7,285 research outputs found
Regression trees for regulatory element identification
No full textMotivation: The transcription of a gene is largely determined by short sequence motifs that serve as binding sites for transcription factors. Recent findings suggest direct relationships between the motifs and gene expression levels. In this work, we present a method for identifying regulatory motifs. Our method makes use of tree-based techniques for recovering the relationships between motifs and gene expression levels. Results: We treat regulatory motifs and gene expression levels as predictor variables and responses, respectively, and use a regression tree model to identify the structural relationships between them. The regression tree methodology is extended to handle responses from multiple experiments by modifying the split function. The significance of regulatory elements is determined by analyzing tree structures and using a variable importance measure. When applied to two data sets of the yeast Saccharomyces cerevisiae, the method successfully identifies most of the regulatory motifs that are known to control gene transcription under the given experimental conditions, and suggests several new putative motifs. Analysis of the tree structures also reconfirms several pairs of motifs that are known to regulate gene transcription in combination.T.M.P. was also supported by the Korea Foundation for Advanced Studies.
This work was supported by the Korean Systems Biology Research Grant (M10309020000-03B5002-00000) from the Ministry of Science and Technology
Marriage record of Harmeling, Charles and Weir, Bertha Lee
No full textMarriage license for Charles Harmeling and Bertha Lee Weir. T.M. Wier was the Notary Public
Land use changes imperil South-East Asian biodiversity
No full textNavjot S. Sodhi, Mary Rose C. Posa, Kelvin S.-H. Peh, Lian Pin Koh, Malcolm C.K. Soh, Tien Ming Lee, Janice S.H. Lee, Thomas C. Wanger and Barry W. Broo
Chemical amendments effect on the cadmium bioavailability of contaminated soils in Taiwan
No full textRetrieval and reference
No full textKnowledge from study may be transferred in different ways: in words and images, via lectures and exhibitions, in the form of articles or books; and electronically.For the time being, the form used most frequently is written publication in text and illustration. However publishing on CD-ROM and the Internet are witnessing rapid development. Maybe this is going to have important consequences for the way in which people are searching for information. In this contribution we discuss some points needing attention for optimal accessibility of knowledge from study and suitably dealing with the sources used. We refer to handbooks for the conventional playing rules of reporting in writing such as clear and interest evoking titles of chapters and paragraphs, clear structure and table of contents, avoiding unnecessary jargon, a clear summary and their like.a,b The emphasis in this Chapter is on adequate pointers to references and the use of key-words.Before embarking, first, something about the way to stimulate potential readers to take notice of the information. It starts already with the cover and the titlepage. These give a first impression of what is waiting for the potential reader. With this author, text or images present themselves. One glance should make clear what the subject is; although it is sometimes attractive to confuse the reader. Starting from cover and title page, the reference data (copyright notice, year of publication, ISBN number, place of issue and publisher), table of contents, foreword (written by a recommending outsider or referee) and introduction, the reader is introduced from his own world into the world of the author. The author and those responsible for the lay-out should picture themselves in this process and shape the publication from the vantage point of potential readers (the target audience), their questions, their pre-suppositions, or lack thereof.Possible pre-suppositions of the reader should be supplemented or corrected. With this it is prevented that potential readers are thinking after a while “What the hell is this?” A clear text on the back cover, an index of key-words, a list of references and a sensible use of footnotes and final-notes are important conditions as well in order to achieve a publication that invites reading.Technical Ecology and Methodology (OLD)Real Estate Managemen
Sounds From Fort Delaware.
No full textsectionalpianoads on back cover for Lee & Walker stock8823.4Johns Hopkins University, Levy Sheet Music Collection, Box
092, Item 113Composed for the Piano by T.M. Todd.T. Sinclair's Lith. Phila
Sounds From Fort Delaware.
No full textsectionalpianoads on back cover for Lee & Walker stock8823.4Johns Hopkins University, Levy Sheet Music Collection, Box
092, Item 113Composed for the Piano by T.M. Todd.T. Sinclair's Lith. Phila
Stiffness-force feedback in UAV tele-operation
No full textAerospace Design, Integration and OperationsAerospace Engineerin
Bacterial nitric oxide detoxification prevents host cell S-nitrosothiol formation: a novel mechanism of bacterial pathogenesis
No full textS-nitrosylation is an important mediator of multiple nitric oxide-dependent biological processes, including eukaryotic cellular events such as macrophage apoptosis and proinflammatory signaling. Many pathogenic bacteria possess NO detoxification mechanisms, such as the nitric oxide reductase (NorB) of Neisseria meningitidis and the flavohemoglobins (Hmp) of Salmonella enterica and Escherichia coli, which serve to protect the microorganism from nitrosative stress within the intracellular environment. In this study, we demonstrate that expression of meningococcal NorB increases the rate at which low-molecular-weight S-nitrosothiol (SNO) decomposes in vitro. To determine whether this effect occurs in cells during infection by bacteria, we induced SNO formation in murine macrophages by activation with lipopolysaccharide and ?-interferon and observed a reduced abundance of SNO during coincubation with N. meningitidis, S. enterica, or E. coli. In each case, this effect was shown to be dependent on bacterial NO detoxification genes, which act to prevent SNO formation through the removal of NO. This may represent a novel mechanism of host cell injury by bacteria.—Laver, J. R., Stevanin, T. M., Messenger, S. L., Dehn Lunn, A., Lee, M. E., Moir, J. W. B., Poole, R. K., Read, R. C. Bacterial nitric oxide detoxification prevents host cell S-nitrosothiol formation: a novel mechanism of bacterial pathogenesis
System applications of holey fibers
No full textThe wavelength-scale features in holey fibers lead to novel properties including endlessly single-mode guidance, high optical nonlinearity. The current HF technology for a wide range of device applications in optical communication systems is reviewed
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