1,721,011 research outputs found

    Una microtecnica per allestire campioni da singole larve di pesci teleostei per la diagnosi della ploidia tramite citofluorimetria a flusso.

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    Una microtecnica per allestire campioni da singole larve di pesci teleostei per la diagnosi della ploidia tramite citofluorimetria a flusso

    A method for flow cytometric assessment of ploidy in single early hatched larvae of marine teleost fish.

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    The paper describes a method set up for the flow cytometric assessment of ploidy in single early hatched larvae of European sea bass (Dicentrarchus labrax). It represents an useful tool in chromosome set manipulation in fishes

    Riproduzione e manipolazione gametica di Umbrina cirrosa (L.) per la diversificazione dell’acquacoltura nazionale.

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    Relazione tecnica dell'attività scientifica IV° Piano Triennale per la Pesca e l'Acquacoltura. Ministero delle Politiche Agricole e Forestali

    Characterization of mitotic chromosomes of four species of the genus Diplodus: Karyotypes and chromosomal nucleolar organizer region phenotypes

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    Karyotypes have been described in four Mediterranean species of the genus Diplodus (Teleostei, Sparidae), D. vulgaris, D. puntazzo, D. sargus and D. annularis. Chromosomes were mainly acrocentric in all but D. vulgaris, where certain chromosomes pairs were subtelocentric. A remarkable intraspecific heteromorphism in the number of NOR-bearing chromosomes along with a substantial interspecific variability in position of chromosomal Ag signals have been encountered. The presumed origin of multiple NOR-bearing chromosomes in Diplodus species and variation of the NOR location is discussed

    Cytogenetic characterization of the moray eel Gymnothorax tile and chromosomal banding comparison in Muraenidae (Anguilliformes)

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    The karyotype of the teleostean Gymnothorax tile was analysed by replication banding in order to clearly identify the homologous chromosomes. The RBG pattern was then compared to those of the other Muraenidae studied (G. unicolor and Muraena helena) and banding homologies of nine chromosome pairs were pointed out. A single nucleolar organizer region (NOR) was localized by FISH, silver- and CMA3-staining on the short arm of the pair 12. Telomeric (TTAGGG) repeats were terminally localized by FISH on all the chromosome pairs. The genome size and the AT-DNA content were evaluated by flow cytometry. Available cytogenetic data on the Muraenidae were then compared and discussed

    Conventional karyotype, nucleolar organizer regions and genome size in five Mediterranean species of Syngnathidae (Pisces, Syngnathiformes)

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    Conventional karyotypes. NOR-bearing chromosomes by means of silver impregnation and genome size were investigated in five Mediterranean species in three genera of the Syngnathidae. A karyotype of 48 subtelocentric-acrocentric chromosomes was found in the seahorse Hippocampus hippocampus (FN=48) while a diploid value of 44 occurred in IL guttulatus (2 sm-m+42 a; FN=46) and the pipefish Syngnathus abaster (44 a: FN=44) and S. typhle (44 a: FN=44). The pipefish Nerophis ophidion, possessing a diploid chromosomal set of 58 made up of 50 meta-submetacentric and eight subteloacrocentric elements (FN=108) and a genome size three to four times larger than those known to date, differs cytogenetically from all other Syngnathids studied so far. A single pair of active NOR-bearing chromosomes was found in both species of the genus Hippocampus while in Syngnathus and Nerophis species more than two silver positive chromosomes were found to be involved in nucleolus organization giving rise to NOR-bearing chromosome polymorphism. The possible evolutionary routes of quantitative and qualitative changes in chromosome and DNA are discussed. The resulting phylogenetic scheme is shown to coincide with that constructed from morphological characters
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