46 research outputs found
Development of an interspecies interaction model: An experiment on Clostridium cadaveris and Clostridium sporogenes under anaerobic condition
No full textThe specific primer and probe sets for quantifying Clostridiwn cadaveris and Clostridium sporogenes using a quantitative real-time PCR were designed. Each primer and probe set detected only the target species very specifically. The two species were cultivated in pure and mixed culture in batch mode with glucose as the only carbon source. The designed QPCR sets were used successfully to estimate the biokinetic parameters of each species in pure culture: i.e., maximum specific growth rate mu(max), half saturation concentration K-s, growth yield Y, and decay coefficient K-d. of C. cadaveris and C. sporogenes were 0.311 +/- 0.020 and 0.360 +/- 0.019 h(-1), 4.241 +/- 1.653 and 5.171 +/- 1.097 g/L, 0.301 +/- 0.065 and 0.199 +/- 0.037 10(11) copies/g, 0.005 0.043 and 0.009 +/- 0.025 h(-1), respectively. The effect of interspecific interaction of on substrate consumption rate and microbial growth was evaluated using mixed culture; curve fitting and comparison of coefficients detected increase in substrate consumption rate but decrease in microbial growth rate; these results imply interspecific interaction effect. A new model was of the interspecific interaction was developed, with focus on accuracy, realism, simplicity and biological significance. This interspecific interaction model may be extended to more complex bioprocesses such as biological wastewater treatment systems and anaerobic digestion.11Nsciescopu
Factors shaping microbial communities in field-scale thermophilic and mesophilic anaerobic digesters treating food wastewater
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Microbial communities underpinning anaerobic mono- and co-digestion of sewage sludge and food waste: a full-scale study
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Single and combined inhibition of Methanosaeta concilii by ammonia, sodium ion and hydrogen sulfide
No full textSingle and combined inhibition of lag time lambda and specific methanogenic activity R-CH4 of Methanosaeta concilii by NH3, Na+ and H2S were investigated using inhibition tests with a single inhibitor and a 3(3) full-factorial experiment of NH3, Na+ and H2S concentrations (1.5 <= total ammonia nitrogen (TAN)/L <= 4.5 g, 1 <= Na+/L <= 4.3 g, 14.2 <= total hydrogen sulfide sulfur (THSS)/L <= 836 mg). All three inhibitors significantly increased. and reduced R-CH4 of M. concilii. The half-maximal inhibitory concentrations of NH3, Na+ and H2S for M. concilii were 6.4 g TAN/L, 5.2 g Na+/L and 1.6 g THSS/L. Partial cubic models adequately approximated the corresponding response surfaces of lambda and R-CH4 from the 3(3) full-factorial experiment. The inhibitors inhibited R-CH4 synergistically, but inhibited lambda in a complex manner. The combination of NH3 and Na+ showed the strongest synergistic inhibition of both lambda and R-CH4.11Nsciescopu
Comprehensive analysis of microbial communities in full-scale mesophilic and thermophilic anaerobic digesters treating food waste-recycling wastewater
No full textMicrobes were sampled for a year in a full-scale mesophilic anaerobic digester ( MD) and a thermophilic anaerobic digester (TD) treating food waste-recycling wastewater (FRW), then microbial community structure, dynamics and diversity were quantified. In the MD, Fastidiosipila, Petrimonas, vadinBC27, Syntrophomonas, and Proteiniphilum were dominant bacterial genera; they may contribute to hydrolysis and fermentation. In the TD, Defluviitoga, Gelria and Tepidimicrobium were dominant bacteria; they may be responsible for hydrolysis and acid production. In the MD, dominant methanogens changed from Methanobacterium (17.1 +/- 16.9%) to Methanoculleus (67.7 +/- 17.8%) due to the increase in ammonium concentration. In the TD, dominant methanogens changed from Methanoculleus (42.8 +/- 13.6%) to Methanothermobacter (49.6 +/- 11.0%) due to the increase of pH. Bacteria and archaea were more diverse in the MD than in the TD. These results will guide development of microbial management methods to improve the process stability of MD and D treating FRW.11Nsciescopu
Magnetite as an enhancer in methanogenic degradation of volatile fatty acids under ammonia-stressed conditions
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Microbial communities underlying anaerobic mono- and co-digestion of sewage sludge and food waste in different mixture ratio
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Long term-monitoring of bacterial community structure and variation in biological nitrogen removal process
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Microbial characteristic in full scale anaerobic mono-and co-digestion treating animal waste and food waste: correlations between process parameters and microbial community
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