supports inserted on the buccal tube in a more anterior position: pt = 61.1–64.8 in the new species, 66–84 in Milnesium barbadosense according to Meyer & Hinton, 2012 (Tables 2 and 3.) (N.B we think this very wide range for Milnesium barbadosense should be confirmed that it is not more than one species). The new species differs from Milnesium shilohae in having the stylet supports inserted on the buccal tube in a more cephalic position (pt = 61.1–64.8 in the new species, 75.5–77.5 in Milnesium shilohae according to Meyer 2015); by having the basal spurs almost as developed as, or more developed than the other points, while in Milnesium shilohae they are clearly less developed than the other points (Fig. 6 B, D and figure 2 of Meyer 2015). The new species differs from Milnesium minutum by having larger body size, narrower buccal tube (the pt index value is 29.9–34.4 in specimens of the new species more than 400 µm long, and about 38.6–42.4 in specimens of Milnesium minutum about 300 µm long); and it differs in having stouter secondary claws. Milnesium validum sp. nov. differs from Milnesium tumanovi by having buccal tube longer with respect to the body length (the percent ratio is c. 11 in the new species and c. 7 in Milnesium tumanovi); and narrow with respect to the buccal tube length (the pt index value is 29.9–34.4 in the new species and c. 55 in Milnesium tumanovi); stylet supports inserted on the buccal tube in a more posterior position (pt = 61.1–64.8 in the new species, c. 52 in Milnesium tumanovi); stouter secondary claws with basal spur as developed as, or more developed than the other points, while in Milnesium tumanovi they are clearly less developed than the other points (Figs. 6 B, D and figures 4B, C in Pilato et al. 2016); lower pt index values relative to the secondary claws IV, and lower percent ratio between the secondary claw length and the primary claw length of that pair of legs where the ratio values are 60–63 in the new species, and c. 76 in Milnesium tumanovi) (Tables 2 and 3). Milnesium validum sp. nov. differs from Milnesium sandrae by having buccal tube longer with respect to the body length (the percent ratio is c. 11 in the new species and c. 7 in Milnesium sandrae), and narrower with respect to the buccal tube length (the pt index is 29.9–34.4 in the new species, and 45–48 in Milnesium sandrae) (Figs. 6 A and 10A); secondary claws slightly stouter (Figs. 6 B–D and 10B,C); slightly lower values of the pt index relative to the secondary claws and lower values of the percent ratio between the secondary and the primary claws (that a ratio is 70.9–75.8 in the claws I–III and 60–63 in claw IV of the new species, while 78.6–85.5 and 70.4–71.4 respectively in Milnesium sandrae (Tables 2 and 3). Milnesium validum sp. nov. is similar to Milnesium antarcticum but the stylet supports are inserted on the buccal tube wall in a slightly more cephalic position (pt = 61.1–64.8 in the new species, 66.6–71.3 in Milnesium antarcticum); overall, by having clearly stouter secondary claws with shorter basal portion (Figs. 6 B–D and 11B– D), and with the distal point clearly more slender than the basal, while in Milnesium antarcticum this difference is less marked (Figs. 6 B–D; 11B–D, and figures 17–18 in Tumanov 2006); the pt index values of the claws I–III seem to be slightly higher in the new species (Tables 2 and 3). We have to report here and correct a misidentification published by us in a very recent paper (Pilato et al. 2016), regarding our new Milnesium species (Milnesium validum sp. nov.) and Milnesium antarcticum. In Pilato et al. (2016), we compared Milnesium tumanovi with a Milnesium antarcticum from our collection that we had erroneously attributed to Milnesium antarcticum, and which we are now describing as a new species (Milnesium validum sp. nov.). We had used our best specimen (slide No. 4035, which is now the holotype of Milnesium validum sp. nov.), as an example of Milnesium antarcticum, both for the images and measurements (see: figure 8 and table 2 in Pilato et al. 2016). This was done because our specimen was in much better state than the paratypes of Milnesium antarcticum that are deposited in our collection. As a consequence, photographs published in Pilato et al. (2016), as Milnesium antarcticum, are actually the holotype of the new species (Fig 6). To eliminate any doubts, Fig. 11 presented here is of a paratype of Milnesium antarcticum (see also figures 17 and 18 in Tumanov 2006), and in Table 3 we indicated quantitative characters of a Milnesium antarcticum paratype, and the holotype of this species according to Tumanov (2006).Published as part of Lisi, Oscar, 2017, Two new species of Eutardigrada from Victoria Land, Antarctica, pp. 541-558 in Zootaxa 4317 (3) on pages 548-554, DOI: 10.11646/zootaxa.4317.3.6, http://zenodo.org/record/88480

    Prediction of heparin binding of mutated short sequences of rat thyroglobulin

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    Background: Binding of thyroglobulin (Tg) to heparin is involved in Tg transcytosis via megalin. Rat Tg (rTg) binds to heparin through an exposed carboxyl terminal region (RELPSRRLKRPLPVK, Arg2489-Lys2503) rich in positively charged residues. This region is not entirely conserved in human Tg (hTg) (Arg2489-Glu2503, REPPARALKRSLWVE), resulting in lower affinity binding. Here, we developed a score to predict to what extent secondary structure modifications affect the heparin-binding ability of rTg. Methods: We designed eight synthetic peptides, including one with the Arg2489-Lys2503 sequence of rTg (rTgP), one with the corresponding sequence of hTg (hTgP), and six “mutant” peptides, each carrying a point mutation obtained by replacing one amino acid residue of rTgP with the corresponding residue of hTgP. Heparin binding was assessed in solid-phase assays. The Bmax and the constants of dissociation (Kd) were calculated. Results: Using a no-fee online service, we obtained predictions of peptide secondary structures and developed a scoring system to estimate to what extent mutations are expected to modify rTg secondary structure. The score was designated as Probability of Secondary Structure Change (PSSC) and it significantly correlated with the BMax (R = 0.942, P < 0.001) and the Kds (R = − 0.744, P < 0.01) of heparin binding of hTgP and of the “mutant” peptides. Conclusions: The PSSC score allows predicting to what extent point mutations are likely to affect the heparin-binding ability of short sequences of proteins: in this case rTg, regardless of whether mutations affect charge of the sequence. The secondary structure of Tg is likely to play a role in heparin binding

    The Chapman-Enskog procedure for an age-structured population model: initial, boundary and corner layer corrections

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    We consider a mathematical model of an age-structured population of some fisheries (for example, anchovies, sardines or soles). Two time scales are involved in the problem: the fast time scale for the migration dynamics and the slow time scale for the demographic process. At a first step, we study the so called 'aggregated' system by means of the semigroups theory. Then, we study the asymptotic behaviour of the model by using the Chapman-Enskog procedure. In particular, we study initial, boundary and corner layer effects in order to obtain the exact initial and boundary conditions the approximated solution has to satisfy. (c) 2005 Elsevier Inc. All rights reserved
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