28,340 research outputs found
The Politics of Reregulation: Democratization, Globalization, and the Taiwanese Labor Movement，Huang, Chang-Ling
No full text
Measuring and analyzing German and Spanish customer satisfaction of using the iPhone 4S Mobile Cloud service
No full textThis paper presents the customer satisfaction analysis for measuring popularity in the Mobile Cloud, which is an emerging area in the Cloud and Big Data Computing. Organizational Sustainability Modeling (OSM) is the proposed method used in this research. The twelve-month of German and Spanish consumer data are used for the analysis to investigate the return and risk status associated with the ratings of customer satisfaction in the iPhone 4S Mobile Cloud services. Results show that there is a decline in the satisfaction ratings in Germany and Spain due to economic downturn and competitions in the market, which support our hypothesis. Key outputs have been explained and they confirm that all analysis and interpretations fulfill the criteria for OSM. The use of statistical and visualization method proposed by OSM can expose unexploited data and allows the stakeholders to understand the status of return and risk of their Cloud strategies easier than the use of other data analysis
A study of the water power development in connection with the reservoirs for flood control, Yung Ting Ho, China
No full textThesis (M.S.)--Massachusetts Institute of Technology, Dept. of Civil Engineering, 1935.MICROFICHE COPY AVAILABLE IN ENGINEERING.Includes bibliographical references (leaf 68).by Chang-ling Chang.M.S
Railway infrastructure classification and instability identification-sentinel-1 and Lidar
No full textThis is the data archive for Chang, L., Sakpal, N., Oude Elberink, S. J., & Wang, H. (2020). Railway Infrastructure Classification and Instability Identification Using Sentinel-1 SAR and Laser Scanning Data.
Sensors (Switzerland), 20(24), 1-16. [7108]. https://doi.org/10.3390/s20247108 - Open Access
Satellite radar interferometry (InSAR) techniques have been successfully applied for structural health monitoring of line-infrastructure such as railway. Limited by meter-level spatial resolution of Sentinel-1 satellite radar (SAR) imagery and meter-level geolocation precision, it is still challenging to (1) categorize radar scatterers (e.g., persistent scatterers (PS)) and associate radar scatterers with actual objects along railways, and (2) identify unstable railway segments using InSAR Line of Sight (LOS) deformation time series from a single viewing geometry. In response to this, (1) we assess and improve the 3-D geolocation quality of Sentinel-1 derived PS using a 2-step method for PS 3-D geolocation improvement aided by laser scanning data; after geolocation improvement, we step-wisely classify railway infrastructure into rails, embankments and surroundings; (2) we recognize unstable rail segments by utilizing the (localized) differential settlement of rails in the normal direction (near vertical) which is yielded from the LOS deformation decomposition. We tested and evaluated the methods using 170 Sentinel-1a/b ascending data acquired between January 2017 and December 2019, over the Betuwe freight train track, in the Netherlands. The results show that 98% PS were associated with real objects with a significance level of 25%, the PS settlement measurements were generally in line with the in-situ track survey Rail Infrastructure aLignment Acquisition (RILA) measurements, and the standard deviations of the PS settlement measurements varied slightly with an average value of 6.16 m
Trat Changwat (Thailand), Chang island
No full textAmpur Laem Sing, Chantaburi [Chanthaburi], Tambon Goh Chang, Amphur Laem Ngop, Changwat Trat. Nov. 25. Laem Ling, east of north end of Goh Chang. 4 1/300 G.GrayscalePendleton nitrate negative, Box 187 of 38
Onychostoma brevibarba, a new cyprinine fish (Pisces: Teleostei) from the middle Chang Jiang basin in Hunan Province, South China
No full textSong, Xue-Ling, Cao, Liang, Zhang, E. (2018): Onychostoma brevibarba, a new cyprinine fish (Pisces: Teleostei) from the middle Chang Jiang basin in Hunan Province, South China. Zootaxa 4410 (1): 147-163, DOI: 10.11646/zootaxa.4410.1.
[[alternative]]A Study on Chang Hao
No full text[[abstract]]上篇為張昊先生年譜，包括︰先生家系、居鄉里時期（一歲∼十五歲
，1913∼1936年）、湖北時期（十六歲∼三十六歲，1937∼ 1946年）、
海外時期（三十六歲∼六十八歲，1947∼1979年）、返國定居時期（六十
八歲迄今，1979年∼）。下篇為張昊先生作品分析，先生的作曲歷程，依
地域可分為上海、外國（巴黎、義大利仙納城、德國柯隆）與台北三個時
期，截至1995年共有35首作品，而第三時期台北，為先生一生創作高峰。
筆者選其代表作品九首分析：（1）管絃樂曲：大理石花、華岡春曉、東
海漁帆（2）室內樂曲：梅山舞、茶山舞、深山古廟（3）合唱曲：西湖好
、關雎頌（4）協奏曲：小號協奏曲。最後結論將先生在國際漢學地位與
音樂界地位加以說明。
Part 1: The Chronicle History of Chang Hao Chang Hao's Family
Pedigree、The Period during Which He Stayed at Home（Age
1-15；1913-1936）、The Period during Which He Was Educated in
Hu-pei Province（Age 16-36；1937- 1947）、 The Period during
Which He Stayed Overseas（Age 36- 68； 1947-1979）、 The Period
during Which He Settles Down in His Mother Country（Age 68-now
；1979-now）﹒ Part 2：an Anlysis of Chang Hao's Compositions
He's compositional process can be divided--according to various
places--into the three stages：the first stage features the
works he produced in Shang-hai，the second one refers to the
works generated overseas（in Paris、in Italy、 in Germany），
while the third one encompasses the compositions he submitted
in Taipei﹒Up to 1995，he has produced thirty-five musical
compositions，and the third stage of his production（in Taipei）
was the time when he was most creative﹒In this study，Iam
going to analyze nine major works of his：（1）Orchestral Music
： Marble Flower﹔Early Spring in Hua-Gung﹔Fishing Boats in
Dong-Hai（2）Chamber Music：Meiflormont Dance﹔Tea-Mount Dance﹔
Ancient Temple Deep in the Mountains（3）Chorus Music：How Nice
Shi-hu is、 Hymn to Guan-ju（4）Concerto Music：Victoria
Part 1: The Chronicle History of Chang Hao Chang Hao's Family
Application of Ling Zhi-8 (rLZ-8) and polysaccharide (PS-G) from Ganoderma lucidum as immunomodulators for alleviation of airway inflammation in mite-induced murine model of asthma
No full text靈芝(Ganoderma Lucidum) 為一種在中國及其他亞洲國家中被廣為使用的古老漢方中藥。Ling Zhi-8 (LZ-8)為從靈芝中純化出的蛋白分子，研究發現其具有免疫調節功能。另外，多醣體(PS-G)為靈芝的另一生物活性物質。目前發現其免疫調節功能包括促進抗原呈現細胞功能、加強單核球細胞吞噬功能、提高體液性免疫力及細胞性免疫力。 氣喘為目前世界上最普遍發生的過敏性疾病，約有數百萬的兒童及成人面臨此種疾病。過敏性氣喘通常伴隨有呼吸到過度反應、嗜酸性白血球在肺部的浸潤、呼吸道黏液分泌過多等症狀。家塵螨Dermatophagoides pteronyssinus 第二群過敏原 (Der p2)為主要過敏原。因此綜合先前的研究，我們期望rLZ-8及PS-G免疫調節功能將可作為治療過敏性疾病的一個方法。此研究中，我們給予樹突細胞rLZ-8及PS-G刺激，發現rLZ-8及PS-G可以增加IL-12p40之產生，並且促進樹突細胞成熟表現CD86, CD83, and MHC Ⅱ分子。此外rLZ-8及PS-G刺激後的樹突細胞與T細胞共同培養，可以增加T細胞增生能力及產生IFN-γ及IL-4。在中和反應實驗中發現，給予TLR-4抗體可以明顯抑制樹突細胞分泌IL-12p40，顯示IL-12p40的訊息傳導是經由TLR-4路徑。更進一步地，我們利用家塵螨誘導小鼠氣喘模式來研究rLZ-8及PS-G的免疫調節能力。實驗一，小鼠致敏後連續鼻腔給予rLZ-8及PS-G研究其治療效果。實驗二，致敏時混合抗原與rLZ-8或PS-G同時給予小鼠研究其預防效果，小鼠犧牲後同樣分析呼吸道阻力、肺功能、第一型輔助型T細胞激素及第二型輔助型T細胞激素及rDer p2專一性抗體。我們的研究發現，rLZ-8及PS-G在體外實驗確實可以促進樹突細胞的成熟與活化，顯示這兩個分子具有免疫調節能力。動物實驗結果顯示，rLZ-8及PS-G可抑制呼吸道阻力產生及血清的IgE。PS-G可減少肺沖洗液及胰臟細胞IL-4和IL-5之產生，而rLZ-8可減少肺沖洗液IL-5產生。因此rLZ-8及PS-G可改善氣喘的徵狀，但其作用機制則需要更進一步地釐清。Ganoderma Lucidum, a China herb, has been widely used in China and other Asian countries. G. Lucidum has been reported to be effective in modulating immune functions. Ling Zhi-8 (LZ-8) is a protein derived from the fungus Ganoderma lucidum and the polysaccharide from Ganoderma Lucidum (PS-G) is a branched (1 → 6)-β-D-glucan moiety. Asthma is one of the most prevalent chronic airway inflammatory diseases in the world. The house dust mite, Dermatophagoides pteronyssinus (Der p), is the major allergen, and is the dominant species collected in Taiwan. Therefore, we like to investigate whether rLZ-8 and PS-G might exert a regulatory role in the treatment of allergic diseases. In this study, we first investigated the immunomodulatory effects of rLZ-8 and PS-G on BMDCs. The result showed that rLZ-8 and PS-G enhanced the expression of cell surface markers including CD86, CD83, and MHC Ⅱ, as well as the enhanced production of IL-12 p40 and IL-10. In addition, rLZ-8 or PS-G-treated BMDCs could promote T cell proliferation capacity, and increased T cell secretion of IFN-y. In animal Experiment Ⅰ, immunized female BALB/c mice were consistently intranasally received rLZ-8 and PS-G for treatment. In Experiment Ⅱ, mice were immunized (i.p.) injection of rDer p2 admixed with rLZ-8 or PS-G for investigating the preventive effect. Further, airway hyperresponsiveness, pulmonary function, TH1/TH2 cytokine production, and immunoglobulin levels will be followed. In conclusion, we demonstrated that rLZ-8 and PS-G effectively promoted the activation and maturation of immature BMDCs, suggesting that rLZ-8 and PS-G may possess a potential effect in regulating immune responses. In vivo result showed that both rLZ-8 and PS-G reduced airway hyperresponsiveness and serum IgE level. Moreover, PS-G could decrease the level of IL-4, IL-5 in BALF and splenocyte. rLZ-8 reduced IL-5 level in BALF. Although rLZ-8 and PS-G could modulate the response of asthma, the effect of these two molecules should be clarified in the future.封面
口試委員審定書
授權書
誌謝…………………………………………………………………..Ⅰ
中文摘要……………………………………………………………..Ⅲ
Abstract…………………………………………………………...IV
Contents…………………………………………………………………ⅥContents of Figures……………………………………………ⅧChapter Ⅰ. General Introduction……………………………………………… 1
1. Characterization of asthma…………………………………………. 2
2. The role of dendritic cells (DCs) and interleukin (IL)-12…………...8
3. Characterization of Der p2 allergen………………………………….9
4. Ganoderma (G.) lucidum………………………………….………...11
5. Aims of the study…………….…………………………….………..12
Chapter Ⅱ. Materials and Methods………..…………………………………...15
1. Reagents…..………………………….……………………...…...…16
2. Generation of recombinant Der p2 (rDer p2) protein..……..………19
3. Purification of rLZ-8 protein…..……………………………..…….23
4. Purification of fusion protein rDer p2-rLZ-8 ………………...……24
Chapter Ⅲ. Result of rDer p2 protein purification and rDer p2-rLZ8
fusion protein expression………….……………………………………………28
1. Expression of rDer p2 protein in yeast…..…………………….…29
2. Small-scale expression of rDer p2-rLZ8 fusion protein in yeast…29
Chapter Ⅳ. Investigate the effect of rLZ-8 and PS-G in vitro study…………31
1. Experimental propose …………………………………………….32
2. Materials and methods…………...………………………………..32
3. Result of in vitro study……………………………………………37
Chapter Ⅴ. Evaluate the effect of rLZ-8 and PS-G in vivo study…...........…41
1. Experimental propose…..………………………….…..………....42
2. Materials and methods…..………………………….………….....43
3. Result of in vivo Experiment Ⅰand Ⅱ.......………………….…..51
Chapter Ⅵ. Disscussion…………………………………………………...……56
Figures…………………………………………………………………..……….63
References………………………………………………………………..……...9
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