1,721,004 research outputs found

    The IVC breeding program: development of new seedless table grapevines for a sustainable viticulture

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    During 2015-2020, the Italian Variety Club, a consortium of growers interested in more sustainable viticulture, carried out an extensive breeding program in Puglia to produce seedless grapevines resistant to powdery (PM) and downy mildew (DM), the two most damaging grapevine diseases, and well adapted to the southern Italian climate. One hundred-nine table grapevine seedless cultivars were crossed with elite cultivars cultivated in the area or with resistant cultivars. The obtained 118.271 seedlings were subjected to marker-assisted selection for seedlessness with the marker VvAGL11, and for resistance to downy mildew and powdery mildew with 8 specific markers. The program allowed to obtain 7.696 putative seedless hybrid genotypes, corresponding to 49% of the total progeny, that were submitted to agronomic evaluation in the field, which allowed to select 10 promising cultivars which were entered in the National Register of Vine Varieties (RNVV). As for resistance, 672 seedless genotypes were found resistant to PM and DM, and 81 showed pyramidization of resistances. The work discusses the main parameters related to pollination, vernalisation, germination and plant recovery efficiency, the impact of parental combinations and other variables involved in defining the efficiency of such types of selection programmes, helping to promote a more sustainable and environmentally friendly viticulture in Puglia

    Genetic variability and population structureof Grapevine virus A coat protein gene from naturallyinfected Italian vines

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    Grapevine virus A (GVA) is considered one of the viruses associated with rugose wood (RW), one of the most economically important diseases of grapevine. Thirty-seven GVA isolates collected from grapevine cultivars from Marche (central-eastern Italy), Apulia and Campania (southern Italy), were subjected to molecular characterization. The genetic and population diversity was studied in the coat protein (CP) gene by RT-PCR-RFLP analysis with three restriction enzymes (MseI, AluI, and AciI), and nucleotide sequencing. A new primer pair (CP1F/R) allowing amplification of the whole CP gene (621 bp) was developed. RFLP with AciI yielded the highest number of variants in GVA isolates, showing seven different ‘simple’ profiles (A, B, C, D, E, F, and G). ‘Complex’ profiles were also found, and the most common variant combination was A+B in 39% of isolates. The analysis of GVA sequences confirmed the presence of plants infected with more than one GVA variant and suggested that RT-PCR-RFLP is suitable for evaluating population diversity of GVA enabling a screening of different haplotypes. The distribution of RFLP profiles and the phylogenetic analysis were not correlated with the location of infected plants, showing the presence of a GVA population with genetic diversity in the average with those of RNA viruses

    Correlation between grape and wine quality, landscape diversity, on-field biodiversity, in doc Gioia del Colle, Italy

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    Analysis of aerial photos by using GIS tools and on-field surveys of flora are used to characterize territories from an agro-ecological point of view and to assess the level of diversity of given agro-ecosystems. More and more correlations between landscape characteristics, sustainability and quality of agriculture production were speculated. In last three years a study was carried out in the area of DOC “Gioia del Colle” in Apulia, South Italy, in order to characterize and investigate different vineyards and sites and find out possible interactions and correlations between the landscape diversity, the biodiversity of fields and the quality of grapes and wines. In order to investigate such aspects and achieve a better knowledge of such correlations, 13 vineyards were continuously monitored for qualitative aspects related to grapevine phenology, growing and cultivation as well as for investigating quality of flora and biodiversity of spontaneous plants. A landscape analysis was carried out identifying a buffer area of a radium of 5 km around each of the vineyards and by carrying on the calculation of a set of indicators able to quantify landscape structure, composition and level of connection on the basis of the different shape, areas and cover of the patches. Statistical correlations between values of the landscape indicators, biodiversity of spontaneous plants of the different representative vineyards and the specific characteristics of the grapes were analyzed. A preliminary better knowledge of the interactions between such elements of the landscape, the sustainability of grapevine cultivation and the quality of the grapes was achieve
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