23,617 research outputs found

    TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation

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    The interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification

    Lecture: Biographical and Musica Review, Robert Ch. Lee, April 1, 1963

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    Concert program for Lecture: Biographical and Musica Review, Robert Ch. Lee, April 1, 196

    Souvenir de Faust de Ch. Gounod : Op. 93 / Par S. Lee

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    SOUVENIR DE FAUST DE CH. GOUNOD : OP. 93 / PAR S. LEE Souvenir de Faust de Ch. Gounod : Op. 93 / Par S. Lee (1) Cover (1) Titelseite (3) Souvenir de Faust (4) Souvenir de Faust. Violincello (6

    A cure, a cure, a perfect cure, Oh, yes! a certain cure [first line of chorus]

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    strophic with choruspiano and voiceads on back cover for Lee & Walker stock8612 4Johns Hopkins University, Levy Sheet Music Collection, Box 047, Item 122Written by Ch. Sloman.As Sung By Frank Drew at the Philadelphia Academy of Music and Arch Street TheatreT. Sinclair's Lith. Phi

    A cure, a cure, a perfect cure, Oh, yes! a certain cure [first line of chorus]

    No full text
    strophic with choruspiano and voiceads on back cover for Lee & Walker stock8612 4Johns Hopkins University, Levy Sheet Music Collection, Box 047, Item 122Written by Ch. Sloman.As Sung By Frank Drew at the Philadelphia Academy of Music and Arch Street TheatreT. Sinclair's Lith. Phi

    Lincoln Quick Step

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    80.7568.667 – “Lincoln Quick Step”: Ch. Grobe: Lee & Walker: 1860: Piano Solo. (3 copies

    Geometric attack resistant watermarking in wavelet transform domain

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    In this paper, we propose an autocorrelation function (ACF) based watermarking scheme in the discrete wavelet transform (DWT) domain. Conventional ACF-based watermarking embeds a watermark in the spatial domain due to its detection mechanism. We show that the autocorrelation (AC) peaks, which play an important role in estimating the applied geometric attacks in ACF-based watermarking, can also be extracted by embedding the watermark in the DWT domain. In the proposed scheme, a periodic watermark is embedded in the DWT domain by considering the AC peak strength and noise visibility. The proposed scheme also deals efficiently with the image shift problem in the detection process by using the undecimated DWT. Experimental results show that the proposed scheme yields stronger AC peaks than the spatial domain scheme does and, as a result, shows improved robustness against combined geometric-removal attacks. (C) 2005 Optical Society of America

    Improved autocorrelation function based watermarking with side information

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    We propose an improved autocorrelation function (ACF)based image watermarking that is robust to combined geometric and removal attacks. ACF-based watermarking is thought of as one of the most effective watermarking schemes that resist geometric attacks. In this watermarking scheme, the autocorrelation peaks of the watermark play an important role for geometric attack estimation. The peaks, however, are vulnerable to attacks. The proposed scheme enhances the performance of ACF-based watermarking by improving the strength of the peaks. The information of an original image is used at the embedding time, so that the detector can extract strong autocorrelation peaks. Experimental results show that the proposed scheme yields better robustness than conventional ACF based watermarking against combined geometric-removal attacks. 2005 SPIE and IS&T

    Bidirectional WDM self-healing ring network based on simple bidirectional Add/Drop amplifier modules

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    We demonstrate a cost-effective bidirectional wavelength-division-multiplexed (WDM) self-healing ring network using a novel bidirectional add/drop amplifier module. The maximum power penalty incurred in this network for bidirectional transmission and add/drop multiplexing of WDM signals was measured to be less than 0.5 dB at an error rate of 10(-9). The restoration time was less than 2 ms. The results show that this network could accommodate at least ten nodes when the node spacing is 80 km
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