1,720,972 research outputs found
Studio dell’attività citotossica monocitaria nel trattamento con alpha Interferon di pazienti HBV e/o HDV positivi in attesa di trapianto d’organo
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Analysis of methods commonly used for glycopeptide and oxazolidinone susceptibility testing in Enterococcus faecium isolates
No full textThe susceptibility to teicoplanin, vancomycin and linezolid of 30 clinical isolates of Enterococcus faecium was tested by Vitek 2, Phoenix, Etest, broth microdilution and disc diffusion tests. The vanA and vanB resistance genes and the 23S rRNA gene G2576T mutation were detected by PCR and PCR-RFLP, respectively. Resistance rates to teicoplanin ranged from 3% for Vitek 2 to 57.6% for the Phoenix test, and those to vancomycin ranged from 56.7% for Vitek 2 to 86.7% for the Phoenix test. Only two out of 25 strains carrying the vanA gene were univocally recognized as the VanA phenotype. The only strain with the G2576T mutation did not carry the vanA gene and showed resistance to linezolid by the disc diffusion, Vitek 2 and broth dilution methods (MIC>8 microg ml(-1)), but was susceptible when tested with the Phoenix test and Etest (MIC<or=4 microg ml(-1)). Therefore, the resistance to glycopeptides and linezolid was not univocally detected by the susceptibility testing methods used in this study
REACTIVITY AND PROTECTIVE CAPACITY OF A POLYCLONAL ANTISERUM DERIVED FROM MICE IMMUNIZED WITH ANTIBIOTIC EXPOSED ESCHERICHIA-COLI
No full textThe murine immune response to Escherichia coli O6:K- alone or pre-exposed to 0.1 x MIC of aztreonam was investigated. Relative to mice immunized with untreated bacteria, mice immunized with antibiotic-treated microorganisms presented a significantly enhanced protection towards a challenge of 100 x LD50 of viable E. coli O6:K-. Previous injection of 0.1 mL of serum drawn from mice immunized with treated and untreated bacteria protected non-immunized mice towards a challenge of 10 x LD50 of viable E. coli O6:K-. Serum from mice immunized with treated bacteria also protected non-immunized mice towards a lethal challenge of E. coli O111. The antiserum contained high titre of IgG antibodies that cross-reacted with lipopolysaccharide isolated from smooth and rough Gram-negative bacteria. Immunoblotting showed additional bands of reactivity to the untreated E. coli O6:K-. Immunization with antibiotic-treated bacteria led to the production of type specific and cross reactive antibodies that protected animals against viable homologous and heterologous lethal challenges
Cross protection and reactivity of a polyclonal antiserum derived from mice immunized with antibiotic treated bacteria
No full textPolymerase Chain Reaction, with sequencing, as a diagnostic tool in culture-negative bacterial meningitis
No full textImmunizzazione di topi con E. coli trattato con dosi sub-MIC di antibiotico monobattamico
No full textImmunization of mice with antibiotic treated E.coli enhances antibody production and protects against lethal challenge
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