1,720,995 research outputs found
METHOD FOR CELL REPROGRAMMING AND DIFFERENTIATION BY MICROFLUIDIC TECHNOLOGY
No full textA method for reprogramming differentiated cells and for then converting the reprogrammed cells into a differentiated phenotype of interest by means of microfluidic technology is described, together with a related kit for cell reprogramming
Microfluidics for secretome analysis under enhanced endogenous signaling
No full textCell secretome, the complex set of proteins that are secreted by the cells, is a fundamental mechanism of
cell-cell communication both in vitro and in vivo. In vivo, the analysis of proteins secreted into body fluids
can bring to the identification of biomarkers for important physiopathological conditions. However, due
to the complexity of the protein content of body fluids, a better understanding of the secreted proteins by
different cell types is highly desirable and can be performed in vitro for dissection. To this aim, microfluidic
culture systems could be particularly relevant because of the accumulation of extrinsic endogenous
signals at microliter scale, which better preserves the self-regulation occurring in the small
interstitial spaces in vivo. In this work, we perform a quantitative study to compare the secretome in
microfluidics and in a standard well plate. Human foreskin fibroblasts are used as a case study. This work
also represents an important technological advance in terms of feasibility of high-throughput quantitative
protein analyses in microfluidics
Mechanical mixing device for cell culture multiwells
No full textDispositivo per la miscelazione meccanica di colture
cellulari in sospensione contenute in una piastra di
coltura, comprendente: - un coperchio 10 destinato ad
essere amovibilmente associato ad una piastra di
coltura P per schermare il pozzetto o i pozzetti W di
quest’ultima limitando l’ingresso di solidi sospesi
nell’aria ambiente, e al contempo permettendo
l’ingresso di gas e umidità esterni; - almeno una
girante di miscelazione 20 per ciascun pozzetto W della
piastra di coltura P; - mezzi motori 30 collegabili
cinematicamente a ciascuna girante 20.
La girante 20 è rotazionalmente associata al coperchio
10 in corrispondenza della faccia di quest’ultimo
destinata ad essere rivolta verso detta piastra di
coltura P. Almeno il coperchio 10 e le giranti 20 sono
realizzate con materiali che possono essere sottoposti
a trattamenti di sterilizzazione e sono in grado di
resistere alle condizioni di umidità, temperatura e pH
che si creano in un incubatore biologic
Microfluidic technology enhances the potential of human pluripotent stem cells
No full textSince the discovery of human somatic cell reprogramming, human induced pluripotent stem cells (hiPSC)
have been increasingly recognized as the landmark for development of organs-on-chip. hiPSCs show a
remarkable plasticity that is related to their ability to promptly respond to the surrounding environment.
In vitro, the soluble culture microenvironment, with its critical balance between exogenous and cellsecreted
factors, plays a great role in inducing hiPSC response, for both preserving pluripotency and
controlling differentiation stages. Exploring the complexity of hiPSC microenvironment requires new
experimental tools, as a tight control is limited within conventional culture dishes. Microfluidic technology
is particularly attractive in hiPSC research because of its ability to mimic specific environmental
cues by accurate control of soluble factors with high spatiotemporal resolution and in a high-throughput
fashion. In this review, we highlight recent progress in hiPSC research enabled by microfluidic technology
as well as new emerging scenarios
Modelling of detailed insulin receptor kinetics affects sensitivity and noise in the downstream signalling pathway
No full textInsulin resistance is a primary defect underlying the development of type II diabetes.
In healthy conditions, insulin stimulates glucose uptake from the blood stream, but in diseased
conditions the normal metabolic response is impaired. Identifying specific drug targets to restore
insulin sensitivity at the cellular level and developing an effective treatment strategy require
insight into both the biochemical mechanisms involved and the whole signalling network response
to external cues. This study focuses on the consequences of integrating a detailed biochemical
description of the insulin receptor trafficking compartment within a phenomenological model of
the downstream signalling pathway. While the description of the experimental data is preserved
by an iterative procedure of parameter fitting, the dynamic response of the network is highly
modified, as shown by analyzing the complementary information derived from studying both
connection sensitivities and node noise in the network. This is crucial considering the importance
of network dynamics for identifying effective drug targets
Determination of glucose metabolic fluxes in live myoblasts by microfluidic nanosensing and data analysis
No full textEditorial: New trends in biomimetic tissue and organ modelling
No full textAn innovative journal that explores technologies which can maintain healthy lives and contribute to the global bioeconomy by addressing key medical and healthcare needs
Modeling of detailed insulin receptor kinetics affects downstream signaling sensitivity in adipocytes
No full textEditorial: 3D-engineered organoids for modelling tissue development and precision medicine
No full textA multidisciplinary journal that explores the opportunities for chemical engineering to address some of the key challenges we face - energy, health, food, manufacturing and the environment
Microtechnology for stem cell culture
No full textAdvances in stem cell research in recent decades have been aided by progress in the development of novel technologies aimed at biological systems. At the same time mimicking stem cell niches in vitro has become crucial for both basic stem cell research and the development of innovative therapies based on stem cells. Innovative microscale technologies can contribute to our quantitative understanding of how phenomena at the microscale can determine stem cell behavior based on our increasing ability to control culture conditions and the throughput of data while reducing times and costs. In particular, microtechnologies must be designed and developed to capture the complexity of cell–substrate, cell–cell, and cell–soluble environment interactions considering the characteristic time and length scales of biological phenomena. While acknowledging the advantages of applying these technologies to stem cell culture, this chapter focuses on issues related to the control and mimicking of microenvironmental cues of the stem cell niche, such as substrate properties, cell topology, the soluble environment, and the electrophysiology
- …
