326 research outputs found
Letter from Caleb Foote to A. J. Muste, April 1, 1942
No full textLetter to A. J. Muste, likely from Caleb Foote, regarding the possibility of Japanese American families resettle in the Midwestern states. Author describes a recent meeting between Joseph R. Goodman, himself, and Milton Stover Eisenhower, Director of the War Relocation Authority, and correspondence with the president of Antioch College. Author writes "I think the three main question the government will ask in any such plan are 1) are defense industries nearby? 2) what will public reaction be? 3) what are the employment opportunities for the Japanese?" Author also describes situation with curfew in San Francisco: "Typical of what is happening: the other night a Japanese doctor came to the YMCA secretary in San Francisco about 7 o'clock. He had a patient that he need to operate on immediately, but a) he couldn't get a hospital in the city to take the patient, and b) in an hour he had to be back in his house til 6 AM because of the curfew, not matter what happened to the patient during thPersonal correspondence, organizational records, government documents, publications, and other papers created or collected by Joseph R. Goodman documenting the forced removal and incarceration of Japanese Americans during World War II, as well as organized resistance to incarceration. Included in the collection are records of the Japanese Young Men's Christian Association and the Japanese American Citizens' League in San Francisco, including papers of the Japanese YMCA's executive secretary Lincoln Kanai; Sakai family papers; Goodman's correspondence to and from Japanese American incarcerees, organizations opposing forced removal and incarceration of Japanese Americans, the War Relocation Authority, and others; publications, photographs, and ephemera from the Topaz Relocation Center, where Goodman taught high school; War Relocation Authority records and publications; and newspaper clippings, pamphlets, and reports about forced removal and incarceration created by various government, religious, and civic organizations, in California and nationwide
Letter from Caleb Foote to Cecilia Shepperd, National Training School, March 23, 1942
No full textLetter from Caleb Foote to Cecilia Shepperd: "Thank you for your letter with its suggestion for taking three Japanese young people in the National Training School. Since A. J. Muste sent out his request, the government has forbid any voluntary evacuation for any Japanese people, so the plan at the moment is in abeyance. Although we are pretty gloomy as to the prospects for any immediate resettlement, we will let you know as soon as anything develops. Thank you for your interest."Personal correspondence, organizational records, government documents, publications, and other papers created or collected by Joseph R. Goodman documenting the forced removal and incarceration of Japanese Americans during World War II, as well as organized resistance to incarceration. Included in the collection are records of the Japanese Young Men's Christian Association and the Japanese American Citizens' League in San Francisco, including papers of the Japanese YMCA's executive secretary Lincoln Kanai; Sakai family papers; Goodman's correspondence to and from Japanese American incarcerees, organizations opposing forced removal and incarceration of Japanese Americans, the War Relocation Authority, and others; publications, photographs, and ephemera from the Topaz Relocation Center, where Goodman taught high school; War Relocation Authority records and publications; and newspaper clippings, pamphlets, and reports about forced removal and incarceration created by various government, religious, and civic organizations, in California and nationwide
A letter to the Lay-Expositor, [electronic resource] : concerning his exposition of the orthodox system of civil rights and church power, &c. in which the merits of his system are examined and stated. Truth and Religion, rejected by the Alliance; the supports of a Protestant-Dissent. By the author of The comment on Mr. Warburton's alliance between church and state.
No full textThe author attributed to 'The comment on Mr. Warburton's alliance .. 'is Caleb Fleming.Price from imprint: price Six-Pence.Electronic reproduction.English Short Title Catalog,Reproduction of original from Bodleian Library (Oxford)
Honour and recognition in the German novel of banditry ca 1800
No full textThis article performs a reading informed by Honneth’s theory of recognition of the two best-known German novels of banditry of the 1790s, Johann Heinrich Zschokke’s Abaellino der große Bandit (1794) and Christian August Vulpius’ Rinaldo Rinaldini (1799) in an effort to understand how popular literature participates in and reflects upon the discourse on honour and recognition around 1800. Its status as popular genre makes the novel of banditry (Räuberroman) a potentially interesting source on shifts in the theory and practice of honour as experienced by ordinary Europeans at the turn of the 19th century. The genre was found to relate to the honour discourse not directly, but in the manner of a heterotopia, simultaneously located outside that discourse and referentially connected to it. Taken in isolation, the novel of banditry is not an informative source on the changing role of honour and new patterns of intersubjective recognition in late 18th century Europe. Seen as part of a particular constellation of textual production and reception, however, the genre sheds light on the aporias of honour experienced by those socially marginal ‘new readers’ intent on exploiting literature in the struggle for enhanced social recognition.Peer reviewe
Phanuelus Caleb, Mungkung & Mathai, 2015, gen. nov.
No full textPhanuelus gen. nov. Caleb & Mathai Type species. Phanuelus gladstone sp.nov. Etymology. The proposed name is for the late Dr. G. J. Phanuel (Professor, Dept. of Zoology, MCC) who worked extensively on the spiders of Madras in the early 1960s. His work has been of great significance for later workers. The name is masculine in gender. Diagnosis. Small spiders characterized by short, very high cephalothorax, high and reduced thoracic region; abdomen round, nearly spherical. Leg III distinctly longer. Two RTAs (compared to usually two RTAs, sometimes with one RTA in Aelurillus, and two peaks, separated by a V or U shaped slit in Phlegra). Differs from Langona Simon in lacking a bunch of stiff hairs projecting from the base near RTA. Differs from Stenaelurillus Simon due to the absence of an anterior tegular apophysis. Embolus longer and thinner than in Stenaelurillus. Epigynum highly sclerotized, convex surface, with copulatory openings postero-laterally (whereas these are antero-median in Aelurillus, or hidden in circular grooves in Phlegra). Description. Spiders with small body (3-4 mm), carapace short and very high, short longitudinal fovea in a rounded, pit-like shallow depression in the centre just behind PLE line midway (Figs. 26, 32). Carapace widest at beginning of posterior slope; posterior slope very steep. PME closer to PLE than ALE. Clypeus vertical, moderately high (Figs. 28, 36). Male chelicerae without any tooth. Leg III longer than leg IV (Figs. 27, 37). Abdomen small, rounded with pairs of white spots arranged in the median, lighter lateral regions seen on females (Figs. 26, 33). Male palp with enlarged bulbus, tegulum leathery, embolus thin, two RTA, one long and bent at tip which is inconspicuous, other conspicuous, short and thick (Figs. 38, 39). Epigynum highly sclerotized, copulatory openings laterally placed apart, with meandering copulatory ducts (Figs. 40, 43, 44). Affinities. Species show clear affinity to subfamily Aelurillinae in genital structures and general morphology.Published as part of Caleb, John T. D., Mungkung, Soriephy & Mathai, Manu Thomas, 2015, Four new species of jumping spider (Araneae: Salticidae: Aelurillinae) with the description of a new genus from South India, pp. 1-18 in Peckhamia 124 (1) on page 7, DOI: 10.5281/zenodo.509297
CALEB, ein aktivitäts-abhängiges herunterreguliertes Molekül
No full textTitle Page, Table of Contents
Acknowledgements 4
Abbreviations and Acronyms 6
Summary 8
Zusammenfassung 10
A. Introduction
1\. Molecular Analysis of Synaptogenesis 13
2\. Activity-independent Guidance for Synaptogenesis 14
3\. Neuronal Activity Role in Synaptogenesis 15
3.1 Neuromuscular junction 16
3.2 Visual pathway 18
4\. Activity-dependent Gene and Protein Regulation 19
4.1 Gene regulation 20
4.2 Protein regulation 21
B. Aim and Hypothesis 23
1\. PART-I: Is CALEB an activity-dependent regulated molecule 23
1.1 Hypothesis: 23
1.2 Objective: 23
2\. PART-II: What is the mechanism of the activity-dependent down regulation
23
2.1 Hypothesis: 23
2.2 Objective: 24
3\. PART-III: Is the ectodomain shedding of CALEB caused by a protease 24
3.1 Hypothesis: 24
3.2 Objective: 24
C. Materials and Methods 25
1\. MATERIALS 25
1.1 Chemicals, medium and enzymes 25
1.2 Consumables and special appliances 25
1.3 Softwares used 26
1.4 Antibodies 26
1.5 cDNAs 27
1.6 Competent cells (Bacteria) 27
1.7 Animals, cells (primary and cell lines), tissues 28
2\. METHODS 28
2.1 Cell culture methods 28
2.1.1 Maintenance of cell line 28
2.1.2 Primary cell culture 29
2.2 Biochemical methods 30
2.2.1 Electrophoresis and Western blot 30
2.2.2 Quantification of Western blots 32
2.2.3 Deglycosylation of tissue 32
2.2.4 Protein extraction from cells and tissue 33
2.2.5 Biotinylation, avidin precipitation and elution 33
2.2.6 Biotin Internalization Assay 35
2.2.7 Identification of a released CALEB fragment 36
2.2.8 Characterization of membrane associated protease that cleaves CALEB 36
2.2.9 In-vitro CALEB cleavage reaction 36
2.3 Molecular biological methods 37
2.3.1 Preparation of competent cells 37
2.3.2 Transformation 38
2.3.3 Plasmid DNA preparation 38
2.3.4 Restriction enzyme digestion 38
2.3.5 Transfection of cDNAs to cell lines 39
2.4 Histological methods 39
2.4.1 Tissue sectioning 39
2.4.2 Neurohistology staining 40
2.4.3 Immunohistochemical staining 40
2.5 Immunocytochemistry 41
2.5.1 Fixation and immunostaining 41
2.6 Post-synaptic density (PSD) preparation 42
2.6.1 Animals and reagents 42
2.6.2 Protocol for PSD preparation 42
2.7 Superior colliculus incubation experiments 44
D. RESULTS 46
I Neuronal activity dependent regulation of CALEB 46
1\. Activity dependent down regulation of cell surface CALEB 46
1.1 Characterization of CALEB in E11 chick retina 46
1.2 Cell surface CALEB down regulation with neuronal activity 49
1.3 Cell surface down regulation occurs with the activation of glutamate
receptors 51
1.4 Down regulation of CALEB is an action potential independent process 53
2\. Role of Calcium in cell surface down regulation of CALEB 54
2.1 Calcium influx is necessary for the down regulation of CALEB 54
2.2 Down regulation is a calmodulin and calcineurin dependent process 56
3\. Shedding is the cause of down regulation of cell surface CALEB 59
3.1 Down regulation of cell surface CALEB is an outcome of ectodomain shedding
59
3.2 Presence of the shed ectodomain fragment of CALEB protein 61
3.4 Presence of the membrane attached part of CALEB after shedding 62
3.5 Presence of lower fragment in the developmental stages of chick 64
4\. Metalloprotease inhibition, inhibits shedding 66
4.1 Shedding of the ectodomain occurs by membrane associated proteases 67
4.2 ADAM group of metalloproteases is responsible for shedding 68
4.3 ADAM 10 and ADAM 17 are responsible for causing the ectodomain cleavage of
CALEB 70
5\. Shedding of CALEB in the presence of pharmacological reagents that affect
intracellular signalling pathways 72
5.1 Involvement of Erk kinase pathway in shedding of CALEB ectodomain 73
5.2 Shedding is independent of protein synthesis 74
6\. Calcium dependent upregulation of total CALEB 75
6.1 Total CALEB protein upregulation upon neuronal activity 76
6.2 Total CALEB increased because of more translation 77
II. Characterization of CALEB in mouse neural tissue 79
1\. Expression of mouse CALEB 79
1.1. Tissue specific expression of mouse CALEB 79
1.2 Mouse CALEB enrichment in the synaptic junctions 80
2\. Developmental expression profile of mouse CALEB 81
2.1 Superior colliculus 81
2.2 Mouse Retina 83
3\. Activity-dependent regulation of mouse CALEB in superior colliculus 85
E. Discussion 86
1\. Characterization and Distribution of CALEB 86
2\. Activity dependent down Regulation of CALEB 88
3\. CALEB down regulation is a Calcium dependent process 91
4\. Down regulation as a consequence of CALEB ectodomain shedding 94
5\. Metaloproteases cause the ectodomain shedding in the chick retina cultures
98
7\. Upregulation of chick CALEB by neuronal activity 101
8\. Mouse CALEB expression and regulation by activity 102
F. Future prospects 104
G. LITERATURE 105
H. Appendix 121
I. Erklärung 122
J. Curriculum Vitae 123Synaptogenesis is a collaborative effort of activity-independent processes
specifying neuronal connections, and activity-dependent processes refining
these initial synaptic connections. CALEB, an activity-dependent modulated
protein, might be implicated in this refinement. The aim of my work was to
investigate the mechanism of CALEB (Chicken Acidic Leucine rich EGF-like
domain containing brain protein) down regulation, which was identified in an
initial screen. I showed that cell surface CALEB down regulation was enhanced
in retinal cultures incubated with KCl or agonist of glutamate receptors.
Modulation of extracellular calcium concentration and experiments using
blockers of calmodulin revealed the involvement of calcium in the mechanism of
down regulation. Since CALEB belongs to the EGF family of growth and
differentiation factors that undergo cell surface cleavage, I hypothesized a
similar phenomenon responsible for the cell surface down regulation of CALEB.
Isolation of a soluble component in supernatants of KCl treated cultures and a
remaining membrane-tethered part in KCl incubated retinal cultures confirmed
my hypothesis. I showed in membrane fraction incubation experiments that
cleavage of CALEB ectodomain was caused by the catalytic action of a membrane
protease, which could be prevented in the presence of metalloprotease
inhibitor. For a detailed analysis of CALEB shedding, I showed that blockade
of two ADAMs (A disintegrin and metalloprotease) with inhibitors as well as
increased expression of one ADAM resulted in a decrease of total CALEB in
comparison to cell lines co-expressing dominant negative form of the same ADAM
and CALEB. Further experiments showed the involvement of Erk kinase in
ectodomain shedding of CALEB. In another part of the project, I investigated
the expression and localization of CALEB in the visual system in order to
predict a plausible role of CALEB during development. Deglycosylation
experiments showed CALEB as a highly glycosylated brain specific protein.
Subcellularly, CALEB was enriched in the synaptic junction fractions pointing
to a possible role of CALEB in synapses. The developmentally regulated
expression of CALEB was observed in the visual system. My findings suggest
that neuronal activity induced by depolarization facilitates ectodomain
shedding of CALEB by the action of metalloproteases. The expression profile
and localization confirms CALEB to be a brain specific, glycosylated
transmembrane protein, which might have a role during the formation or
maintenance of synapses.Die Bildung von Synapsen wird durch das Zusammenspiel von aktivitäts-
unabhängigen Prozessen, die neuronale Verbindungen spezifizieren, und von
aktivitäts-abhängig Prozessen, welche die Feinabstimmung synaptischer
Verbindungen beeinflussen, bestimmt. Bei Letzterem könnte CALEB, ein
aktivitat-abhängig moduliertes Protein, von Bedeutung sein. Das Ziel meiner
Arbeit war es, den Mechanismus der aktivitäts-abhängigen Modulation von CALEB
(Chicken Acidic Leucine-rich EGF-like domain containing brain protein) zu
untersuchen. Ich konnte zeigen, dass in Retinakulturen nach Inkubation mit KCl
oder Glutamatrezeptoragonisten eine Herunterregulierung von CALEB an der
Zelloberfläche innerhalb von fünf Minuten verstärkt wird. Durch Änderung der
extrazellulären Kalziumkonzentration und durch die Verwendung von
Calmodulininhibitoren konnte die Beteiligung von Kalzium an diesem Prozess
demonstriert werden. Da CALEB ein Mitglied der EGF-Familie von Wachstums- und
Differenzierungsfaktoren ist, postulierte ich, dass es bei der
Herunterregulierung von CALEB zu einer Ektodomänenabspaltung kommt. Das
Auffinden einer löslichen Komponente im Überstand von KCl-behandelten
Kulturen, sowie eines membranständigen Restfragments in Retinakulturen
bestätigten meine Hypothese. Mittels Inkubation von Membranfraktionen konnte
ich zeigen, dass die Abspaltung der CALEB-Ectodomäne durch
Metalloproteaseinhibitoren verhindert wird. Zur genaueren Charakterisierung
der Abspaltung der CALEB-Ectodomäne wurden die beiden ADAM Proteasen (A
disintegrin and metalloprotease), ADAM 10 und ADAM 17 untersucht. Die
Blockierung von ADAM 10 und ADAM 17 mit pharmakologischen Inhibitoren
verhinderte die Spaltung von CALEB. Darüberhinaus führte eine erhöhte
Expression von ADAM 10 zu einer Abnahme des CALEB Gesamtproteins im Vergleich
zu dominant negatives ADAM 10. Weitere pharmakologischen Experimente ließen
auf eine Beteiligung der Erk-Kinase bei der Abspaltung der CALEB-Ectodomäne
schliessen. Eine weitere Aufgabe dieser Arbeit war es, die Expression und
Lokalisation von CALEB im visuellen System zu untersuchen.
Deglykosylierungsexperimente zeigten, dass es sich bei CALEB um ein in hohem
Maße glykosyliertes, Gehirn-spezifisches Protein handelt. Subzellulär wird
CALEB mittels "post-synaptic density preparation" in der "synaptischen
junction Fraktion" angereichert, was auf eine mögliche Rolle von CALEB in
Synapsen hindeutet. Eine entwicklungs-abhängig regulierte Expression von CALEB
wurde im visuellen System beobachtet. Meine Untersuchungen lassen vermuten,
dass neuronale Aktivität die Abspaltung der äußeren Domäne durch
Metalloproteasen fördert. Das Expressionsprofil und die Lokalisation von CALEB
bestätigen, dass es sich bei CALEB um ein gehirnspezifisches, glycosyliertes
Transmembranprotein handelt, welchem eine Rolle während der Bildung und
Erhaltung von Synapsen zukommen könnte
A fine picture of enthusiasm, [electronic resource] : chiefly drawn by Dr. John Scott, Formerly Rector of St. Giles's in the Fields. Wherein the danger of the passions leading in religion is strongly described. To which is added, and application of the subject to the modern Methodists, Exposing the Principles and Practices of all such. With a word or two concerning those who improperly are stiled Deists. Very useful for all Persons, who would offer unto God a reasonable Service. Dedicated to the Bishop of London.
No full textDedication signed: Eusebius, i.e. Caleb Fleming, the author of the 'Application', p. 15-32.Electronic reproduction.English Short Title Catalog,Reproduction of original from Harvard University Houghton Library
Fish Line Bob.
No full textPatent for a fish bob that can clamp and grip the fishing line. The fish bob can also be moved at will to any position with the users thumb or fingers
The life and works of James Miller, 1704-1744, with particular reference to the satiric content of his poetry and plays.
No full textPhDJames Miller was born the son of a Dorset rector in 1704. He
was himself ordained, but acquired no benefice until just before his
early death, probably because of a scathing portrayal of the Bishop
of London in one of his verse satires. At Oxford he wrote a vivacious
comedy of humours, set in the University. Its production in 1730
began his dramatic career, at a time when the number of London
theatres had just doubled, and new dramatic forms were being invented.
In 1731 his poem Harlequin-Horace, a witty inversion of
the Ars Poetica, attacked pantomime and opera, but also painted a
lively portrait of the entire theatrical world, in the tradition of
the Dunciad.
After collaborating in a translation of Moliere's works Miller
wrote two plays based on this author. Of all his dramatic works
these were the most successful with his contemporaries, and were
followed by a modernisation of Much Ado, and a ballad-opera adapted
from an afterpiece by Jean-Baptiste Rousseau, and rendered highly
topical. Miller made similar use of a recent French comedy showing
a Red Indian's reactions to civilisation, a satiric "fable" by Walsh
and Voltaire's Mahomet. A large quantity of original material was
incorporated into most of these, and this is generally satirical in
nature. The Indian is made to voice almost egalitarian sentiments.
An afterpiece, "The Camp Visitants", satirised military inaction
in the war, and was apparently banned. The manuscripts of the six
plays produced after the Licensing Act bear the examiner's deletions,
and illustrate the nature of the censorship at this time.
Miller's greatest strength is probably his flexible, vigorously
colloquial dialogue. His political satire is mostly contained in
the poetry, which attacks Walpole's administration with increasing
vehemence through the seventeen-thirties, until its fall. In 1740
two poems that used Pope in symbolic contrast to Walpole caused a
sensation. In both poetry and plays Miller is also a social satirist,
who lays unusually strong emphasis on false taste and the deterioration
of culture
The times they are a changing - post qualifying training needs of social work managers
No full textThe article will focus on the changing role of social work managers in the light of recent legislation and policy; the integration agenda; the changing relationship between statutory and voluntary agencies; and the rising expectations of service users and carers. It will link these changes to the ongoing political and societal context in which social work is practised. The importance of effective social work management will be emphasised in the context of the recent enquiries into the deaths and long-term abuse of service users. It will consider the issues for training and development of social work managers and will conclude by considering the issues for providers of training and development, with particular reference to post qualifying academic social work management courses. It will highlight the types of learning experiences social work managers require, outline consultations with students and agencies, and suggest issues for the future
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