1,721,259 research outputs found
Anthraquinones: analytical techniques as a novel tool to investigate on the triggering of biological targets
No full textApproximately, 63% of marketed drugs derive from natural products or their semi synthetic derivatives. Compounds from living organisms often exert a biological activity, triggering several targets, which may be useful for the improvement of novel pharmaceuticals. These natural products can be extracted from plants, marine organisms, or microorganism fermentation broths. In the vast array of bioactive secondary metabolites known up to now, anthraquinones are among the most investigated natural products, in particular for what concerns their mechanism of action. This review focuses on the analytical aspects of anthraquinones, from their separation to recent and new highthroughputs techniques for the simultaneous determination of these analytes in biological matrices that can greatly contribute to sharply depict the targets of these secondary metabolites as well as on an updated survey of their biological activities
Campionamento e dosaggio di analiti d’interesse farmaceutico da gocce di sangue secco mediante LC-MS/MS
No full textAdvanced Capillary Electrophoresis Techniques in the Analytical Quantification of Drugs, Metabolites and Biomarkers in Biological Samples
No full textCapillary electrophoresis offers several advantages in terms of higher chromatographic resolution, faster analysis, smaller sample volume required, lower cost due to very cheap capillaries (if compared to expensive
HPLC or GC columns) and low organic solvent consumption. It is a powerful assay, in particular, applied to biological samples analyses (blood, urine, cerebrospinal fluid) and especially where a small amount of sample is available, such as pharmaceutical and biomedical analyses. For these reasons this technique is suitable to quantify drugs, metabolites and biomarker in biological fluid, where LOD and/or LOQ values at lower g/L range are
required. In addition, under special condition, the technique can be applied also in presence of other sample constituents like protein or other macromolecules. The only limit of this method is represented by smaller sample
volume that implies a relatively poor LODs values mainly caused by the small injection volume and when UV detection is used, the short optical path length attributable to the low inner diameter of the capillary. This review intends to explore the new frontiers of capillary electrophoresis techniques in terms of sensitivity improvement, bioanalytical (and pharmacological) applications, instrument configurations especially underlining its versatility
and potentiality
Ultra-trace heavy metal determination in sea food by differential pulse catalytic voltammetry: application to fishes and bivalve molluscs
No full textThe paper proposes an analytical procedure regarding the voltammetric determination of chromium(VI), copper(II), lead(II), cadmium(II), zinc(II) and mercury(II) by differential pulse catalytic voltammetry (DPCV) in matrices involved in the food chain, but also employed as possible biological monitors for trace metal. The digestion of each matrix was carried out using a concentrated HCl-HNO3-H2SO4 acidic attack mixture. 0.23 mol L-1 dibasic ammonium tartrate buffer pH 4.9 + 5.9x10-2 mol L-1 NaBrO3 was employed as the supporting electrolyte. The voltammetric measurements were carried out using, as working electrodes, a stationary hanging mercury drop electrode (HMDE) [simultaneous determination of Cr(VI), Cu(II), Pb(II), Cd(II) and Zn(II)] and a gold electrode (GE) [determination of Hg(II)], a platinum electrode and Ag⎪AgCl⎪KClsat electrode as auxiliary and reference electrodes, respectively. The analytical procedure has been verified on the standard reference materials Mussel Tissue BCR-CRM 278 and Cod Muscle BCRCRM 422. For all the elements in the certified matrices, the precision as repeatability, expressed as relative standard deviation (sr) was lower than 6 %, while the trueness, expressed as relative error (e) was of the order of 5-6 %. Once set up on the standard reference materials, the analytical procedure was transferred and applied to fishes and molluscs sampled in the Po river mouth area
Simultaneous determination of meropenem, ciprofloxacin, and ofloxacin in human plasma using RP-HPLC-DAD method development, validation and optimization of various experimental parameters
No full textcomunicazione poste
Toxic Metals in Herbal Medicines. A Review
No full textThe present review proposes an exhaustive focus on what are the metals of interest, and what is the state of the art about analytical methodologies suitable to detect these toxic metals in herbal medicines. This review would also be a stimulus to solicit International Organizations to fill the gap of the lack of strict and comprehensive laws regulating the maximum allowable concentrations for an increasing number of contaminants in these matrices, especially considering their enormous consumption.
Herbal medicines are more and more worldwide used. This fact certainly presents serious problems for the potential human health risks. This is due to the fact that the laws in force generally do not provide for strict quality controls of herbal medicines to certify the concentration of compounds and elements that may be hazardous for human health, and sometimes very severe or even lethal. Heavy metals have a decidedly substantial part of the contaminants in herbal medicines
Ultra-trace heavy metal determination in sea food by differential pulse catalytic voltammetry: application to fishes and bivalve molluscs
No full textThe paper proposes an analytical procedure regarding the voltammetric determination of chromium(VI), copper(II), lead(II), cadmium(II), zinc(II) and mercury(II) by differential pulse catalytic voltammetry (DPCV) in matrices involved in the food chain, but also employed as possible biological monitors for trace metal.
The digestion of each matrix was carried out using a concentrated HCl-HNO3-H2SO4 acidic attack mixture.
0.23 mol L-1 dibasic ammonium tartrate buffer pH 4.9 + 5.9x10-2 mol L-1 NaBrO3 was employed as the supporting electrolyte. The voltammetric measurements were carried out using, as working electrodes, a stationary hanging mercury drop electrode (HMDE) [simultaneous determination of Cr(VI), Cu(II), Pb(II), Cd(II) and Zn(II)] and a gold electrode (GE) [determination of Hg(II)], a platinum electrode and Ag⎪AgCl⎪KClsat electrode as auxiliary and reference electrodes, respectively. The analytical procedure has been verified on the standard reference materials Mussel Tissue BCR-CRM 278 and Cod Muscle BCRCRM
422. For all the elements in the certified matrices, the precision as repeatability, expressed as relative standard deviation (sr) was lower than 6 %, while the trueness, expressed as relative error (e) was of the order of 5-6 %. Once set up on the standard reference materials, the analytical procedure was transferred and applied to fishes and molluscs sampled in the Po river mouth area
A validated HPLC-UV/Vis methodology for the quantification of oxyprenylated cinnamic acids in Citrus spp.
No full text- …
