4,756 research outputs found
Multiple functions of LIM domain-binding CLIM/NLI/Ldb cofactors during zebrafish development
No full textThe crucial involvement of CLIM/NLI/Ldb cofactors for the exertion of the biological activity of LIM homeodomain transcription factors (LIM-HD) has been demonstrated. In this paper we show that CLIM cofactors are widely expressed during zebrafish development with high protein levels in specific neuronal cell types where LIM-HD proteins of the Isl class are synthesized. The overexpression of a dominant-negative CLIM molecule (DN-CLIM) that contains the LIM interaction domain (LID) during early developmental stages of zebrafish embryos results in an impairment of eye and midbrain-hindbrain boundary (MHB) development and disturbances in the formation of the anterior midline. On a cellular level we show that the outgrowth of peripheral but not central axons from Rohon Beard (RB) and trigeminal sensory neurons is inhibited by DN-CLIM overexpression. We demonstrate a further critical role of CLIM cofactors for axonal outgrowth of motor neurons. Additionally, DN-CLIM overexpression causes an increase of Isl-protein expression levels in specific neuronal cell types, likely due to a protection of the DN-CLIM/LIM-HD complex from proteasomal degradation. Our results demonstrate multiple roles of the CLIM cofactor family for the development of entire organs, axonal outgrowth of specific neurons and protein expression levels
Highly efficient, high power, inband-pumped Erbium/Ytterbium-codoped fiber laser
No full textA highly efficient (~80%), high power (18.45W) in-band pumped Erbium/Ytterbium-codoped fiber laser is demonstrated. Based on a fitted simulation model, the detrimental effect of pair induced quenching in high power operation is also highlighted
High-energy, in-band, cladding-pumped erbium doped pulsed fiber lasers
No full textWe demonstrate a high-energy in-band, cladding-pumped MOPA at 1563nm that is >1.5 times more efficient than the equivalent core pumped MOPA variant. A maximum pulse energy of 1.4mJ was obtained for 159ns pulses with M2=1.5
High energy in-band pumped erbium doped pulse fibre laser
No full textWe demonstrate an inband, core-pumped master oscillator power amplifier (MOPA) with a maximum pulse energy of 1.56 mJ at a repetition rate of 1.25 kHz, seeded by an actively Q-switched Erbium/Ytterbium-codoped fiber (EYDF) ring laser, producing 150 ns pulses at 1562.5 nm. To maximize energy extraction whilst minimizing signal saturation effects, a 40 µm Er3+-doped larger mode area (LMA) fiber was used as the gain medium. A 1535 nm single mode fiber laser was used for in-band pumping of the LMA fiber. The output beam quality (M2) was measured to be ~1.6. This is to the best of our knowledge is the highest reported pulse energy for a pulse fiber laser at 1.5 µm with M2~1.6
High-resolution broadly-tunable MOPA-based terahertz spectrometer to non-destructively probe and modulate protein electrodynamics
No full textWe report on the development of a high-resolution (< 1 GHz at 1.0 THz), broadly-tunable (~0.5-3.0 THz) fiber MOPA-based terahertz spectrometer system. The flexibility and performance of this system will be demonstrated with gaseous (water vapor) and aqueous (water and solvated protein) samples with a view towards selectively and non-destructively probing functionally significant large-scale protein motions
Rapporteur’s report – innovative geotechnologies for energy transition
No full textThe 9th Society for Underwater Technology (SUT) International Conference on Offshore Site Investigation and Geotechnics (OSIG) closed with a Rapporteur’s report given by the author. This paper provides a record of that report, transcribed from a video recording. The presentation slides are shown as Figures.</p
DEFRApH - Sample collection and handling procedures
No full textAll chemical and biogeochemical process in the sea are affected by the acidity of the water. Acidity is therefore fundamental property of seawater. The growing concern that the acidity of the oceans might be increasing has revealed weaknesses in our knowledge of this fundamental property and its variation in space and time. In 2008 the DEFRApH project (DEFRA contract ME4133) was initiated to provide this missing information in UK related waters. It required sampling for and analysis of the total inorganic carbon and total alkalinity content of samples. This reports documents the procedures sued for sampling. A companion document Hartman Dumousseaud and Roberts (NOC Internal Document No. 01) describes in detail the analytical procedures used and the calculation of the results
Author Correction:Prefrontal cortical ChAT-VIP interneurons provide local excitation by cholinergic synaptic transmission and control attention (Nature Communications, (2019), 10, 1, (5280), 10.1038/s41467-019-13244-9)
No full textThe original version of this Article contained an error in the spelling of the author Wilma D.J. van de Berg, which was incorrectly given as Wilma D.J. van den Berg. This has now been corrected in both the PDF and HTML versions of the Article.</p
Multi-watt all-fiber frequency doubled laser
No full textWe report a record multi-watt level and up to 45% efficient all-fiber frequency doubling in periodically poled silica fibers. Modulation instability is identified as the primary factor preventing further growth of the second-harmonic power
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