2,571 research outputs found
A combination of molecular cytogenetic analyses reveals complex genetic alterations in conventional renal cell carcinoma
No full textHere we report the complex pattern of genomic imbalances and rearrangements in a panel of 19 renal cell carcinoma cell lines detected with molecular cytogenetic analysis. Consistent heterogeneity in chromosome number was found, and most cell lines showed a near-triploid chromosome complement. Several cell lines showed deletions of the TP53 (alias p53), CDKN2A (alias p16), and VHL genes. Multiplex fluorescence in situ hybridization (M-FISH) analysis revealed chromosome 3 translocated to several other partners chromosomes, as well as breakage events commonly affecting chromosomes 1, 5, 8, 10, and 17. The most common abnormality detected with comparative genomic hybridization (CGH) was deletions of chromosome 3p, with loss of the RASSF1, FHIT, and p44S10 loci frequently involved. CGH gain of 5q showed overrepresentation of the EGR1 and CSF1R genes. Recurrent alterations to chromosome 7 included rearrangement of 7q11 and gains of the EGFR, TIF1, and RFC2 genes. Several lines exhibited rearrangement of 12q11q14 and overrepresentation of CDK4 and SAS loci. M-FISH revealed several other recurrent translocations, and CGH findings included loss of 9p, 14q, and 18q and gain of 8q, 12, and 20. Further genomic microarray changes included loss of MTAP, IGH@, HTR1B, and SMAD4 (previously MADH4) and gains of MYC and TOP1. An excellent correlation was observed between the genomic array and FISH data, demonstrating that this technique is effective and accurate. The aberrations detected here may reflect important pathways in renal cancer pathogenesis
SDS-PAGE analysis of purified TIM, ALD, FBP and PGI.
No full text<p>Lane M, marker; lane 1, the soluble fraction of the <i>E. coli</i> cell lysate containing TIM; lane 2, purified TIM; lane 3, the soluble fraction of the <i>E. coli</i> cell lysate containing; lane 4, purified ALD; lane 5, the soluble fraction of the <i>E. coli</i> cell lysate containing FBP; lane 6, purified FBP; lane 7, the soluble fraction of the <i>E. coli</i> cell lysate containing PGI; and lane 8, purified PGI.</p
Opportunities for linking young surveyors across professional surveying member organisations and FIG
No full textLane-specific speed analysis in urban work zones with computer vision
No full textWork zone speed is one of the most important factors in road construction safety management. This work presents a computer vision based technique designed to measure lane-specific individual vehicle speed using existing traffic monitoring cameras and computers. The resulted speeds support the influence analysis of factors including traffic control, lane positions, and construction activity. Object detection (YOLOv5) and tracking (Deep-SORT) algorithms are combined to track the vehicles. In particular, 21 days’ worth of road construction videos are collected from a pole-mounted traffic monitoring camera operated by the Texas A&M University Transportation Services. Based on the object detection results, a novel construction activity inference technique is developed to approximate the times when construction workers are present. Based on this time separation, the vehicle speeds with and without the presence of construction activity are compared. The proposed framework is able to measure speeds with an error ranging from 0 to 6.4 kilometers per hour (KPH). Detailed analysis of this video data suggests that traffic control with barrels in the median work zone lowers the average speed (for all vehicles) by 15 KPH. The lane adjacent to the work zone also has higher speed variation than the other lanes. The construction activity speed comparisons show when the traffic is slow (possibly traffic after a red light), the difference is statistically significant with a p-value ranging from 0.01 to 0.03. When the traffic is fast (possibly traffic encountering a green signal as they approached the nearby intersection) construction activity has no significant effect on the work zone speeds. The proposed CV technique is a reliable and cheap method to measure lane-specific work zone speeds. The derived measurements support detailed safety analysis. Other than work zone speeds, the proposed technique can also be used for regular traffic speed monitoring.</p
Children\u27s/Young Adult (YA) Author Event: Tim Green Author Visit
No full textThe Children’s/Young Adult Author Committee at Olivet Nazarene University received a $2500 Community Engagement Grant from the university. Because of this grant, the university hosted Tim Green, a former NFL football player who is now authoring books of primary interest to fourth through eighth graders. The success of this grant is difficult to measure, but in numbers, more than 3200 4th - 8th grade students and their teachers attended his speaking events during his two day visit. Green autographed more than 400 books for the attendees. Regarding reading motivation, area teachers have and still are reporting students, boys in particular, who in the past have never read a whole book, but when the teachers give them one of Tim Green’s books, they return and ask for more of his books to read. The Children’s/Young Adult Author Committee plans to continue bringing authors of quality literature to the community
Novel chromosome findings in bladder cancer cell lines detected with multiplex fluorescence in situ hybridization
No full textBladder cancer is a common neoplasm worldwide, consisting mainly of transitional cell carcinomas, while squamous, adenocarcinoma, and sarcomatoid bladder cancers account for the remaining cases. In the present study, multiplex fluorescence in situ hybridization (M-FISH) has been used to characterize chromosome rearrangements in eight transitional and one squamous cell carcinoma cell line, RT112, of UMUC-3, 5637, CAT(wil), FGEN, EJ28, J82, 253J, and SCaBER. Alterations of chromosome 9 are the most frequent cytogenetic and molecular findings in transitional cell carcinomas of all grades and stages, while changes of chromosomes 3, 4, 8, 9, 11, 14, and 17 are also frequently observed. In the present study, alterations previously described, including del(8)(p10), del(9)(p10), del(17)(p10), and overrepresentation of chromosome 20, as well as several novel findings, were observed. These novel findings were a del(15)(q15) and isochromosome 14q, both occurring in three of nine cell lines examined. These abnormalities may reflect changes in bladder tumor biology. M-FISH represents an effective preliminary screening tool for the characterization of complex tumor karyotypes
Evaluating Citebase, an open access Web-based citation-ranked search and impact discovery service
No full textCitebase is a new citation-ranked search and impact discovery service that measures citations of scholarly research papers which are openly accessible on the Web, i.e. papers that are assessable continuously online. Other services, such as ResearchIndex, have emerged in recent years to offer citation indexing of Web research papers. In the first detailed user evaluation of an open access Web citation indexing service, Citebase has been evaluated by nearly 200 users from different backgrounds. The paper details the procedures used in the evaluation, and analyses the results of this study, which took place between June and October 2002. It was found that within the scope of its primary components, the search interface and services available from its rich bibliographic records, Citebase can be used simply and reliably for the purpose intended, and that it compares favourably with other bibliographic services. It is shown tasks can be accomplished efficiently with Citebase regardless of the background of the user. More data need to be collected and the process refined before it is as reliable for measuring citation impact of indexed papers. Better explanations and guidance are required for first-time users. Coverage is seen as a limiting factor, even though Citebase indexes over 200,000 papers from arXiv. Non-physicists were frustrated at the lack of papers from other sciences. The principle of citation searching of open access archives has thus been demonstrated and need not be restricted to current users. Since the evaluation, Citebase has become a featured service of the ArXiv physics eprint archives
Towards Protecting Critical Infrastructure - The Role of Information Security Management in Australian Universities
No full textCatholic Comments Podcast.
No full textAuthor Tim Rinaldi discusses his mission work in Honduras and how it changed his life and perspective
Accepting Optimally in Automated Negotiation with Incomplete Information (abstract)
No full textIntelligent SystemsElectrical Engineering, Mathematics and Computer Scienc
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