32 research outputs found
Differential expression and phenotypic activities of osterix in equine mesenchymal stem cell populations
No full textBone is one of the few tissues in vertebrates that possess the innate capacity for authentic regeneration, yet approximately 10% of fractures fail to heal adequately. There is considerable overlap in the cellular and molecular events occurring during embryological bone development and fracture repair. Mesenchymal stem cell (MSC) precursors undergo osteogenesis to give rise to osteoblasts, which are responsible for bone matrix synthesis and mineralization. Osteogenesis is regulated by two master transcription factors, runt-related transcription factor 2 (RUNX2) and Osterix (OSX), with interplay from signaling pathways such as the Bone Morphogenetic Protein (BMP) network. MSCs isolated from bone marrow (BM) and adipose tissue (ADI) are most commonly utilized in human and veterinary regenerative medicine applications. BM-MSCs have shown superior osteogenic capacity to ADI-MSCs in many studies, yet sample collection and culture techniques often vary between species. The overall objective of this dissertation was to determine factors that influence the in vitro osteogenic capacity differences of equine adult ADI- and BM-MSCs in order to standardize techniques and provide insights into avenues for improving osteogenesis in less-osteogenic MSC populations. The first study investigated the hypotheses that in vitro cell viability, proliferation, and osteogenic differentiation of MSCs collected from horses under general anesthesia and after euthanasia would be impaired compared to MSCs collected from horses during sedation. The second set of experiments characterized the RUNX2 and OSX response axes of ADI- and BM-MSCs during osteogenesis to test the hypotheses that 1) the relative osteogenic capacities are consistent with resting and inducible RUNX2 and OSX transcript levels, and 2) increasing OSX expression in ADI-MSCs improves their osteogenic capacity. The third series of experiments evaluated the comparative BMP signaling activities in ADI- and BM-MSCs to determine whether 1) osteogenic BMP ligand expression differs, 2) BMP inhibition in BM-MSCs impairs osteogenesis, and 3) BMP supplementation to ADI-MSCs improves osteogenesis. The results indicate that donor status during MSC collection does not affect in vitro cell viability, proliferation, and osteogenic capacity. BM-MSCs have higher OSX expression and intrinsic BMP signaling in both resting and osteogenic conditions than ADI-MSCs. Endogenous BMP activity is necessary for osteogenesis in BM-MSCs, and exogenous BMP supplementation, but not OSX over-expression, was sufficient to improve the osteogenic capacity of ADI-MSCs. In summary, OSX expression and BMP signaling significantly influence the in vitro osteogenic capacity of MSC populations.Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2020-12-01The student, Kalyn Herzog, accepted the attached license on 2018-11-02 at 16:55.The student, Kalyn Herzog, submitted this Dissertation for approval on 2018-11-02 at 16:58.This Dissertation was approved for publication on 2018-11-06 at 12:51.DSpace SAF Submission Ingestion Package generated from Vireo submission #13055 on 2019-02-08 at 11:38:22Made available in DSpace on 2019-02-08T18:39:43Z (GMT). No. of bitstreams: 2
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Previous issue date: 2018-11-06Embargo set by: Seth Robbins for item 109928
Lift date: 2021-02-08T18:40:00Z
Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 109928
Lift date: 2021-02-08T18:42:23Z
Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 109928
Lift date: 2021-02-08T18:43:54Z
Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemEmbargo set by: Seth Robbins for item 109928
Lift date: 2021-02-08T18:44:50Z
Reason: Author requested closed access (OA after 2yrs) in Vireo ETD systemLimited Restriction Lifted for Item 109928 on 2021-02-09T10:15:12Z
Giving voice to ideas : the role description plays in the diffusion of radical innovations
No full textThesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Urban Studies and Planning, 1999.Includes bibliographical references (leaves 134-144).One of the more stable findings in the "diffusion and knowledge utilization" literature is that simple innovations. those compatible with the existing practices in a field. are spread more easily than those which challenge standard practice. Yet it is the more radical innovations that hold special promise for advancing the practice of a field. Using an action research methodology. the author studied the diffusion of radical innovations in two very different programmatic settings. first in an undergraduate affirmative action program on a university campus and later in a philanthropic-driven effort to fund charitable work with recoverable investments rather than grants--a practice that is called "program-related Investing." The two programs together served as test cases--one as a precipitating paradox and the other as a conscious experiment--in overcoming barriers to the diffusion of an important category of innovations: innovations that require individuals to practice in new ways and acquire new skills, that cause some disruption to the broader organization and that involve the "soft" technologies of knowledge rather than the "hard" material technologies. The literature treated diffuser's descriptions of their innovations as self-evident. whereas the author found that diffusers of these radical. practice innovations unintentionally gave incomplete and in some cases misleading descriptions of their work. An argument is made that effective description must do more than represent the original innovation with some accuracy. It must enable diffusers to teach those aspects of their practice which am difficult for them to make explicit by including opportunities for practicing side-by -side. whether these be through simulated practice worlds or actual ones. It must also enable appropriate transformation of the innovation. This can be best accomplished by structuring a dialogue between diffusers and (potential) users to lift up multiple descriptions of the practice. It is the process of comparing such descriptions that allows diffusers and users to build up an understanding both of the essence of the innovation and of ways in which transformations may preserve or damage this essence.by Kalyn Culler Cohen.Ph.D
Wasa Lake
No full textGeospatial Technologies & Natural Resource ManagementColumbia Basin Watershed Networ
Trauma informed care in nursing: A literature review
No full textTrauma-informed care (TIC) Is a commonly used term without a universal definition.
Patient care should be approached in a way that does not induce or reactivate any past trauma.
Recognizing patients' past trauma will allow providers to deliver patient-centered care and create a safe environment.
The goal of this review is lo clarify the borrowed concept of TIC in the field of nursing.
TIC may contribute to improving health outcomes
Glade Watershed
No full textGeospatial Technologies & Natural Resource ManagementColumbia Basin Watershed Networ
Mather Creek (Mainstreems)
No full textGeospatial Technologies & Natural Resource ManagementColumbia Basin Watershed Networ
Collections of Disorder: Stories of Mental Illness
No full textThis thesis contains five short stories, each narrated by a character with a psychological disorder. The disorders represented are as follows: alcohol use disorder, schizophrenia, Alzheimer’s disease, phobic disorder, and autism spectrum disorder. Research was conducted in two parts: (1) study of psychological texts including peer reviewed articles and case studies and (2) study of literary works including memoirs and novels. The author aims to use storytelling to humanize and therefore generate empathy for those with mental illnesses
Collections of Disorder: Stories of Mental Illness
No full textThis thesis contains five short stories, each narrated by a character with a psychological disorder. The disorders represented are as follows: alcohol use disorder, schizophrenia, Alzheimer’s disease, phobic disorder, and autism spectrum disorder. Research was conducted in two parts: (1) study of psychological texts including peer reviewed articles and case studies and (2) study of literary works including memoirs and novels. The author aims to use storytelling to humanize and therefore generate empathy for those with mental illnesses.Bachelor of Arts (BA)Englis
Citation analysis based on Google Scholar
No full textGoogle Scholar is an increasingly popular search engine with the largest bibliographic database for citation analysis that is accessible to anyone unlike other bibliographic databases which may incur monthly fees. With the increase of publications of scholarly papers, there is an increase in demand for quality measurement of papers for assessing the author’s publication works. The evaluations of author’s publications rely on citation analysis as to achieve an unbiased evaluation of an author’s work. H-index is one of the mostly used citation metric, thus, two unique additional calculations of h-index with unknown authors’ citations per paper and citations without self-citation of the author were included and compared against the original h-index calculation in this report. Hence, this report presents the study of the citation analysis of citation records based on Google Scholar.
A python parser tool was implemented to parse information from Google Scholar based on three author name searches namely: “Xiaokui Xiao”, “Yunhao Liu” and “Mong LiLee”. Three different h-index versions were calculated and the results were analyzed. The results have shown that a relatively high self-citation by the authors have significant contribution to the overall citation counts that may affect the h-index value, leading to a slight increase in the value of h-index. However, a small value of self-citation does not affect the h-index value. When comparing the two unique h-index versions, version 2 and 3, version 2 has more impact on the overall value of the h-index. Overall, author “Yunhao Liu” has achieved the highest citation counts and the highest h-index value among the other authors, indicating that its publications have high significant contribution, quality and impact to the other authors.
In addition, Google Scholar has limitation such as displaying issues, blockage problems and non-scholarly sources that may distort citation metrics. Thus, there are pros and cons when using Google Scholar as citation analysis source. Hence, the citation statistic results obtained from Google Scholar for the different authors reflect the significance and the impact of the authors ’publications. The higher the total numbers of citation, the better the h-index, the higher impact and quality of the publication papers of the authors’.Bachelor of Engineering (Computer Science
