85 research outputs found
The Oratione alli fuoriusciti di Fiorenza et altri cittadini amatori della libertà (1556). For an attribution to Bartolomeo Cavalcanti
No full textThe essay gives a new contribution to the study of forentine political exile through the publication and the analysis of an unpublished text: the oratione alli fuorusciti di Fiorenza et altri cittadini amatori della libertà. Almost forgotten by historians, the document proves the vitality of the republican spirit. After the truce of Vaucelles and despite the recent defeats in war (Marciano and Siena), it in fact shows that the hope of disengaging from the tyranny of the Medici family was still in the exiles' environment. According to a series of cultural, rhetorical and ideological elements the suggestive hypothesis that the author of the text is Bartolomeo Cavalcanti is brought forward. Bartolomeo Cavalcanti was a famous literary figure and diplomat and one of the main Florentine Diaspora representatives. In those years he was living in Rome on behalf of the Farnese family and would shortly be entering the service of cardinal François de Tournon
Supplemental Material, Supplementary_Tables_Final_April_6_2020 - Resident-Level Predictors of Dementia Pharmacotherapy at Long-Term Care Admission: The Impact of Different Drug Reimbursement Policies in Ontario and Saskatchewan
No full textSupplemental Material, Supplementary_Tables_Final_April_6_2020 for Resident-Level Predictors of Dementia Pharmacotherapy at Long-Term Care Admission: The Impact of Different Drug Reimbursement Policies in Ontario and Saskatchewan by Laura C. Maclagan, Susan E. Bronskill, Michael A. Campitelli, Shenzhen Yao, Christoffer Dharma, David B. Hogan, Nathan Herrmann, Joseph E. Amuah and Colleen J. Maxwell in The Canadian Journal of Psychiatry</p
Supplemental Material, Appendix_1_Final_April_6_2020 - Resident-Level Predictors of Dementia Pharmacotherapy at Long-Term Care Admission: The Impact of Different Drug Reimbursement Policies in Ontario and Saskatchewan
No full textSupplemental Material, Appendix_1_Final_April_6_2020 for Resident-Level Predictors of Dementia Pharmacotherapy at Long-Term Care Admission: The Impact of Different Drug Reimbursement Policies in Ontario and Saskatchewan by Laura C. Maclagan, Susan E. Bronskill, Michael A. Campitelli, Shenzhen Yao, Christoffer Dharma, David B. Hogan, Nathan Herrmann, Joseph E. Amuah and Colleen J. Maxwell in The Canadian Journal of Psychiatry</p
Phylogenetic analysis of the surface glycoprotein genes of human type B Italian influenza isolates after the reemergence in 2001 of B/Victoria/2/87-lineage viruses
No full textTwo major lineages of influenza B viruses have circulated in humans, the B/Yamagata/16/88 (Yam88)-lineage and the B/Victoria/2/87 (Vic87)-lineage. Throughout most of the last decade, Yam88-lineage viruses predominated. During the 2001–2002 influenza season, however, Vic87-lineage viruses emerged outside Asia and spread worldwide. Virological surveillance in Italy during this season highlighted a predominance of influenza B viruses, most of which were antigenically related to the B/Sichuan/379/99 vaccine strain, belonging to the Yam88-lineage, together with a smaller number of B viruses antigenically similar to a recent Vic87-lineage variant B/Hong Kong/330/01. Vic87-lineage viruses were exclusively isolated in the subsequent 2002–2003 epidemic season. Phylogenetic analyses of the hemagglutinin (HA) and neuraminidase (NA) genes of representative Italian B isolates from the 2001–2002 and 2002–2003 seasons showed that, during the first season, the NA as well as the HA genes separated into two distinct clades, the Yam88- and the Vic87-lineages and showed no evidence of reassortment. On the contrary, all the B viruses isolated in the 2002–2003 epidemic season were “Vic87 HA–Yam88 NA” reassortants similar to those isolated in other parts of the world, showing that these reassortants became well-established in the human population
Aristotle-Aquinas metaphysic valuation’s concerning the access to Justice
No full textA presente dissertação tem por objetivo empreender uma análise do princípio constitucional do acesso à justiça à luz da metafísica e da física aristotélico-tomista. O método utilizado serviu-se de levantamento bibliográfico, análise, síntese e crítica do modelo jurídico atual do ponto de vista da filosofia do direito. Baseado nas contribuições de Mauro Cappelleti e Bryant Garth, que fizeram histórico relatório sobre o tema, encontrou-se dois significados distintos em sua interpretação, concentrando-se este texto no segundo, qual seja: produção de decisões justas para o indivíduo e sociedade. Resgatou-se em Aristóteles e Tomás de Aquino as origens desse pensamento. Fez-se uma análise da doutrina da justiça à luz do direito natural aristotélico e do tratado da caridade em Tomás de Aquino e situada possibilidade do acesso à justiça como um abrir-se essencial à interpretação e universalização do acesso. Esse movimento de abertura foi visto à luz da componente aitiológica da metafísica. Concluiu-se que através da reabilitação da metafísica aristotélico-tomista aplicada ao direito, pode-se potencializar o acesso à justiça e a efetividade do direito tendo o homem como fundamento, sustentação e fimThis dissertation aims to undertake a metaphysic analysis about the constitutional right of the access to justice supported on the doctrines of Aristotle and St. Thomas of Aquin. The methodology used by the author was about bibliographic researchs and the analysis, the synthesis, and the critique applied to the actual stage of comprehension of the legal phenomenon from the perspective of the Philosophy of Law. From the contributions of Mauro Cappelleti and Bryant Garth, contained in their relatory about the access to justice, the author have found two different points of view and heading for the second: the production of just and effectiveness decisions to the people and to the society. The author’s research lead to the conceptions of Metaphysic and Physic in Aristotle’s Philosophy as the original source of this possibility exposed by Mr. Chiappin, as well as in Charitas doctrine in St. Thomas as possibility of universalization of the doctrine of the four causes that covers all the mankind, gathered under the label of natural law. The opening that this analysis provided, lead the author to a brand new level of understanding about the access to justice, which was examined under the aitiologic component of the aristotelic metaphysics. The conclusion was that the rehabilitation of the aristotelic-thomist way of think can magnify the men as the ground, the support and the end of the law through access to justiceConselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNP
Uso de Embriões em Testes de Toxicidade de Herbicidas, uma Revisão Abrangente
No full textThe use of embryos as an alternative model to assess herbicide toxicity stands out for its importance in reducing the use of animals in toxicological studies and in aligning with the ethical principles of the 3Rs. The use of herbicides in agriculture raises concerns about their environmental and public health risks and, therefore, embryonic models of birds, amphibians, zebrafish and mice emerge as sensitive and ethical tools to identify the toxic effects of these compounds on development. This study aimed to qualitatively review articles that explore the toxicity of herbicides using embryonic models. Searches conducted in databases such as ScienceDirect and PubMed focused on studies examining compounds including Glyphosate, 2,4-D, Atrazine, Diuron, S-metolachlor, Acetochlor, and Pendimethalin. The findings reinforce the effectiveness of embryonic models in detecting toxic effects at low concentrations, highlighting their sensitivity and utility for toxicological evaluations. However, the study also identified challenges, particularly the lack of methodological standardization and the diversity of approaches used in the analyzed studies. These limitations hinder the generalization and comparison of results, complicating a comprehensive understanding of the toxic potential of these substances. Despite these challenges, the use of embryos in agrochemical testing demonstrates potential to replace traditional animal testing. It offers a more ethical and replicable methodology, with the possibility of developing standardized protocols that could significantly contribute to a deeper understanding of herbicides' impacts on health and the environment.Trabalho de Conclusão de Curso (Graduação)O uso de embriões como modelo alternativo para avaliar a toxicidade de herbicidas, destaca-se por reduzir o uso de animais em estudos toxicológicos e se alinhar aos conceitos éticos dos 3R’s. Sabe-se que a utilização de herbicidas na agricultura levanta preocupações acerca de seus riscos ambientais e de saúde pública e, com isso, os modelos embrionários de aves, anfíbios, zebrafish e camundongos emergem como ferramentas sensíveis e éticas para identificar os efeitos tóxicos desses compostos no desenvolvimento. Assim, o objetivo desse estudo foi revisar, qualitativamente, artigos que verificam a toxicidade de herbicidas utilizando esse modelo. A separação desses estudos foi feita por meio de bases de dados como ScienceDirect e PubMed. Dessa forma, foram selecionados artigos que testavam os princípios ativos mais utilizados na agricultura como Glifosato, 2,4-D, Atrazina, Diuron, S-metolachlor, Acetochlor e Pendimetalina. Os resultados encontrados reforçam a sensibilidade do modelo embrionário na detecção de efeitos tóxicos em baixas concentrações, se mostrando, assim, uma ferramenta eficaz para esse tipo de estudo. Pontanto, apesar da falta de padronização metodológica e a heterogeneidade dos estudos limitarem a generalização, a comparação e o real entendimento do potencial tóxico das substâncias, o uso de embriões em testes de agroquímicos possui potencial para substituir os testes em animais e avançar no entendimento dos impactos de herbicidas na saúde, pois se trata de uma metodologia mais ética, replicável e que viabiliza a criação de protocolos uniformes que poderão contribuir para obtenção de mais dados sobre esse tema
The Function and Evolution of C2H2 Zinc Finger Proteins and Transposons
Get PDFTranscription factors (TFs) confer specificity to transcriptional regulation by binding specific DNA sequences and ultimately affecting the ability of RNA polymerase to transcribe a locus. The C2H2 zinc finger proteins (C2H2 ZFPs) are a TF class with the unique ability to diversify their DNA-binding specificities in a short evolutionary time. C2H2 ZFPs comprise the largest class of TFs in Mammalian genomes, including nearly half of all Human TFs (747/1,639). Positive selection on the DNA-binding specificities of C2H2 ZFPs is explained by an evolutionary arms race with endogenous retroelements (EREs; copy-and-paste transposable elements), where the C2H2 ZFPs containing a KRAB repressor domain (KZFPs; 344/747 Human C2H2 ZFPs) are thought to diversify to bind new EREs and repress deleterious transposition events. However, evidence of the gain and loss of KZFP binding sites on the ERE sequence is sparse due to poor resolution of ERE sequence evolution, despite the recent publication of binding preferences for 242/344 Human KZFPs. The goal of my doctoral work has been to characterize the Human C2H2 ZFPs, with specific interest in their evolutionary history, functional diversity, and coevolution with LINE EREs. I contributed to the expert curation of the full complement of 1,639 Human TFs and used the results to quantitatively compare their evolutionary history and tissue specificities to all other Human TFs. I analyzed protein-protein interaction (PPI) data for 118 DNA-binding C2H2 ZFPs and found extremely diverse interactions with nuclear factors despite paradoxically few dedicated PPI domains, revealing a new and unexplained dimension of functional diversity in addition to DNA-binding specificity diversity. Finally, I pioneered a computational technique to reconstruct extinct LINE L1 sequences and showed that they can be used to anchor the integration of KZFP genomic binding data and binding specificities for a complete picture of dynamic KZFP-ERE sequence specificity relationships. Together, my results paint a detailed picture of the diverse functionality and rapid evolution of Human C2H2 ZFPs, contribute to ongoing theorization on KZFP-ERE coevolution, and provide parallel datasets to power future investigations.Ph.D
Una Madonna dendrofora a Cagliari?
No full textNella moderna chiesa parrocchiale del quartiere La Palma in Cagliari si conserva un bel dipinto devozionale: di iconografia mariana, ancora inedito, di cui si ignora paternità ed epoca di realizzazione. Per quanto concerne l’iconografia, si propone la sua derivazione da un antico e venerato prototipo romano, l’icona di S. Maria in Portico detta Portus Romanae Securitatis, oggi conservata in S. Maria in Campitelli. Preso in esame dal punto di vista storico-artistico, per quanto concerne la sua datazione si attribuisce ad ambito controriformato romano dei primi del ‘600, vicino alla cerchia di Giuseppe Valeriano e Scipione Pulzone o dei loro seguaci.In the modern parish church of La Palma quarter, in Cagliari, is kept a beautiful devotional painting, a Marian icon, still unpublished. The author and period of realization are unknown. As regards iconography, the painting may derive from an old and venerated Roman prototype, the S. Maria in Portico icon, known as Portus Romanae Securitatis, kept at present in S. Maria in Campitelli. From an artistic, historical point of view, the dating of the picture could be ascribed to the first decades of the seventeenth century, during the Roman Counter Reformation period. The painting may be attributed to Giuseppe Valeriano and Scipione Pulzone school or to some of their followers
Pluralismo e crise de sentido no sistema jurídico
No full textEste trabalho busca contribuir com a discussão a respeito da descrição do sentido das
comunicações sociais, proposto por Niklas Luhmann, a partir da identificação de uma
dimensão espacial de sentido, ao lado das dimensões objetiva, social e temporal já
diagnosticadas pelo autor. Vale-se, para tanto, da interseção das análises de Harisson C.
White, Ignácio Farías e Hugo Cadenãs para formular o conceito de “mundos plurais”,
entendidos como níveis intermediários entre as interações e os sistemas sociais, que surgem
em razão da performação de elementos sociomateriais, narrativas, estilos e valores comuns.
Luhmann não considerou a formação de ‘grupos’ dentro de seu esquema de planos de
diferenciação social, destacando somente as interações, organizações e sociedade, e incluindo,
apenas tardiamente, os movimentos de protesto. Do mesmo modo, ao tratar da ‘cultura’
considerou-o como um tema demasiado amplo, inviável à observação social.
Contudo, acredita-se que, ao se reformular o conceito de cultura, é possível observar outros
planos de diferenciação social que interagem com os sistemas funcionais, ao demarcarem
‘zonas de comunicação’ que atuam na formação das escolhas sistêmicas. Apesar de não serem
sistemas funcionais, os “mundos plurais” formados pela distinção cultural compartilham
importantes características de outros sistemas sociais, como a estruturação de um meio
próprio de generalização simbólica capaz de potencializar as chances de êxito das
comunicações sistêmicas ao reforçar o lado da forma familiar para as comunicações que
espelhem o mesmo padrão de sentidos por eles selecionados.
Entende-se, assim, que os “mundos plurais” formados pela cultura e os sistemas sociais
encontram-se conectados por contextos comunicativos particulares que ativam formas
imunizantes de crises sistêmicas episódicas decorrentes da clausura cognitiva pela reprodução
compulsiva de feedbacks positivos anteriores.
Especificamente quanto ao sistema jurídico, os “mundos plurais” permitem o equilíbrio entre
estabilidade e instabilidade, mudança e conservação; e mais, por darem origem ao metacódigo
da cultura, permitem a interpenetração entre as sistemas funcionais e sistemas psíquicos ao
chamar as consciências à aceitação de suas ofertas comunicativasThis study propose to contribute with the discussion about the description of the meaning of
social communications, in the way as proposed by Niklas Luhmann, from the identification of
a spatial dimension of meaning, alongside the objective, social and temporal dimensions
already diagnosed by the author.
It uses, therefore, intersection of Harisson C. White, Ignácio Farías e Hugo Cadenãs’s
analyzes to formulate the concept of "plural worlds," understood as intermediate levels
between interactions and social systems, arising from the performation of common
sociomaterial elements, narratives, styles, and values.
Luhmann did not consider the formation of 'groups' within his scheme of social differentiation
plans, highlighting only interactions, organizations and society, and including, only belatedly,
protest movements. Likewise, in dealing with the theme of 'culture' he considered it as too
broad a theme, unfeasible to social observation.
However, this study sustain that in reformulating the concept of culture, it becomes possible
to observe other plans of social differentiation that interact with the functional systems, by
demarcating 'communication zones' that act in the formation of the systemic choice.
Although they are not functional systems, the "plural worlds" formed by the cultural
distinction share important characteristics of other social systems, such as the structuring a
“medium of symbolic generalization” capable of enhancing the chances of success of
systemic communications by reinforcing the form side familiar for the communication that
resembles the same pattern of senses they select.
It is understood, therefore, that the "plural worlds" formed by culture and social systems are
connected by particular communicative contexts that activate immunizing forms of episodic
systemic crises arising from the cognitive closure by the compulsive reproduction of previous
positive feedbacks.
Specifically regarding the legal system, the "plural worlds" allow the balance between
stability and instability, change and conservation; and more, by giving rise to the metacode of
culture, allow the interpenetration between functional systems and psychic systemsCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPE
A sensitive one-step real-time PCR for detection of avian influenza viruses using a MGB probe and an internal positive control
Get PDFBackground: Avian influenza viruses (AIVs) are endemic in wild birds and their introduction and conversion to
highly pathogenic avian influenza virus in domestic poultry is a cause of serious economic losses as well as a risk
for potential transmission to humans. The ability to rapidly recognise AIVs in biological specimens is critical for
limiting further spread of the disease in poultry. The advent of molecular methods such as real time polymerase
chain reaction has allowed improvement of detection methods currently used in laboratories, although not all of
these methods include an Internal Positive Control (IPC) to monitor for false negative results.
Therefore we developed a one-step reverse transcription real time PCR (RRT-PCR) with a Minor Groove Binder
(MGB) probe for the detection of different subtypes of AIVs. This technique also includes an IPC.
Methods: RRT-PCR was developed using an improved TaqMan technology with a MGB probe to detect AI from
reference viruses. Primers and probe were designed based on the matrix gene sequences from most animal and
human A influenza virus subtypes. The specificity of RRT-PCR was assessed by detecting influenza A virus isolates
belonging to subtypes from H1–H13 isolated in avian, human, swine and equine hosts. The analytical sensitivity of
the RRT-PCR assay was determined using serial dilutions of in vitro transcribed matrix gene RNA. The use of a
rodent RNA as an IPC in order not to reduce the efficiency of the assay was adopted.
Results: The RRT-PCR assay is capable to detect all tested influenza A viruses. The detection limit of the assay
was shown to be between 5 and 50 RNA copies per reaction and the standard curve demonstrated a linear range
from 5 to 5 × 108 copies as well as excellent reproducibility. The analytical sensitivity of the assay is 10–100 times
higher than conventional RT-PCR.
Conclusion: The high sensitivity, rapidity, reproducibility and specificity of the AIV RRT-PCR with the use of IPC
to monitor for false negative results can make this method suitable for diagnosis and for the evaluation of vira
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