4,004 research outputs found
Pyrene-Modified Guanine Cluster Probes Forming DNA/RNA Hybrid Three-Way Junctions for Imaging of Intracellular MicroRNAs
No full textMicroRNAs (miRNAs) regulate gene expression in cells; high levels of expression are associated with various cancers. In this paper, we describe PyA-modified nucleic acid probes that can detect intracellular miRNAs by forming DNA/RNA hybrid three-way junction structures containing a fluorescent scaffold - a so-called G-cluster. This G-cluster featured two mismatched strands, four guanine residues, and one fluorescent adenine residue having a pyrene moiety covalently connected at the 8-position through an acetylene linker. The scaffold underwent a dramatic shift in its emission wavelength when two mismatched strands formed a duplex, similar to the behavior of an adenine pentad system (A-cluster). We applied the G-cluster scaffold in a three-way junction system to probe for miRNAs; its red-shifted fluorescence intensity and stability were greater than those reported previously for A-cluster three-way junction probes. Furthermore, confocal microscopy of cancer cell lines revealed bright fluorescence emissions in response to the miRNAs in the cells. Thus, this system can be applied intracellularly as a potential fluorescent probe for the detection of various biologically important nucleic acids. ?11Nscopu
Detection of AAG repeats through DNA triplex-induced G-cluster formation
No full textThis communication describes a novel method, using DNA triplex-based fluorescent probes, for the detection of AAG repeats. A triplex structure with target DNA AAG repeats stabilizes a (Py)A-modified G-cluster adjacent to the triplex-forming sequence, resulting in a dramatic change in the color of fluorescence from blue to orange.11Nsciescopu
A new strategy for in silico gene knock-out for strain improvement: Succinate production in E.coli as an example
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Transcriptome analysis of regulatory gene in sulfur containing amino acid biosynthesis, cysBin Escherichia coli
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Transcriptome profiling and deciphering the regulatory role of global transcription factor fadR
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