7,492 research outputs found
LN-5, but not LN-α2, was expressed in human epidermis.
No full textHuman skin was immunostained with an antibody against LN-α2 or LN-5. After washing with PBS, the skin samples were incubated with a mouse CyTM5- or a rabbit CyTM5-conjugated secondary antibody at room temperature for 2 h. Nuclei were counterstained for 5 min with DAPI. Scale bar: 20 μm.</p
Electronic band structure and photocatalytic activity of Ln(2)Ti(2)O(7) (Ln = La, Pr, Nd)
No full textPhotocatalytic activity in the water splitting of Ln(2)Ti(2)O(7) (Ln = La, Pr, Nd) with a layered structure was highly dependent on their electronic band structure. The conduction band of La2Ti2O7 consisted mainly of Ti 3d and La 5d, whereas the valence band consisted mainly of O 2p and Ti 3d. The empty La 4f level was found to be located ca. 3.6 eV above the bottom of the conduction band from both the electronic band-structure calculation and the XPS measurement. The occupied and unoccupied Ln 4f level in Ln(2)Ti(2)O(7) was shifted to lower energy as the number of 4f electrons increased. This shift of the Ln 4f band made it possible for the band-gap energy of both Pr2Ti2O7 and Nd2Ti2O7 to be decreased. For Nd2Ti2O7, the unoccupied Nd 4f level located between the conduction band and the valence band was found to be detrimental to photocatalytic activity in water splitting because it could act as an electron-trapping siteclose12012
Pedobacter panaciterrae sp nov., isolated from soil in South Korea
No full textA Gram-negative, facultatively anaerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated Gsoil 042(T), was isolated from a soil sample of a ginseng field in Pocheon province (South Korea) and was characterized taxonomically by using a polyphasic approach. The isolate was positive for catalase and oxidase, but negative for gelatinase and production of indole and H2S. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The major cellular fatty acids were summed feature 4 (C-16:1 omega 7c and/or iso-C-15:0 2-OH; 35.7%), iso-C-15:0 (24.3%) and iso-C-17:0 3-OH (11.6%). Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 042(T) fell within the radiation of the cluster comprising Pedobacter species. On the basis of 16S rRNA gene sequence similarities, the phylogenetically closest recognized species of the genus Pedobacter to strain Gsoil 042(T) included Pedobacter heparinus DSM 2366(T) (98.1%) Pedobacter africanus DSM 12126(T) (97.8%)and Pedobacter caeni LMG 22862(T) (97.2%) The 16S rRNA gene sequence similarity with other recognized species of the genus Pedobacter was less than 96.1%. DNA-DNA hybridization experiments showed that the DNA-DNA relatedness between strain Gsoil 042(T) and its phylogenetically closest neighbours was less than 43%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 042(T) should be classified as representing a novel species in the genus Pedobacter, for which the name Pedobacter panaciterrae sp. nov. is proposed. The type strain is Gsoil 042(T) (= KCTC 12594(T) = LMG 23400(T))
Lymphadenitis caused by non-tuberculous Mycobacterium in a university hospital in Taiwan: predominance of rapidly growing Mycobacteria and high recurrence rate
No full textMethylibium fulvum sp nov., a member of the Betaproteobacteria isolated from ginseng field soil, and emended description of the genus Methylibium
No full textTwo bacterial strains, designated Gsoil 322 T and Gsoil 328, were isolated from soil of a ginseng field in Pocheon province (Republic of Korea). The strains were Gram-negative, motile, aerobic rods that showed nearly identical physiological profiles and similar chemotaxonomic profiles. The two strains were oxidase-positive but catalase-negative, reduced nitrate to nitrite and had fatty acid profiles in which C-16:0, C-17:0 cyclo and C-16:10 omega 7c/iso-C-15:0 2-OH were predominant. The DNA G + C contents of Gsoil 322(T) and Gsoil 328 were 66.6 and 66.7 mol%, respectively. Q-8 was observed as the major quinone. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 322 T belongs to the class Betaproteobacteria and was most closely related to Methylibium petroleiphilum ATCC BAA-1232(T) (97.5% sequence similarity). On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 322(T) (=KCTC 12591(T) =LMG 23394(T)) was classified in the genus Methylibium as the type strain of a novel species, for which the name Methylibium fulvum sp. nov. is proposed
On the Linear Geometry of Lanthanide Hydroxide (Ln—OH, Ln=La-Lu)
No full textLanthanide hydroxides are key species in a variety of catalytic processes and in the preparation of corresponding oxides. This work explores the fundamental structure and bonding of the simplest lanthanide hydroxide, LnOH (Ln=La-Lu), using density functional theory calculations. Interestingly, the calculations predict that all structures of this series will be linear. Furthermore, these results indicate a valence electron configuration featuring an occupied sigma orbital and two occupied pi orbitals for all LnOH compounds, suggesting that the lanthanide-hydroxide bond is best characterized as a covalent triple bond
Serial analysis of fat-containing macrophages in bronchoalveolar lavage fluid in a patient with fat embolism syndrome
No full textPhenylobacterium koreense sp nov., isolated from South Korea
No full textT A Gram-negative, aerobic, rod-shaped, non-spore-forming bacterium, Slu-01(T) , was isolated from activated sludge from a wastewater treatment plant in Daejeon, South Korea. After 3 days on R2A medium, strain Slu-01(T) developed colourless colonies (0(.)-7-1(.)2 mm). The non-motile rods (0(.)7-1(.)0 x 1(.)0-2(.)0 mu m) were slightly curved and occurred singly and in pairs. No filamentous cells were found. On the basis of 16S rRNA gene sequence similarity, strain SlU-01(T) was shown to belong to the family Caulobacteraceae and was most closely related to Phenylobacterium immobile (96(.)6 %) and Phenylobacterium lituiforme (96(.)5 %). The genomic DNA G + C content of strain SlU-01(T) was 68(.)1 mol%, within the range of 67(.)3-68(.)4 mol% for the genus Phenylobacterium. Chemotaxonomic data (major ubiquinone, Q-10; major fatty acids C(18:1)omega 7c, C-16:0, C-15:0 and C-17:0) and 16S rRNA gene sequence analysis supported the affiliation of strain SlU-01(T) to the genus Phenylobacterium. However, DNA-DNA hybridization data and phenotypic properties showed that strain Slu-01(T) could be distinguished from the two other Phenylobacterium species with validly published names. Thus, it is suggested that strain Slu-01(T) represents a novel species of the genus Phenylobacterium, for which the name Phenylobacterium koreense is proposed. The type strain is SlU-01(T) (=KCTC 12206(T) = IAM 15119(T))
Methylobacterium jeotgali sp nov., a nonpigmented, facultatively methylotrophic bacterium isolated from jeotgal, a traditional Korean fermented seafood
No full textA novel facultatively methylotrophic, Gram-negative, rod-shaped bacterium, designated strain S2R03-9(T), was isolated from jeotgal, a traditional Korean fermented seafood. The organism was strictly aerobic, motile by means of a single polar flagellum, non-sporulating and catalase- and oxidase-positive. Strain S2RO3-9(T) grew in the presence of 0-1 % (w/v) NaCl and at pH 6.0-10.0, with optimum growth in the absence of NaCl and at pH 7.0. It grew at temperatures in the range 20.0-30.0 degrees C, with optimum growth at 30 degrees C. Colonies grown on R2A medium were nonpigmented, opaque and creamy white. Phylogenetic analysis based on 16S rRNA gene sequences indicated that it was most closely related to Methylobacterium organophilum JCM 2833(T) (96.6% similarity) and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 95.0%. The DNA G + C content was 64.9 mol%. The phylogenetic analysis, the phenotypic assessment and the chemotaxonomic data (major ubiquinone, Q-10; major fatty acids, C-18:1 and C-18:0) showed that S2RO3-9(T) represents a novel species within the genus Methylobacterium in the class Alphaproteobacteria, for which the name Methylobacterium jeotgali sp. nov. is proposed. The type strain is S2RO3-9(T) (= KCTC 12671(T) = LMG 23639(T))
Starkeya koreensis sp nov., isolated from rice straw
No full textA facultatively chemolithoautotrophic, Gram-negative, aerobic, highly curved rod-shaped, nonmotile, non-spore-forming bacterium, strain Jip08(T), was isolated from rice straw in Daejeon, South Korea. Strain Jip08(T) produced colourless colonies (1.0-1.5 mm) on R2A medium after 3 days. On the basis of 16S rRNA gene sequence similarity, strain Jip08(T) was shown to belong to the alpha-2 subclass of the Proteobacteria, and was most closely related to Starkeya novella IAM 12100(T) (98.6%), Angulomicrobium tetraedale DSM 5895(T) (98.1%), Angulomicrobium amanitiforme NCIMB 1785(T) (98.0%), Ancylobacter aquaticus ATCC 25396(T) (96.6%) and Methylorhabdus multivorans DM13(T) (95.8%), all of which belong to the family Hyphomicrobiaceae. The genomic DNA G + C content of strain Jip08(T) was 69 mol%. Chemotaxonomic data [major ubiquinone, Q-10; major fatty acids, C-18:1 isomer (summed feature of C-18:1 omega 7c/omega 9t/omega 12t), C-19:0 cyclo omega 8c and C-16:0], 16S rRNA gene sequence analyses and some morphological and physiological features supported the affiliation of strain Jip08(T) to the genus Starkeya. However, DNA-DNA hybridization data and some phenotypic properties showed that strain Jip08(T) could be distinguished from the single known Starkeya species and represented a novel species, for which the name Starkeya koreensis sp. nov. is proposed. The type strain is Jip08(T) (= KCTC 12212(T) = NBRC 100963(T) = IAM 15215(T))
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