1,825,543 research outputs found
LL-18 binds directly to virus particles.
No full text(A) 1H NMR data showing the chemical shifts. Spectra of LL-37 (left panel) or LL-18 (right panel) peptide in the absence or presence of EV71 virus (green line) or CVB5 virus (red line). (B-C) 293T cells cotransfected with GFP or GFP-tagged LL-18 (B) or LL-37 (C) and HA-tagged VP1, VP2, or VP3 plasmids were immunoprecipitated with anti-HA antibody, and interactions between LL-18 and viral structural proteins were detected by anti-GFP antibody.</p
Enantioselective Synthesis of (−)-LL-C10037α from Benzoquinone
No full textThe enantioselective total synthesis of the Streptomyces metabolite (−)-LL-C10037α has been accomplished in 10 steps and 20% overall yield.
An early chiral intermediate was resolved with Candida rugosa lipase to provide (+)-5 with an enantiomeric excess ≥98%. The synthesis is
notable in that no protecting groups are required and that all carbons in the core structure of LL-C10037α are derived from the readily
available p-benzoquinone
LL-18 interferes with virus-SCARB2 interaction.
No full text(A) Molecular docking model of LL-18 with EV71 virion. EV71 capsid proteins VP1 (green), VP2 (pink), and VP3(blue), all interact with LL-18 displayed in red sticks (left panel). The amino acid residues of capsid protein interacting with both LL-18 and SCARB2 are indicated (right panel). (B) 293T cells expressing SCARB2-Flag were lysed and cell lysates were incubated with EV71 virus in the absence or presence of the indicated amount of LL-18. Cell lysates were then immunoprecipitated with anti-Flag antibody and the precipitated virus was determined with anti-VP1 antibody. (C) 293T cells expressing Annexin 2-Flag were lysed and cell lysates were incubated with EV71 virus in the absence or presence of LL-18. Cell lysates were then immunoprecipitated with anti-Flag antibody and the precipitated virus was determined with anti-VP1 antibody.</p
LL-18 inhibits mEV71 infection <i>in vivo</i>.
No full text(A) mEV71 was pre-treated with PBS or LL-18 and used to infect 6-day old ICR mice. The survival rate of mice infected with the mEV71 virus with or without LL-18 was recorded. (B) Mice infected with the mEV71 virus with or without LL-18 pre-treatment were sacrificed 1 day post infection (d.p.i.) and viral titers from brain and muscle tissues were determined. ***, P<0.001. (C) H&E staining of muscle tissue from mice infected with the mEV71 virus with or without LL-18. Blue arrows indicate infiltrated neutrophils. Bar, 20μm.</p
LL-18 binds directly to virus particles.
No full text(A) RD cells were pre-incubated with indicated amounts of LL-18, FF-18, DL-37, or LL-37 for 2 hrs before they were washed extensively to remove unbound peptides. Cells were then infected with the EV71 virus (MOI = 1) and cell viability was determined 24 h.p.i. Values were normalized to uninfected RD cells. ***, P1H NMR spectra of FF-18 peptide in the absence or presence of EV71 virus (green line) or CVB5 virus (red line). (TIF)</p
mit-ll-responsible-ai/hydra-zen: Release v0.7.0
No full textSee changelog for descriptive summary.
What's Changed
Improve type annotations by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/244
reorg: move make_config implementation to its own file by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/245
Internal code reorg by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/246
Fix overloads by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/247
Add missing overloads for make_custom_builds_fn by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/248
Remove unnecessary type: ignore comment by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/249
Update partial stub by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/251
Update docs dependecies by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/252
Extend the functionality of just to work with all Hydra/hydra-zen supported types by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/250
Update README.md by @rlbellaire in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/254
Update README.md by @rlbellaire in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/255
Fix failing pyright 1.1.236 by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/258
to_yaml and save_yaml now reflect hydra-zen's extended type support by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/259
fix instantiate overloads for Any by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/260
Enable nested container types for omegaconf 2.2.0 by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/261
Enable native support for bytes for omegaconf 2.2.0 by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/262
Deprecates builds_bases via make_custom_builds by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/263
Adds hydra_defaults to builds and make_config by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/264
Fix overly-permissive annotation: nested tuples by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/267
Fix builds() derived via inheritance by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/266
speedup common cases of sanitized_default_value by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/268
remove redundant tests by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/270
Update CI by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/271
Updates to launch by @jgbos in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/272
Adds version_base to launch by @rsokl in https://github.com/mit-ll-responsible-ai/hydra-zen/pull/273
Full Changelog: https://github.com/mit-ll-responsible-ai/hydra-zen/compare/v0.6.0...v0.7.
Sequence and the expression construct of SP-LL-37.
No full text(A) Nucleotide sequence of SP-LL-37 containing the coding region and stop codon as well as the signal peptide (underlined). (B) Schematic diagram of the expression construct pSP1::SP-LL-37. Detection of Nos gene copy numbers in the T0 generation of pPZP::SP-LL-37 plants. (C) PCR analysis of SP-LL-37 transgenic rice. Lanes: M; molecular marker, P; pPZP::SP-LL-37 vector, NT; non-transformed (NT) control, 1~25; independent T0 transgenic lines. (D) TaqMan PCR analysis for copy number assays using TaqMan probe for single copy selection in T0 transgenic Rice. +; single copy, -; multi copy, T2-homozygous and T2-heterozygous; single copy control, NT; negative control, WT; wild type control.</p
Enantioselective Synthesis of (−)-LL-C10037α from Benzoquinone
No full textThe enantioselective total synthesis of the Streptomyces metabolite (−)-LL-C10037α has been accomplished in 10 steps and 20% overall yield.
An early chiral intermediate was resolved with Candida rugosa lipase to provide (+)-5 with an enantiomeric excess ≥98%. The synthesis is
notable in that no protecting groups are required and that all carbons in the core structure of LL-C10037α are derived from the readily
available p-benzoquinone
LL-37 signaling increases CovS phosphatase activity.
No full text(A) Representative Western blot (top) and bar representation (bottom) depicting CovR~P levels and (B) hasA transcript levels (means ± standard deviations, n = 4) of indicated GAS strains grown to mid-exponential phase in THY or THY supplemented with 100nM LL-37. *** denotes significant difference (PhasA transcript levels of indicated strain grown in THY and THY + LL-37 as determined using Student’s t-test using Bonferonni’s correction for multiple comparisons.</p
Identification of pPZP::SP-LL-37 insertion transgenic plants.
No full textThe genomic structures of insertion alleles were determined by FST analysis in which boxes, bold lines, and triangles indicate exons, intron, and pPZP::SP-LL-37, respectively. The arrow and arrowhead indicate gene specific primer pairs from genomic DNA. Genomic DNA was isolated from the leaves of pPZP::SP-LL-37 regenerated plants for PCR analysis. WT: wild type, T: T0 plant with single T-DNA insert for pPZP::SP-LL-37 insertion line.</p
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