118,374 research outputs found
Formation of Calcium Carbonate Deposition in the Cotyledons during the Germination of Justicia procumbens L. (Acanthaceae) Seeds
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Bulbophyllum albociliatum (Liu & Su) Nakejima var. weiminianum and Flickingeria shihfuana, Two New Native Orchids from Taiwan
No full textIn China there lived a little bittie man His name was Ching Long - Ching Ling - Chan
No full textvoiceCollected by Sue Holley For Mary Celestia Parler Transcribed by Sue Holley
Sung by Sue Holley Fayetteville, Arkansas July 28, 1959
Reel 289, Item 9
Ching Ling Chan
In China there lived a little bittie man His name was Ching Long - Ching Ling - Chan His feet was short and his legs was long This little man couldn't walk at all.
Ching Ling, Ching Ling, Ching Ling Chan Ride oh Dago you happy man Que lo disco barley oh Gallipin wallapin China oh.
Miss Sky High was short and fat
She had money and he knew that
So under her window he did go
Playing a little tune on his little banjo.
Ching Ling, Ching Ling, Ching Ling Chan Ride oh Dago you're happy man Que lo disco barley oh Gallipin wallipin China oh.
Miss Sky High with her notes of love Held her wash board up above She let it fall on Ching Ling Chan That was the end of the China man.
Ching Ling, Ching Ling, Ching Ling Chan Ride oh Dago you're happy man Que lo disco barley oh Gallipin wallipin China oh.Funding for digitization provided by the Arkansas Humanities Council and the Happy Hollow Foundation
Sequencing data and MLPA analysis data in support of the effectiveness and reliability of an asymmetric PCR-Based approach in preparing long MLPA probes
No full textAbstractABI PRISM 3100 Genetic Analyzer, a multi-color fluorescence-based DNA analysis system with 16 capillaries operating in parallel, was ideal tool both for DNA sequencing and DNA fragment analysis [1,2]. To demonstrate the effectiveness and reliability of an asymmetric PCR-Based approach (X.Y. Ling, G.M. Zhang, G. Pan, H. Long, Y.H. Cheng, C.Y. Xiang, L. Kang, F. Chen, Z.N. Chen, Preparing long probes by an asymmetric PCR-based approach for multiplex ligation-dependent probe amplification (MLPA), Anal. Biochem. (2015), http://dx.doi.org/10.1016/j.ab.2015.03.031, in press) in preparing the long MLPA probes that were generated with a M13-based method before [4], some prepared long MLPA probes were sequenced and then tested in MLPA analysis. Sequencing data shows that the long MLPA probes were identical to the designed ones, indicating the long probes can be easily prepared with the new method, and the MPLA analysis data shows that the results of MPLA analysis with these long probes were as same accurate and specific as with ones prepared with other methods. The sequencing data was not presented in the research article (X.Y. Ling, G.M. Zhang, G. Pan, H. Long, Y.H. Cheng, C.Y. Xiang, L. Kang, F. Chen, Z.N. Chen, Preparing long probes by an asymmetric PCR-based approach for multiplex ligation-dependent probe amplification (MLPA), Anal. Biochem. (2015), 10.1016/j.ab.2015.03.031, in press), but the MLPA analysis data was converted into figure 4 and figure 5 of the research article
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