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IDENTIFICATION OF CLAVIBACTER-MICHIGANESIS SUBSP SEPEDONICUS USING THE POLYMERASE CHAIN-REACTION
No full textFrom the sequence of a subcloned DNA fragment of a highly conserved plasmid of Clavibacter michiganensis subsp. sepedonicus a pair of oligonucleotides were devised for use as polymerase chain reaction primers. The primer sequences do not show significant homology with any other sequence deposited in public databases. Polymerase chain reactions carried out using this primer pair and untreated cells of all strains of C. michiganensis sepedonicus tested resulted in the amplification of a DNA fragment of about 670 base pairs. No amplification was observed when bacteria belonging to other species were submitted to polymerase chain reaction under the same conditions. The detection limit of the assay was 4 X 10(3) bacteria
RAPID PREPARATION OF DNA FROM PHYTOPATHOGENIC MYCOPLASMA-LIKE ORGANISMS FOR POLYMERASE CHAIN-REACTION ANALYSIS
No full textA simple procedure for the processing of MLO (mycoplasma-like organisms)-infected plant samples for PCR analysis is described. It is based on differential centrifugation and removal of enzyme inhibiting agents with the aid of a resin. Results of the amplifications are comparable with those achieved with more complex DNA extraction procedures. The method does not require any extraction with organic solvents or alcoholic precipitation of nucleic acids
Production of oligonucleotide probes for mycoplasma-like organisms using the polymerase chain reaction
No full textGrowth kinetics of Botrytis cinerea on organic acids and sugars in relation to colonisation of grape berries
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Sequence analysis of domains III and IV of the 23S rRNA gene of verticillate streptomycetes
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Mitochondrial plasmids of the pCp family are spread worldwide in Cryphonectria parasitica populations
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