6,286 research outputs found
Maternal high fat diet during pregnancy and lactation alters hepatic expression of insulin like growth factor-2 and key microRNAs in the adult offspring
Full text linkBackground :miRNAs play important roles in the regulation of gene functions. Maternal dietary modifications during pregnancy and gestation have long-term effects on the offspring, but it is not known whether a maternal high fat (HF) diet during pregnancy and lactation alters expression of key miRNAs in the offspring.Results: We studied the effects of maternal HF diet on the adult offspring by feeding mice with either a HF or a chow diet prior to conception, during pregnancy and lactation, and all offspring were weaned onto the same chow diet until adulthood. Maternal HF fed offspring had markedly increased hepatic mRNA levels of peroxisome proliferator activated receptor-alpha (ppar-alpha) and carnitine palmitoyl transferase-1a (cpt-1a) as well as insulin like growth factor-2 (Igf2). A HF diet induced up-regulation of ppar-alpha and cpt-1a expression in the wild type but not in Igf2 knock out mice. Furthermore, hepatic expression of let-7c was also reduced in maternal HF fed offspring. Among 579 miRNAs measured with microarray, ~23 miRNA levels were reduced by ~1.5-4.9-fold. Reduced expression of miR-709 (a highly expressed miRNA), miR-122, miR-192, miR-194, miR-26a, let-7a, let7b and let-7c, miR-494 and miR-483* (reduced by ~4.9 fold) was validated by qPCR. We found that methyl-CpG binding protein 2 was the common predicted target for miR-709, miR-let7s, miR-122, miR-194 and miR-26a using our own purpose-built computer program.Conclusion: Maternal HF feeding during pregnancy and lactation induced co-ordinated and long-lasting changes in expression of Igf2, fat metabolic genes and several important miRNAs in the offspring
Vascular endothelial growth factor restores delayed tumor progression in tumors depleted of macrophages
Full text linkGenetic depletion of macrophages in Polyoma Middle T oncoprotein (PyMT)-induced mammary tumors in mice delayed the angiogenic switch and the progression to malignancy. To determine whether vascular endothelial growth factor A (VEGF-A) produced by tumor-associated macrophages regulated the onset of the angiogenic switch, a genetic approach was used to restore expression of VEGF-A into tumors at the benign stages. This stimulated formation of a high-density vessel network and in macrophage-depleted mice, was followed by accelerated tumor progression. The expression of VEGF-A led to a massive infiltration into the tumor of leukocytes that were mostly macrophages. This study suggests that macrophage-produced VEGF regulates malignant progression through stimulating tumor angiogenesis, leukocytic infiltration and tumor cell invasion
A cooperative effect of bifunctionalized nanoparticles on recognition: Sensing alkali ions by crown and carboxylate moieties in aqueous media
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The Relations Between the Sit-to- Stand Functional Muscle Strength and Walking Capacity in Children with Mild Spastic Diplegia
No full textKinetics of catalytic isomerization of quadricyclane to norbornadiene using near infrared absorption spectroscopy: Conversion rate and diffusion motion in heterogeneous reaction
No full textFinancial analysis, asset allocation, and portfolio construction: Theory & practice
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reproduced or transmitted in any forms or by any means now known or later devised, or sorted
in a data base or retrieval system, without the prior written permission of HF Consulting. This material has been shared with permission from the author and HF Consulting
On financial statements modelling and fundamental analysis
No full textThis material is copyrighted and the author retains all rights. No part of this material may be
reproduced or transmitted in any forms or by any means now known or later devised, or sorted
in a data base or retrieval system, without the prior written permission of HF Consulting. This material has been shared with permission from the author and HF Consulting
The mathematics of statistical modelling: Abstract to specific
No full textThis material is copyrighted and the author retains all rights. No part of this material may be
reproduced or transmitted in any forms or by any means now known or later devised, or sorted
in a data base or retrieval system, without the prior written permission of HF Consulting. This material has been shared with permission from the author and HF Consulting
The optical properties of hydrophilic Hf-doped HfO2 nanoceramic films
No full text[[abstract]]The Hf-doped HfO2 (HfO2:Hf) nanoceramic films were deposited by simultaneous rf magnetron sputtering of HfO2 and dc magnetron sputtering of Hf. By doping with Hf, the lowest oxidation state of Hf (Hf0+) presented preferentially and changed the hydrophobicity of HfO2 film into hydrophilicity. On comparing with the HfO2 film, the optical transmission of HfO2:Hf film was higher and strongly increased with pressure, especially in the ultraviolet region and near infrared region. At higher pressure, HfO2:Hf film exhibited better hydrophilicity, higher linear refractive index and lower stress-optical coefficient (C) 2013 Elsevier Ltd and Techna Group S.r.l. All rights reserved.[[note]]SC
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