15 research outputs found
Literacy support for Indigenous people: current systems and practices in Queensland
No full textNarelle McGlusky and Lenora Thaker explore the literacy and numeracy support systems available to Indigenous VET students and determine which systems work for both students and teachers and produce positive outcomes for Indigenous students, including successful completion. They outline a set of best practice guidelines for designing and developing literacy and numeracy support systems for Indigenous students
A retrospective and prospective chart review with implementations: preventing non-urgent emergency department visits from a primary care practice
No full textPurpose: Often there are patients who visit the emergency department for non-urgent complaints that can be treated in the primary care setting. This can lead to overcrowding of the emergency department, increased costs, and delay in treatment times. Providers need to advise or educate patients on the use of the emergency department versus the primary care practice. Doing so may lead to better use of the primary care providers, decrease non-urgent visits to the emergency departments and improve patient outcomes.
Methodology: A retrospective chart review with implementation of a handout was on display at a privately owned primary care practice in northern New Jersey. The handout displayed examples of services available in the emergency department versus those in the primary care practice. Prospective evaluation via a chart review of patient utilization of the emergency department was collected after handout has been available to patients. Data was analyzed for a decrease in patients utilizing the emergency department. The retrospective chart review, implementation of patient viewing handouts, and prospective chart review was utilized to develop recommendations based on the findings to promote primary care use and help decrease non-urgent emergency department visits.
Results: The statistical results revealed that most of the emergency department visits were emergent. Although emergency department visits after the implementation, the results were not statistically significant due to small sample size.
Implications for Practice: One time evaluation of patient Rapid Estimate of Adult Literacy in Medicine (REALM) score to determine baseline healthcare literacy. This will assist in educating of the patient and providers to determine what level of counseling they can provide the patient. Recommendations for additional practice hours as well as having additional providers to assist with patient volume. Utilization of call service when office is closed. Documentation of counseling of the patient for reimbursement.D.N.P.Includes bibliographical reference
Author response
No full textA well-characterized metabolic landmark for aggressive cancers is the reprogramming from oxidative phosphorylation to aerobic glycolysis, referred to as the Warburg effect. Models mimicking this process are often incomplete due to genetic complexities of tumors and cell lines containing unmapped collaborating mutations. In order to establish a system where individual components of oncogenic signals and metabolic pathways can be readily elucidated, we induced a glycolytic tumor in the Drosophila wing imaginal disc by activating the oncogene PDGF/VEGF-receptor (Pvr). This causes activation of multiple oncogenic pathways including Ras, PI3K/Akt, Raf/ERK, Src and JNK. Together this network of genes stabilizes Hifα (Sima) that in turn, transcriptionally up-regulates many genes encoding glycolytic enzymes. Collectively, this network of genes also causes inhibition of pyruvate dehydrogenase (PDH) activity resulting in diminished ox-phos levels. The high ROS produced during this process functions as a feedback signal to consolidate this metabolic reprogramming
Comparative studies on structural properties and antimicrobial potential of spinel ferrite nanoparticles synthesized using various methods
No full textLizards from suburban areas learn faster to stay safe
No full textEnhanced cognitive ability is beneficial in unpredictable and harsh environments, as it enables animals to respond with flexibility. For animals living in urbanized areas, local environments not only are altered but can rapidly change during their lifetime. Urban residents are therefore challenged with identifying novel dangers and safe refuges in dynamic environments. We demonstrate that the tropical agamid lizard Psammophilus dorsalis experiences dramatically different habitats not only across the rural to urban spatial scale but also over the short temporal scale of a few years in suburban areas. Differences in environmental stability are expected to affect rates of learning and reversal learning in resident lizards. In testing arenas, lizards from these populations were required to choose a designated 'safe' refuge instead of an 'unsafe' one after simulated predator attacks. The contingency for safety was switched during the reversal learning task. In general, P. dorsalis showed high rates of learning and reversal learning, but lizards from suburban areas were quicker to learn and unlearn the location of the safe refuge than those from rural areas. This demonstrates for the first time to our knowledge that suburban lizards have faster learning and reversal learning skills for a key survival-related behaviour, finding safety in unpredictable environments. © 2019 The Author(s) Published by the Royal Society. All rights reserved
Absence of xenotropic murine leukaemia virus-related virus in UK patients with chronic fatigue syndrome
No full textBackground: Detection of a retrovirus, xenotropic murine leukaemia virus-related virus (XMRV), has recently been reported in 67% of patients with chronic fatigue syndrome. We have studied a total of 170 samples from chronic fatigue syndrome patients from two UK cohorts and 395 controls for evidence of XMRV infection by looking either for the presence of viral nucleic acids using quantitative PCR (limit of detection <16 viral copies) or for the presence of serological responses using a virus neutralisation assay.
Results: We have not identified XMRV DNA in any samples by PCR (0/299). Some serum samples showed XMRV neutralising activity (26/565) but only one of these positive sera came from a CFS patient. Most of the positive sera were also able to neutralise MLV particles pseudotyped with envelope proteins from other viruses, including vesicular stomatitis virus, indicating significant cross-reactivity in serological responses. Four positive samples were specific for XMRV.
Conclusions: No association between XMRV infection and CFS was observed in the samples tested, either by PCR or serological methodologies. The non-specific neutralisation observed in multiple serum samples suggests that it is unlikely that these responses were elicited by XMRV and highlights the danger of over-estimating XMRV frequency based on serological assays. In spite of this, we believe that the detection of neutralising activity that did not inhibit VSV-G pseudotyped MLV in at least four human serum samples indicates that XMRV infection may occur in the general population, although with currently uncertain outcomes
DNA barcoding and traditional taxonomy: An integrated approach for biodiversity conservation
No full textBiological diversity is depleting at an alarming rate. Additionally, a vast amount of biodiversity still remains undiscovered. Taxonomy has been serving the purpose of describing, naming, and classifying species for more than 250 years. DNA taxonomy and barcoding have accelerated the rate of this process, thereby providing a tool for conservation practice. DNA barcoding and traditional taxonomy have their own inherent merits and demerits. The synergistic use of both methods, in the form of integrative taxonomy, has the potential to contribute to biodiversity conservation in a pragmatic timeframe and overcome their individual drawbacks. In this review, we discuss the basics of both these methods of biological identification- traditional taxonomy and DNA barcoding, the technical advances in integrative taxonomy, and future trends. We also present a comprehensive compilation of published examples of integrative taxonomy that refer to nine topics within biodiversity conservation. Morphological and molecular species limits were observed to be congruent in ~41% of the 58 source studies. The majority of the studies highlighted the description of cryptic diversity through the use of molecular data, whereas research areas like endemism, biological invasion, and threatened species were less discussed in the literature.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
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The structural basis for regulation of the glutathione transporter Ycf1 by regulatory domain phosphorylation
No full textYeast Cadmium Factor 1 (Ycf1) sequesters heavy metals and glutathione into the vacuole to counter cell stress. Ycf1 belongs to the ATP binding cassette C-subfamily (ABCC) of transporters, many of which are regulated by phosphorylation on intrinsically-disordered domains. The regulatory mechanism of phosphorylation is still poorly understood. Here, we report two cryo-EM structures of Ycf1 at 3.4 Å and 4.0 Å resolution in inward-facing open conformations that capture previously unobserved ordered states of the intrinsically disordered regulatory domain (R-domain). R-domain phosphorylation is clearly evident and induces a topology promoting electrostatic and hydrophobic interactions with Nucleotide Binding Domain 1 (NBD1) and the Lasso motif. These interactions stay constant between the structures and are related by rigid body movements of the NBD1/R-domain complex. Biochemical data further show R-domain phosphorylation reorganizes the Ycf1 architecture and is required for maximal ATPase activity. Together, we provide insights into how R-domains control ABCC transporter activity. © 2022, The Author(s).Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]
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Endocrine and behavioural features of Lowe syndrome and their potential molecular mechanisms.
No full textLowe syndrome (LS) is an X linked disease caused by pathogenic variants in the OCRL gene that impacts approximately 1 in 500 000 children. Classic features include congenital cataract, cognitive/behavioural impairment and renal tubulopathy.
This study is a retrospective review of clinical features reported by family based survey conducted by Lowe Syndrome Association. Frequency of non-ocular clinical feature(s) of LS and their age of onset was summarised. An LS-specific therapy effectiveness scale was used to assess the response to the administered treatment. Expression of OCRL and relevant neuropeptides was measured in postmortem human brain by qPCR. Gene expression in the mouse brain was determined by reanalysis of publicly available bulk and single cell RNA sequencing.
A total of 137 individuals (1 female, 89.1% white, median age 14 years (range 0.8-56)) were included in the study. Short stature (height OCRL is expressed in human and mouse hypothalami, and in hypothalamic cell clusters expressing Ghrh, Sst, Oxt, Pomc and pituitary cells expressing Gh and Prl.
There is a wide spectrum of the clinical phenotype of LS. Some of the features may be partly driven by the loss of function of OCRL in the hypothalamus and the pituitary.
© Author(s) (or their employer(s)) 2022. No commercial re-use. See rights and permissions. Published by BMJ.</CopyrightInformation
