200,143 research outputs found
Ep. #141 - Lacy M. Johnson
No full textThis recording and transcript form part of a collection of podcasts conducted by the Cultures of Energy at Rice University. Cultures of Energy brings writers, artists and scholars together to talk, think and feel their way into the Anthropocene. We cover serious issues like climate change, species extinction and energy transition. But we also try to confront seemingly huge and insurmountable problems with insight, creativity and laughter.Dominic and Cymene report from Scotland where they have arrived for what looks to be an amazing Petrocultures 2018 event. Some talk of haggis and whiskey follows. But it’s also the anniversary of Hurricane Harvey back in Houston and to process how we feel about that (11:07) we invite our dear colleague, Lacy M. Johnson (http://www.lacymjohnson.com) into THE STUDIO to talk about where we find our heads at one year later. We talk about whether Harvey has shifted Houstonians’ willingness to accept climate change and Lacy talks about her own Harvey experience and how it motivated her to develop the Houston Flood Museum project, a virtual museum that launched this week (https://houstonfloodmuseum.org). Lacy explains why she thinks “discovery” might be a better way to think about life post-trauma rather than “recovery” and why it was compassion rather than strength that helped us through the disaster. We talk about her writing process and then turn from there to Lacy’s forthcoming book, The Reckonings (Simon & Schuster, http://www.simonandschuster.com/books/The-Reckonings/Lacy-M-Johnson/9781501159008), a marvelous collection of essays. We spend a little extra time on her harrowing account of the 43,000 tons of nuclear waste that were dumped in a North St. Louis landfill in the 1970s and the smoldering underground fire that is edging ever closer to the site. In closing, Lacy explains why we need to give ourselves permission to feel joy in an imperfect world because joy is a form that justice takes
[W. M. Lacy]
No full textW. M. Lacy served as Mayor of Palestine from 1873 until 1874 and then again from 1887 until 1889
Marriage record of Latum, Virgil A. and Wiggins, Lacy M.
No full textMarriage license for Virgil A. Latum and Lacy M. Wiggins. R.M. Evans was the officiant
Structural and functional implications of phase separation of membrane protein LacY in Escherichia coli
No full textThe dataset contains all optical and electron microscopy files in .czi, .lsm, .lif and .tif formats; raw .stk files of SMdM and PALM acquisitions; .csv output files from the TECAN plate reader used in the in the publication with the title: “Structural and functional implications of phase separation of membrane protein LacY in Escherichia coli “.
Files are organized based on the figure they were used in. Molecular dynamic simulations input files, trajectories and code for the analysis are available on Zenodo: 10.5281/zenodo.17335657.
Liquid-liquid phase-separation (LLPS) controls protein activity and dynamically organizes (macro)molecules in living systems without the need for membrane-bound compartments. Biomolecular condensates of water-soluble proteins have extensively been studied, but little is known about LLPS of membrane proteins. In this work we induce in vivo condensation of lactose permease (LacY), a widely-studied model monomeric inner membrane protein in Escherichia coli, and evaluate how it affects LacY function. We fused LacY with engineered, condensate-forming protein PopTag. We observe major changes in the localization and mobility of LacY-Pop. Molecular dynamics simulations show how the PopTag domain drives the condensate-like association dynamics of LacY-Pop through hydrophobic sticker interactions. LacY-Pop preserves native-level transport activity and outperforms the non-condensed LacY under mild hyperosmotic stress (osmotic upshift). In osmotically stressed cells, membrane-bound biomolecular condensates also reduce deformation of the cytoplasmic membrane. Perturbation experiments suggest that membrane curvature drives the accumulation of LacY-Pop at the poles of E. coli. Co-condensation of LacY and β-galactosidase LacZ slightly reduces their activity and results in remarkable cellular reorganization of the proteins. Our research shows the localization, dynamics, and function of phase-separated membrane proteins in bacteria and highlights the potential of LLPS for engineering complex metabolic networks in vivo
Relative domain folding and stability of a membrane transport protein
Get PDFThere is a limited understanding of the folding of multidomain membrane proteins. Lactose permease (LacY) of Escherichia coli is an archetypal member of the major facilitator superfamily of membrane transport proteins, which contain two domains of six transmembrane helices each. We exploit chemical denaturation to determine the unfolding free energy of LacY and employ Trp residues as site-specific thermodynamic probes. Single Trp LacY mutants are created with the individual Trps situated at mirror image positions on the two LacY domains. The changes in Trp fluorescence induced by urea denaturation are used to construct denaturation curves from which unfolding free energies can be determined. The majority of the single Trp tracers report the same stability and an unfolding free energy of approximately +2kcal mol(-1). There is one exception; the fluorescence of W33 at the cytoplasmic end of helix I on the N domain is unaffected by urea. In contrast, the equivalent position on the first helix, VII, of the C-terminal domain exhibits wild-type stability, with the single Trp tracer at position 243 on helix VII reporting an unfolding free energy of +2kcal mol(-1). This indicates that the region of the N domain of LacY at position 33 on helix I has enhanced stability to urea, when compared the corresponding location at the start of the C domain. We also find evidence for a potential network of stabilising interactions across the domain interface, which reduces accessibility to the hydrophilic substrate binding pocket between the two domains
Superstition Rapid, Colorado [10]
No full textColor slide photo by Joe M. Lacy of a view of Superstition Rapid on the Colorado River near Glenwood Springs, Colorado, in July of 1959
Superstition Rapid, Colorado [01]
No full textColor slide photo by Joe M. Lacy of boaters running Superstition Rapid on the Colorado River during a SOCOTWA outing in southern Utah in July of 1959
Superstition Rapid, Colorado [06]
No full textColor slide photo by Joe M. Lacy of a boat navigating Superstition Rapid on the Colorado River near Glenwood Springs, Colorado, in July of 1959
Superstition Rapid, Colorado [08]
No full textColor slide photo by Joe M. Lacy of people at Superstition Rapid on the Colorado River near Glenwood Springs, Colorado, in July of 1959
Superstition Rapid, Colorado [07]
No full textColor slide photo by Joe M. Lacy of a boat navigating Superstition Rapid on the Colorado River near Glenwood Springs, Colorado, in July of 1959
- …
