2,223 research outputs found
Identification of ANG-binding DNA fragments.
No full text<p>(<b>A</b>) Schematic illustration of ChIP screen of ANG-binding DNA. (<b>B</b>) Sonicated chromatin samples from HeLa cells were immunoprecipitated overnight with ANG antibody or IgG and applied to Western blot analysis. Data showed specific enrichment of ANG in the antibody group.</p
Angiotensin II induces soluble fms-Like tyrosine kinase-1 release via calcineurin signaling pathway in pregnancy
No full textMaternal endothelial dysfunction in preeclampsia is associated with increased soluble fms-like tyrosine kinase-1 (sFlt-1), a circulating antagonist of vascular endothelial growth factor and placental growth factor. Angiotensin II (Ang II) is a potent vasoconstrictor that increases concomitant with sFlt-1 during pregnancy. Therefore, we speculated that Ang II may promote the expression of sFlt-1 in pregnancy. Here we report that infusion of Ang II significantly increases circulating levels of sFlt-1 in pregnant mice, thereby demonstrating that Ang II is a regulator of sFlt-1 secretion in vivo. Furthermore, Ang II stimulated sFlt-1 production in a dose- and time-dependent manner from human villous explants and cultured trophoblasts but not from endothelial cells, suggesting that trophoblasts are the primary source of sFlt-1 during pregnancy. As expected, Ang II-induced sFlt-1 secretion resulted in the inhibition of endothelial cell migration and in vitro tube formation. In vitro and in vivo studies with losartan, small interfering RNA specific for calcineurin and FK506 demonstrated that Ang II-mediated sFlt-1 release was via Ang II type 1 receptor activation and calcineurin signaling, respectively. These findings reveal a previously unrecognized regulatory role for Ang II on sFlt-1 expression in murine and human pregnancy and suggest that elevated sFlt-1 levels in preeclampsia may be caused by a dysregulation of the local renin/angiotensin system
Immunohistochemistry staining of ANG and ERRγ in breast tissue samples.
No full text<p>Tissue microarray slide containing human breast ductal carcinoma tissues (<b>A, B</b>) and adjacent normal breast tissues (<b>C, D</b>) were stained with ANG (<b>A, C</b>) or ERRγ (<b>B, D</b>) antibody and visualized with Dako’s Envision kit. (Magnification: ×100). (<b>E</b>) The roles of ANG in the nucleus. ANG in the nucleolus promotes rRNA production and ribosome biogenesis. Meanwhile, ANG in the nucleoplasm regulates the expressions of its target genes to regulate cancer cell proliferation coordinately.</p
ANG regulates the repressive activity of ABSE and ERRγ expression.
No full text<p>(<b>A</b>) Schematic illustration of plasmids used in the luciferase assays. (<b>B</b>) MCF-7 cells were transfected with the indicated plasmids together with siRNAs targeting control or ANG. Luciferase activities were detected 48 hours after transfection. Relative luciferase activity is a ratio of Firefly luciferse units normalized to Renilla luciferase units. (<b>C</b>) MCF-7 cells were treated with siRNA targeting ANG or control for 48 hours. The mRNA levels of <i>ANG</i> and <i>ERRγ</i> were detected by RT-qPCR.</p
Information of ANG-binding fragments identified by ChIP-cloning.
No full text<p>Information of ANG-binding fragments identified by ChIP-cloning.</p
ANG interacts with ABSE fragment in HeLa and MCF-7 cells.
No full text<p>(<b>A</b>) Schematic illustration of ABSE localization in <i>ERRγ</i> gene. (<b>B</b>) HeLa cells were pretreated with or without neomycin for 1 hour and incubated with 1 ug/mL of ANG. Cells were then applied to ChIP experiments with IgG or ANG antibody and analyzed by qPCR. Data shown represents mean±s.d. of three independent experiments. (<b>C</b>) MCF-7 cells were pretreated with or without neomycin for 1 hour and incubated with 1 ug/mL of ANG. Cells were then stained with ANG polyclonal antibodies. Nuclei were stained with DAPI (blue). (<b>D</b>) MCF-7 cells were treated with or without neomycin followed by incubation with 1 ug/mL of ANG. Cells were then applied to ChIP experiments. Data shown represents mean±s.d. of three independent experiments.</p
ERRγ is involved in ANG-regulated cancer cell proliferation and cell cycle protein expression.
No full text<p>(<b>A</b>) MCF-7 cells transfected with siRNAs targeting ANG, ERRγ, or both were seeded at equal density. Cell numbers were counted at each time point as indicated. One-way ANOVA was used for statistical analysis of cell proliferation. (<b>B</b>) MCF-7 cells were transfected with siRNAs targeting ANG, ERRγ, or both and the expression levels of indicated genes were detected with RT-qPCR. (<b>C</b>) MCF-7 cells transfected with siRNAs targeting ANG, ERRγ, or both were applied to ChIP assays with antibodies against RNA Pol II or ERRγ. The antibody enriched DNA were analyzed by qPCR. Values were means±s.d. for triplicates.</p
Reinforcement of arteriolar myogenic activity by endogenous ANG II: susceptibility to dietary salt
No full text The purpose of this study was to determine whether endogenous ANG II augments arteriolar myogenic behavior in striated muscle. Because circulating ANG II is decreased during high salt intake, we also investigated whether dietary salt could alter any influence of ANG II on myogenic behavior. Normotensive rats fed low-salt (0.45%, LS) or high-salt (7%, HS) diets were enclosed in a ventilated box with the spinotrapezius muscle exteriorized for intravital microscopy. Dietary salt did not affect resting arteriolar diameters. Microvascular pressure elevation by box pressurization caused greater arteriolar constriction in LS rats (up to 12 μm) than in HS rats (up to 4 μm). The ANG II-receptor antagonists saralasin and losartan attenuated myogenic responsiveness in LS rats but not HS rats. The bradykinin-receptor antagonist HOE-140 had no effect on myogenic responsiveness in LS rats but augmented myogenic responsiveness in HS rats. HOE-140 with the angiotensin-converting enzyme inhibitor captopril attenuated myogenic responsiveness to a greater extent in LS rats than in HS rats. We conclude that endogenous ANG II normally reinforces arteriolar myogenic behavior in striated muscle and that attenuated myogenic behavior associated with high salt intake is due to decreased circulating ANG II and increased local kinin levels. </jats:p
ANG influences the histone modifications and Pol II occupation at ABSE region.
No full text<p>MCF-7 cells were transfected with siRNA targeting ANG or control siRNA for 72 hours. ChIP assays were performed with antibodies against acetyl-H4 (<b>A</b>), H3K4me2 (<b>B</b>), or RNA Pol II (<b>C</b>) and analyzed by qPCR. Values were means±s.d. for triplicates.</p
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