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    Calcium carbonate precipitation by bacterial strains isolated from a limestone cave and from a loamy soil

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    To study the role of calcifying bacteria in monument protection, 31 calcifying bacterial strains were isolated from natural habitats: 64% were of the genus Bacillus, 16% of the genus Arthrobacter, and 2 of the remaining isolates were identified as Kingella and Xanthomonas. The ability to form CaCO3 crystals, the extent of the precipitation, and the type of crystals formed were determined at incubation temperatures of 4, 22, and 32±C. The highest of these temperatures favored CaCO3 formation. Most of the bacteria precipitated CaCO3 in the form of calcite. This activity was strictly controlled by the growth of microbial colonies on a solid substrate. The role of the calcifying bacteria in natural precipitation of carbonates is discussed. Further experiments are in progress in order to select the most suitable bacterial strains for a controlled production of calcareous crusts. Keywords adobe, biomineralization, calcifying bacteria, calcite precipitation, historic monument protection, historic monument restoratio

    Involvement of microorganisms in the formation of carbonate speleothems in the Cervo Cave (L'Aquila-Italy)

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    Much is known about the bacterial precipitation of carbonate rocks, but comparatively little is known about the involvement of microbes in the formation of secondary mineral structures in caves. We hypothesized that bacteria isolated from calcareous stalactites, which are able to mediate CaCO3 precipitation in vitro, play a role in the formation of carbonate speleothems.We collected numerous cultivable calcifying bacteria from calcareous speleothems from Cervo cave, implying that their presence was not occasional. The relative abundance of calcifying bacteria among total cultivable microflora was found to be related to the calcifying activity in the stalactites. We also determined the δ13C and δ18O values of the Cervo cave speleothems from which bacteria were isolated and of the carbonates obtained in vitro to determine whether bacteria were indeed involved in the formation of secondary mineral structures. We identified three groups of biological carbonates produced in vitro at 11◦C on the basis of their carbon isotopic composition: carbonates with δ13C values (a) slightly more positive, (b) more negative, and (c) much more negative than those of the stalactite carbonates. The carbonates belonging to the first group, characterized by the most similar δ13C values to stalactites, were produced by the most abundant strains. Most of calcifying isolates belonged to the genus Kocuria. Scanning electron microscopy showed that dominant morphologies of the bioliths were sherulithic with fibrous radiated interiors. We suggest a mechanism of carbonate crystal formation by bacteria
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