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Enrichment and functional characterization of Sca-1+WGA+, Lin-WGA+, Lin- Sca-1+, and Lin-Sca-1+WGA+ bone marrow cells from mice with an Ly-6a haplotype
No full textAbstract Approximately 4% to 5% of all bone marrow (BM) cells and 8% to 9% of low density BM cells from FVB/N and BALB/c mice (Ly-6a haplotype) show high to intermediate expression of Ly-6E.1 antigen, recognized by the Sca-1 antibody. Functional properties of enriched cells expressing Ly- 6E.1-allelic form of Sca-1 antigen were analyzed and correlated with the properties of cells expressing the carbohydrate binding sites for the lectin wheat-germ agglutinin (WGA). Using equilibrium density centrifugation and fluorescence-activated cell sorting, Sca-1+WGA+, Lin- WGA+, Lin-Sca-1+, and Lin-Sca-1+WGA+ cells were isolated and their splenic colony-forming unit (CFU-S) cell content, radioprotection ability, and long-term reconstitution capacity determined. Enriched Sca- 1+WGA+, Lin-WGA+, Lin-Sca-1+ and Lin-Sca-1+WGA+ cells gave rise to 1 CFU-S12 cell out of 26, 20, 21, and 15 sorted cells, respectively. When transplanted into lethally irradiated recipients (100 to 500 cells/mouse) all populations rescued 70% to 100% of recipients in a 30- day radioprotection assay and mediated survival of 40% to 80% of recipients 6 months after transplantation. Using transgenic mice as cell donors we have shown that 12 to 16 weeks after transplantation of 100 Sca-1+WGA+, Lin-WGA+, Lin-Sca-1+, and Lin-Sca-1+WGA+ cells, 40% to 80% of recipients had donor cells in BM, spleen, thymus, and lymph nodes. These results indicate that the population of cells expressing Ly-6E.1 form of Sca-1 antigen in two analyzed mouse strains with Ly-6a haplotype contains CFU-S and long-term repopulating cells. Furthermore, the data suggest that, at least in FVB/N mice, day-12 CFU-S cells and cells with long-term repopulating capacity simultaneously express Ly- 6E.1 form of Sca-1 antigen and WGA-binding molecules
Thermally stimulated depolarization and polarization current study of C-60-PU based conducting interpenetrated polymer networks (IPNs)
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Synthesis of novel conducting elastomers as polyaniline-interpenetrated networks of fullerenol-polyurethanes
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C-reactive protein in childhood non-typhi salmonella gastroenteritis with and without bacteremia
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Comparison of enterovirus 71 and coxsackievius A16 clinical illness during the Taiwan enterovirus epidemic, 1998
No full textConductive behavior of interpenetrated polyanilines in fullerenol-poly(urethane-ether) network
No full textLieb-Ly
No full textLIEB-LY
Catalogus nominalis (-)
Lieb-Ly (Littera. L., Vol. II.) ( - )
Cover ( - )
Titelseite ( - )
Li ( - )
Lie ( - )
Lif (49)
Lig (50)
Lij (68)
Lil (69)
Lim (88)
Lin (106)
Lio (236)
Lip (237)
Lis (292)
Lit (308)
Liv/Liu (320)
Lix (334)
Liz (335)
Ll (335)
Lo (339)
Loa (339)
Lob (339)
Loc (354)
Lod (392)
Loe (403)
Lof (469)
Log (470)
Loh (473)
Loi/Loj (482)
Lol (490)
Lom (495)
Lon (518)
Loo (571)
Lop (577)
Loq (577)
Lor (577)
Los (612)
Lot (633)
Lou/Lov (656)
Low (663)
Loy (669)
Lu (675)
Lua (675)
Lub (675)
Luc (696)
Lud (785)
Lue (888)
Lug (907)
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Lum (916)
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Lup (949)
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Lyb (1045)
Lyc (1046)
Lyd (1057)
Lye (1068)
Lyg (1070)
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Lyr (1117)
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Lyt (1174
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