17,856 research outputs found
TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
Full text linkThe interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification
Changes in nitrogen-containing compounds in suspension-cultured rice cells treated with NaCl
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The bovine immune milk from hyperimmunized cows inhibits airway hyperresponsiveness and airway allergic inflammation of OVA-sensitized BALB/c mice
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Ganoderma products exert immunomodulatory activities in an ovalbumin immunized murine model
No full textEffects of weight-bearing versus nonweight-bearing exercise on function, walking speed, and position sense in participants with knee osteoarthritis: a randomized controlled trial
No full textSynthesis, Curing, and Decomposition of Allylamine-Adducted 3,3’-Bismaleimidodiphenylsuhphone Resins
No full textBurial of terrigenous and marine organic carbon in the Yangtze river delta and adjacent shelf sediments
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