171 research outputs found
Supplemental_material_for_DNA_Switch_Toehold_Mediated_DNA_Isothermal_Amplification_for_Dengue_Serotyping_by_Chan,_et_al – Supplemental material for DNA Switch: Toehold-Mediated DNA Isothermal Amplification for Dengue Serotyping
No full textSupplemental material, Supplemental_material_for_DNA_Switch_Toehold_Mediated_DNA_Isothermal_Amplification_for_Dengue_Serotyping_by_Chan,_et_al for DNA Switch: Toehold-Mediated DNA Isothermal Amplification for Dengue Serotyping by Soo Khim Chan, Akinori Kuzuya, Yee Siew Choong and Theam Soon Lim in SLAS Discovery</p
Supplementary material - Supplemental material for Chemical Constituents From <i>Endiandra kingiana</i> (Lauraceae) as Potential Inhibitors for Dengue Type 2 NS2B/NS3 Serine Protease and its Molecular Docking
No full textSupplemental material, Supplementary material, for Chemical Constituents From Endiandra kingiana (Lauraceae) as Potential Inhibitors for Dengue Type 2 NS2B/NS3 Serine Protease and its Molecular Docking by Syazreen N. Sulaiman, Maywan Hariono, Haslinda Mohd Salleh, Soon-Lim Chong, Liew Sook Yee, Azeana Zahari, Habibah A. Wahab, Severine Derbré and Khalijah Awang in Natural Product Communications</p
Naïve antibody library derived monoclonal antibody against VP35 of Ebola virus
No full textEbola virus is notorious for causing severe and even deadly haemorrhagic fever in infected humans and non-human primates. The high fatality rate of Ebola virus disease (EVD) has highlighted the need for effective diagnosis and treatment. Two monoclonal antibodies (mAbs) have been approved by USFDA for treatment of EVD. Virus surface glycoprotein is the common target for diagnostic and therapy including vaccines. Even so, VP35, a viral RNA polymerase cofactor and interferon inhibitor could be a potential target to curb EVD. The present work describes the isolation of three mAb clones from a phage-displayed human naïve scFv library against recombinant VP35. The clones showed binding against rVP35 in vitro and inhibition of VP35 in luciferase reporter gene assay. Structural modelling analysis was also carried out to identify the binding interactions involved in the antibody-antigen interaction model. This allows some insight into the "fitness" of the binding pocket between the paratope and target epitope which would be useful for the design of new mAbs through in silico means in the future. In conclusion, the information obtained from the 3 isolated mAbs could be potentially useful in the quest to improve VP35 targeting for therapeutic development in the future
DESA : a design method that reduces system complexity
No full textThis research deals with a fonnal design method called "Design Equations for
Systems Analysis", or DESA. This method reduces system complexity, and has been
demonstrated to effectively reduce the complexity of human-machine systems. It also
increases the usability of products. The author summarized two other design methods
that reduce system complexity - Axiomatic Design (AD) and Design Structure Matrix
(DSM) - and explained their differences from DESA. A case study was then
presented to demonstrate the ability ofAD and DSM to complement each other. Since
DESA builds on AD, it can complement DSM similarly. The author revised the
framework that DESA utilizes. A tenninology was established to define the technical
tenns in DESA. DESA was then employed to design an inspection method that
evaluates usability. It was also employed to reduce the complexity of an objectoriented
software system. These two applications of DESA are presented as case
studies. These two case studies respectively demonstrate that DESA designs for
usability and effectively reduces the complexity of object-oriented software systems.MASTER OF ENGINEERING (MAE
Development of a pico-satellite : sun sensors for attitude determination
No full textFollowing the completion of the micro-satellite XSAT program, and currently waiting for the launch, NTU has started another program to develop a pico- and nano-satellite pair VELOX-I. This program includes many FYP projects working on the various sub-systems of a satellite.
For a satellite to determine and control its location and orientation in space, the Attitude Determination and Control System (ADCS) is an essential sub-system for the task. This project focuses on the sun sensor in the ADCS which determines the sun direction with respect to the vertical axis of the satellite. The sun direction will allow the satellite to orientate itself perpendicular to the sun direction for maximum solar power collection and also to shelter sensitive equipments away from the strong solar radiation.
In this project, hardware prototype has been designed and implemented on PCB. It has been tested using a sun simulator to simulate real parallel sunrays and a 2-axis turn motor to rotate the sensor. The hardware will provide four voltage analog outputs dependent on the sun spot position.
The voltage outputs is first processed by the microcontroller. Subsequently, the digital data is transferred to PC for noise removal using Kalman filter, computation of sunray angle and error calibration based on the analysis of errors caused by mechanical and electrical design.
The accuracy for the sensor is -1.3º to 0.7º for a field-of-view of -30º to 30º, and ±0.7º for a field of view of -25º to 25º. This performance is comparable to commercial products that have accuracies less than 1º. The prototype has also been tested in outdoors under the real sun and proven to function satisfactorily.Bachelor of Engineerin
Differing roles of axiomatic design and design structure matrix in reducing system complexity
No full textNumerical simulation studies of the effect of membrane permeance on mass transfer enhancement driven by transient slip velocity
No full textOne of the most noteworthy achievements in reverse osmosis (RO) efficiency is the improvement in membrane permeance. Although current membranes offer higher permeance (and flux) than older RO membranes, increases in permeate flux are limited by concentration polarisation (CP) and fouling. Therefore, innovation is needed to reduce CP to further increase permeate flux. An unsteady forced slip velocity can disrupt the boundary layer, thus reducing CP. This paper uses Computational Fluid Dynamics (CFD) to analyse the effect of membrane permeance on the resonant frequency for an unsteady forced slip velocity, as well as the resulting mass transfer enhancement. The results show that the resonant frequency of the unsteady forced slip velocity is not affected by the membrane permeance. Although the results show a peak in the mass transfer enhancement factor for permeance values in the range typically used for brackish water, In addition, this thesis found that that at any membrane permeance, slip velocity can increase the maximum shear stress
Analysis and study of sea to land links
No full textBig stable and sailing ships in the open sea might be a blockage for line of sight (LOS)
propagation for wireless link between the shore and sea. These blockages are classified as
non line of sight (NLOS) propagation, which will somehow interrupt the sea to land
transmission.
This report will study the setup for channel characterization using spread spectrum channel
surrounding with Pseudo-random Noise (PN) Sequence. The focus will be on the LOS and
NLOS propagation to identify the minimum stand-off distance from the ship.Bachelor of Engineerin
Study of chromosomal aberrations in Myelodysplastic syndrome in an Asian population
No full textMyelodysplastic syndromes (MDS) are bone marrow disorders diagnosed predominantly in the elderly. Past studies on MDS patients in Asian and Western countries have exhibited differences in the characteristics of patients between these two populations. In this study, the types of chromosomal aberrations (CA) present in MDS patients in Singapore were investigated and subsequently compared to previous findings of Asian and Western patients. The prognoses of these patients were also determined to predict their survival and rate of progression to acute myeloid leukaemia. A retrospective analysis was carried out on 340 patients diagnosed with MDS by conventional cytogenetics (CC) and fluorescence in situ hybridization (FISH) between 2010 and 2014. The majority of these patients were found to have complex karyotypes of more than three aberrations, which is a common occurrence in Asian countries but not in the Western population. However, the median age of the patients were found to be closer to that of the Western population instead. These suggest that genetic and environmental factors may play a role in causing such differences between Asian and Western populations. Additionally, locus-specific FISH uncovered abnormalities missed by CC, suggesting that it is a good adjunct diagnostic test to CC in identifying subtle CA.Bachelor of Science in Biological Science
- …
